LOCUS       sequence-104758-        1115 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  bacterial expression of N-terminal fusion protein of CNA35 with 
            m-Turquoise-2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1115)
  AUTHORS   Aper SJ, van Spreeuwel AC, van Turnhout MC, van der Linden AJ, 
            Pieters PA, van der Zon NL, de la Rambelje SL, Bouten CV, Merkx M
  TITLE     Colorful Protein-Based Fluorescent Probes for Collagen Imaging.
  JOURNAL   PLoS One. 2014 Dec 9;9(12):e114983. doi: 
            10.1371/journal.pone.0114983. eCollection 2014.
  PUBMED    25490719
REFERENCE   2  (bases 1 to 1115)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2014-12-9"; volume: "9(12):e114983. doi"; pages: " 
            10.1371/journal.pone.0114983. eCollection 2014"
COMMENT     Sequence Label: pET28a-mTurquoise2-CNA35
FEATURES             Location/Qualifiers
     source          1..1115
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..51)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(1082..1103)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ttcctttcgg gctttgttag cagccggatc tcagtggtgg tggtggtggt gctcgagcaa
       61 tgccttacag gtcgactgaa gccttggtat ctttatcctg ttttaaaact tttaattcac
      121 cttttacagt accttcaata ccggcattag cattaatatt gtgcacagta tgattaaatg
      181 atttcccgtt cacttcttcc ttaccatgct cttgatacca agcttgtgaa ttattaacaa
      241 actctttttg ctgttcattc gtaattttgg ttttgtagtt aattgaaaaa ctattatatg
      301 acccatagcc ttgtggaatt gttacatcaa ttgtgttctt cgtattatca acagttattt
      361 tagaacctgg aaaggctttt tcaaaatcag taattgcact ttgtccacta taataattgc
      421 tatgtgtacc tgtcacatta atgtttaatg tgcttaaatc taactgctgt ccaccttgaa
      481 tctgatcctt tatagtaata tctttcgata cataactttt ttcattgtta atatttaaaa
      541 accatcgtac atgtgtcgta tcttctggta gcatatctcc cgttttataa tagaaaacac
      601 tacttgttcc cgcttcactt ttatgaaccg taacattcgt tgatttattc ccagatgtta
      661 tcgtagctac tttgtcatct gaagtatttg tttgcgttaa atttcttcct tgtacttcaa
      721 attctgcaaa tcccgaaaca tcacttaatt tttctacttt atcattgaat gtaattgttg
      781 caccgtctgg tgtaataact gcttgaccca cctgttcacc tttaacagtt aatggtactg
      841 ttttactata accctctatc tttactgtac cgcttgtcgg ccatgccact ttaatcatgt
      901 caccattttg tatttttcca tttttatcgt cgaacgtcat ttttactgtc gttttaccac
      961 catcttctat cttagacggt gatacagtta aatctgtaac attcgttgat gaaatatctc
     1021 gtgcggatcc gtggaattcc ttgtacagct cgtccatgcc gagagtgatc ccggcggcgg
     1081 tcacgaactc cagcaggacc atgtgatcgc gcttc
//
