LOCUS       sequence-104806-        2748 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  R57C10 (N-synaptobrevin), ""Pan-Neuronally,"" driven wild type yeast
            Flp2 promoter-fusion.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Nern A, Pfeiffer BD, Rubin GM
  TITLE     Optimized tools for multicolor stochastic labeling reveal diverse 
            stereotyped cell arrangements in the fly visual system.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 May 11. pii: 201506763.
  PUBMED    25964354
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2015 May 11. pii: 201506763."
COMMENT     Sequence Label: R57C10-Flp2
FEATURES             Location/Qualifiers
     source          1..2748
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(838..862)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             897..2168
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MPQFGILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRI"
     primer_bind     complement(2432..2448)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2432..2448)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 atttcccacc ccttggccat cggcacttgg aaatcgctaa ggaaaagctt ctcgctggaa
       61 caccattcgc tgctggccat ccaggcagct ttttccccaa atacacataa cagttaggaa
      121 agctgcaagt tttcgcagtt ggccaaaaac gtcgtaagtg aaaattttcc tggtgctgcg
      181 taaaaaaatt cgtaaaatat tagttaagcc tggtttttgt aaactcaagt tgcccgagca
      241 aaagtaaagt gattctgatc aaaagtgcta aaactgattg attaggaaaa cgaagatcaa
      301 acgaaatgtc aaccaaagtc agcgaaaacc cttgtcgtgc agaatttcat tgattttttc
      361 ttctgagccc acgttggcca gttgggcact tgaaattatt tttaaaacgt tttgcatttt
      421 gaagacatct taggtgcccc tgggggtatg caacggaaat gaggaaggac atgcctcatg
      481 gctgggcgtg ttgctgccac ccaaatagtc ctcttacgtc ccaattagaa tcccatagct
      541 gcccatttca agcattaacc gatgtactaa aaatagttgc aactattttc gcactctatt
      601 cagcagcagt tccagcgctg agccagaaaa ttcctgcgaa aggctgtcgt ggcaagtgtt
      661 acccgcacac atacacatca tccagatcca gccaatcccc ccgtggagtg gagtgaattt
      721 tcattgacga cggggcgaaa aagctctggc ggaagagcga cagagagaga gagaaacaga
      781 gagagagaga gagaagcggc actgagagcg caaccctcta gaacaagggc gaattcgacc
      841 cagctttctt gtacaaagtg gtgataaacg gccggccaga tctggtacca atcaaaatgc
      901 cacaatttgg tatattatgt aaaacaccac ctaaggtgct tgttcgtcag tttgtggaaa
      961 ggtttgaaag accttcaggt gagaaaatag cattatgtgc tgctgaacta acctatttat
     1021 gttggatgat tacacataac ggaacagcaa tcaagagagc cacattcatg agctataata
     1081 ctatcataag caattcgctg agtttcgata ttgtcaataa atcactccag tttaaataca
     1141 agacgcaaaa agcaacaatt ctggaagcct cattaaagaa attgattcct gcttgggaat
     1201 ttacaattat tccttactat ggacaaaaac atcaatctga tatcactgat attgtaagta
     1261 gtttgcaatt acagttcgaa tcatcggaag aagcagataa gggaaatagc cacagtaaaa
     1321 aaatgcttaa agcacttcta agtgagggtg aaagcatctg ggagatcact gagaaaatac
     1381 taaattcgtt tgagtatact tcgagattta caaaaacaaa aactttatac caattcctct
     1441 tcctagctac tttcatcaat tgtggaagat tcagcgatat taagaacgtt gatccgaaat
     1501 catttaaatt agtccaaaat aagtatctgg gagtaataat ccagtgttta gtgacagaga
     1561 caaagacaag cgttagtagg cacatatact tctttagcgc aaggggtagg atcgatccac
     1621 ttgtatattt ggatgaattt ttgaggaatt ctgaaccagt cctaaaacga gtaaatagga
     1681 ccggcaattc ttcaagcaat aaacaggaat accaattatt aaaagataac ttagtcagat
     1741 cgtacaataa agctttgaag aaaaatgcgc cttattcaat ctttgctata aaaaatggcc
     1801 caaaatctca cattggaaga catttgatga cctcatttct ttcaatgaag ggcctaacgg
     1861 agttgactaa tgttgtggga aattggagcg ataagcgtgc ttctgccgtg gccaggacaa
     1921 cgtatactca tcagataaca gcaatacctg atcactactt cgcactagtt tctcggtact
     1981 atgcatatga tccaatatca aaggaaatga tagcattgaa ggatgagact aatccaattg
     2041 aggagtggca gcatatagaa cagctaaagg gtagtgctga aggaagcata cgataccccg
     2101 catggaatgg gataatatca caggaggtac tagactacct ttcatcctac ataaatagac
     2161 gcatataacc taggaagctt atcgataccg tcgactaaag ccaaatagaa aattattcag
     2221 ttcctggctt aagtttttaa aagtgatatt atttatttgg ttgtaaccaa ccaaaagaat
     2281 gtaaataact aatacataat tatgttagtt ttaagttagc aacaaattga ttttagctat
     2341 attagctact tggttaataa atagaatata tttatttaaa gataattgcg tttttattgt
     2401 cagggagtga gtttgcttaa aaactcgttt agatccacta gttctagagc ggccgctggc
     2461 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc
     2521 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca
     2581 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc
     2641 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gaggcgcgcc ctagttccag
     2701 tgaaatccaa gcattttcta aattaatgta ttcttattat tatagttg
//
