LOCUS       sequence-104487-        8924 bp    DNA     circular SYN 14-APR-2025
DEFINITION  AdenoAssociated Virus plasmid containing expanded repeats--CCTG 
            1200x that correspond to Myotonic Dystrophy of type 2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8924)
  TITLE     Charlet-Berguerand plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8924)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAAV CCTG 1200x
FEATURES             Location/Qualifiers
     source          1..8924
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     enhancer        221..524
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        525..728
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     679..699
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     773..792
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        773..791
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(5597..5621)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    5671..6147
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(5870..5889)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   6187..6327
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   6187..6316
                     /label=AAV2 ITR
     rep_origin      6402..6857
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6489..6508)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6699..6720
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6874..6893)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     6993..7015
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(7053..7071)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        7139..7243
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7244..7312,7313..8104)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7462..7481)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      8275..8863
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8764..8783
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgca cgcgtggagc tagttattaa tagtaatcaa
      181 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      241 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      301 ttcccatagt aacgtcaata gggactttcc attgacgtca atgggtggag tatttacggt
      361 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      421 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      481 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      541 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      601 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      661 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      721 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
      781 ctcactatag ggagacccaa gctggctagc gtttaaactt aagcttggta ccgagctcgg
      841 atccactagt ccagtgtggt ggaattctgc agatatccag cacagtggcg gccgctcgag
      901 tctagtggcc acgcgggcca tttccacgcg ggccatttcc tgcctgcctg cctgcctgcc
      961 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1021 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1081 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1141 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1201 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1261 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1321 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1381 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1441 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1501 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1561 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1621 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1681 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1741 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1801 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1861 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1921 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     1981 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2041 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2101 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2161 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2221 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2281 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2341 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2401 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2461 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2521 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2581 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2641 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2701 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2761 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2821 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2881 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     2941 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3001 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3061 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3121 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3181 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3241 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3301 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3361 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3421 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3481 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3541 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3601 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3661 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3721 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3781 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3841 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3901 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     3961 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4021 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4081 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4141 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4201 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4261 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4321 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4381 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4441 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4501 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4561 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4621 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4681 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4741 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4801 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4861 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4921 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     4981 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     5041 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     5101 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     5161 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     5221 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     5281 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     5341 tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc
     5401 tgcctgcctg cctgcctgcc tgcctgtgcc tgcctgcctg cctgcctgcc tgcctgcctg
     5461 cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg cctgcctgcc tgcctgcctg
     5521 cctgcctgcc tgcctgcctc ctaaatggaa atggccatta gggcccgttt aaacccgctg
     5581 atcagccggc gcgccgaccc agctttcttg tacaaagtgg ttgatgacct gcagaagctt
     5641 gcctcgagca gcgctgctcg agagatctac gggtggcatc cctgtgaccc ctccccagtg
     5701 cctctcctgg ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta
     5761 agttgcatca ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg
     5821 gtatggagca aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa
     5881 ccaagctgga gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa
     5941 gcgattctcc tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc
     6001 agctaatttt tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc
     6061 aactcctaat ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg
     6121 tgaaccactg ctcccttccc tgtccttctg attttgtagg taaccacgtg cggaccgagc
     6181 ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct cgctcgctca
     6241 ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga
     6301 gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc cttacgcatc
     6361 tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc
     6421 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct
     6481 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg
     6541 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga
     6601 ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt
     6661 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg
     6721 aacaacactc aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc
     6781 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat
     6841 attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     6901 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     6961 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     7021 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     7081 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     7141 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7201 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7261 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7321 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7381 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7441 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7501 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     7561 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     7621 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     7681 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     7741 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     7801 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     7861 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     7921 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     7981 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8041 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8101 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8161 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8221 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8281 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8341 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8401 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     8461 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     8521 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     8581 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     8641 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     8701 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     8761 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     8821 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     8881 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
