LOCUS       sequence-105816-         702 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Fluorescent reporter for F-actin LifeAct-mCherry expressed under 
            MtBCP1 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Ivanov S, Harrison MJ
  TITLE     A set of fluorescent protein-based markers expressed from 
            constitutive and arbuscular mycorrhiza-inducible promoters to label 
            organelles, membranes and cytoskeletal elements in Medicago 
            truncatula.
  JOURNAL   Plant J. 2014 Oct 20. doi: 10.1111/tpj.12706.
  PUBMED    25329881
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2014 Oct 20. doi: 10.1111/tpj.12706."
COMMENT     Sequence Label: pCMB-ACTLr
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(175..199)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(430..450)
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     primer_bind     complement(458..475)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(458..474)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(467..489)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     misc_feature    563..587
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
       61 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
      121 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aaggacccag
      181 ctttcttgta caaagtggaa taagcggcca tgctagagtc cgcaaaaatc accagtctct
      241 ctctacaaat ctatctctct ctatttttct ccagaataat gtgtgagtag ttcccagata
      301 agggaattag ggttcttata gggtttcgct catgtgttga gcatataaga aacccttagt
      361 atgtatttgt atttgtaaaa tacttctatc aataaaattt ctaattccta aaaccaaaat
      421 ccagtgacgc aactttatta tacatagttg ataattcact ggccgtcgtt ttacaacgtc
      481 gtgactggga aaacgagctc aagcttagct tgagcttgga tcagattgtc gtttcccgcc
      541 ttcagtttaa actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga
      601 gcgtttatta gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat
      661 ttgtatgtgc atgccaacca cagggttccc ctcgggatca aa
//
