LOCUS       sequence-104617-         473 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expressed a nuclease dead Cas9 tagged with 10 copies of the GCN4 
            peptide v4 and BFP. This plasmid is part of the SunTag system for 
            gene activation .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 473)
  AUTHORS   Tanenbaum ME, Gilbert LA, Qi LS, Weissman JS, Vale RD
  TITLE     A Protein-Tagging System for Signal Amplification in Gene Expression
            and Fluorescence Imaging.
  JOURNAL   Cell. 2014 Oct 8. pii: S0092-8674(14)01227-6. doi: 
            10.1016/j.cell.2014.09.039.
  PUBMED    25307933
REFERENCE   2  (bases 1 to 473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Oct 8. pii: S0092-8674(14)01227-6. doi: 10.1016/j.cell.2014.09.039."
COMMENT     Sequence Label: pHRdSV40-dCas9-10xGCN4_v4-P2A-BFP
FEATURES             Location/Qualifiers
     source          1..473
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      286..295
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(344..364)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(440..459)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
ORIGIN
        1 agctgtcgtc caccttggcc atctcgttgc tgaagatctc ttgcagatag cagatccggt
       61 tcttccgtct ggtgtatctt cttctggcgg ttctcttcag ccgggtggcc tcggctgttt
      121 ctccgctgtc gaacagcagg gcgccgatca ggttcttctt gatgctgtgc cggtcggtgt
      181 tgcccagcac cttgaatttc ttgctgggca ccttgtactc gtcggtgatc acggcccagc
      241 ccacagagtt ggtgccgatg gccaggccga tgctgtactt cttgtccatg gtggcgatcc
      301 cgggcccgcg gtaccgcaag cttgatatcc tgcagacgcg tcccaccttg cgcttcttct
      361 tgggcatggt ggcgcgcgta gcctaggcct ccaaaaaagc ctcctcacta cttctggaat
      421 agctcagagg cagaggcggc ctcggcctct gcataaataa aaaaaattag tca
//
