LOCUS       sequence-104661-         786 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  MXS_chaining vector with mPlum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Sladitschek HL, Neveu PA
  TITLE     MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and 
            Flow Cytometry Approaches in Mammalian Systems.
  JOURNAL   PLoS One. 2015 Apr 24;10(4):e0124958. doi: 
            10.1371/journal.pone.0124958. eCollection 2015.
  PUBMED    25909630
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-04-24"; volume: "10(4):e0124958. doi"; pages: " 
            10.1371/journal.pone.0124958. eCollection 2015"
COMMENT     Sequence Label: MXS_mPlum
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(27..704)
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     complement(86..109)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(284..303)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gcgcacattt ccccgaattg gtcgacggcg ccggtggagt ggcggccctc ggcgcgctcg
       61 tactgttcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt gatgtcggtc
      121 ttgtaggcgc cgggcagctg cacgggcttc ttggccatgt aggtggtctt gacctcggcg
      181 tcgtagtggc cgccgtcctt cagcctcagc ctcatcttca tctcgccctt cagggcgccg
      241 tcctcggggt acatccgctc ggaggaggcc tcccagccca tggtcttctt ctgcattacg
      301 gggccgtcgg aggggaagtt ggtgccgcgc accttcacct tgtagatgaa ctcgccgtcc
      361 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
      421 tcccacttga agccctcggg gaaggacagc ttcaagtagt cggggatgtc ggcggggtgc
      481 ttcacgtagg ccttggagcc gtacatgatc tgaggggaca ggatgtccca ggcgaagggc
      541 agggggccac ccttggtcac cttcagcctg gcggtctggg tgccctcgta ggggcggccc
      601 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgctccttg
      661 aagcgcatga actccttgat gacctcctcg cccttgctca ccatctcgag ttacgcgttt
      721 gatatcatgg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc
      781 tcactc
//
