LOCUS       sequence-105993-       13119 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13119)
  AUTHORS   Sarbassov DD, Ali SM, Kim DH, Guertin DA, Latek RR, 
            Erdjument-Bromage H, Tempst P, Sabatini DM
  TITLE     Rictor, a novel binding partner of mTOR, defines a 
            rapamycin-insensitive and raptor-independent pathway that regulates 
            the cytoskeleton.
  JOURNAL   Curr Biol 2004 Jul 27;14(14):1296-302.
  PUBMED    15268862
REFERENCE   2  (bases 1 to 13119)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Curr Biol";
            date: "2004-07-27"; volume: "14(14)"; pages: "1296-302"
COMMENT     Sequence Label: myc-mTOR
FEATURES             Location/Qualifiers
     source          1..13119
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..98)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      892..1480
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1381..1400
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1634..1651
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1768..1789
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1804..1809,1810..1827,1828..1834)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1842..1858
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1847..1869
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1866..1882
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1866..1882
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     enhancer        1911..2290
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2291..2494
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2445..2465
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2491..2515
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        2725..2743
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     2725..2742
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     2756..2775
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      2821..2830
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2830..2859
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     regulatory      5714..5723
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8935..9207
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
     regulatory      11316..11325
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    11329..11463
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11366..11385)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     11420..11439
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        11532..11889
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        11532..11723
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(11555..11575)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      11740..11875
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(11909..11926)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(11909..11925)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11918..11940)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      12138..12593
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(12225..12244)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     12435..12456
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(12610..12629)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     12729..12751
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(12789..12807)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        12875..12979
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12980..13048,13049..13119,1..721)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
       61 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
      121 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      181 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      241 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
      301 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
      361 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
      421 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
      481 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
      541 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
      601 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
      661 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
      721 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
      781 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
      841 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
      901 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
      961 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1021 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1081 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1141 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1201 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1261 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1321 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1381 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1441 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1501 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1561 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1621 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     1681 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     1741 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     1801 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     1861 tcacacagga aacagctatg acatgattac gaattaattc gagctcgccc gacattgatt
     1921 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     1981 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     2041 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     2101 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     2161 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     2221 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     2281 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     2341 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     2401 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     2461 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
     2521 gagacgccat ccacgctgtt ttgacctcca tagaagacac cgggaccgat ccagcctccg
     2581 cggccgggaa cggtgcattg gaacgcggat tccccgtgcc aagagtgacg taagtaccgc
     2641 ctatagagtc tataggccca cccccttggc ttcgttagaa cgcggctaca attaatacat
     2701 aaccttatgt atcatacaca tacgatttag gtgacactat agaataacat ccactttgcc
     2761 tttctctcca caggtgtcca ctcccaggtc caactgcacc tcggttctat cgataagctt
     2821 gccaccatgg aacaaaagct cattagcgag gaagacctta acatgcttgg gacaggccct
     2881 gccacggcca ccgccggtgc cgccacatct agcaacgtga gcgttctgca gcagttcgcc
     2941 agtgggctga agagccggaa tgaggagacc agggccaaag cagccaagga gctccagcac
     3001 tatgtcacca tggaacttcg agagatgagt caggaggagt ctactcgctt ctatgaccag
     3061 ctgaaccatc acatttttga actggtttcc agctcagacg ccaatgagag gaagggtggc
     3121 atcttggcca ttgccagcct cattggagtg gaaggtggga attccaccag gattggcaga
     3181 tttgccaact accttcggaa cctcctcccc tcaagtgatc cagttgtcat ggagatggca
     3241 tccaaggcca ttggccgcct tgcaatggca ggggacactt tcactgctga gtatgtggag
     3301 tttgaagtga agcgagcctt ggagtggctg ggtgctgacc gaaatgaggg ccggagacat
     3361 gcagctgtcc tcgttctccg tgagctggcc atcagcgtcc ccaccttctt cttccagcaa
     3421 gttcagccct tctttgacaa catttttgtg gctgtgtggg accccaagca ggccatccgt
     3481 gaaggagctg tggctgccct tcgtgcctgt ctgattctta ccacgcagcg ggagccgaag
     3541 gagatgcaga agcctcagtg gtacaggcac acatttgaag aagcagagaa aggttttgat
     3601 gagaccctgg ccaaagagaa gggtatgaac cgagatgatc gaatccacgg ggccttgctg
     3661 atcctcaacg agctcgtccg aatcagcagc atggagggag agcgtctgag agaggagatg
     3721 gaggaaatca cccagcagca gctggtacat gacaagtact gcaaagacct aatgggcttt
     3781 gggacaaagc ctcggcacat cactcccttc accagcttcc aggctgtgca gccccagcag
     3841 tcaaacgcct tggtgggact gctggggtac agctcccacc aaggcctaat ggggtttggg
     3901 gcttccccca gccctacaaa gtccactctg gtggaaagcc gttgttgcag agacttgatg
     3961 gaagagaaat ttgatcaggt gtgccagtgg gtgctgaaat gtaggagcag caagaactca
     4021 ctgatccaaa tgacaatcct taatctgttg ccccgcttgg ctgcattccg accgtctgcc
     4081 ttcacagata cccagtacct gcaagacacc atgaaccatg tcctgagctg tgtcaagaag
     4141 gaaaaggaac ggaccgcagc gttccaggcc ctagggctgc tttctgtggc ggtgaggtcc
     4201 gagtttaagg tctacctgcc ccgagtactt gacatcatcc gagcagccct gcctccaaag
     4261 gactttgccc acaagaggca gaaaactgtg caggtggatg ccacagtgtt cacgtgcatc
     4321 agcatgctgg cgcgggccat ggggccaggc atccagcagg acatcaagga gctgctggag
     4381 cccatgttgg cagtgggcct gagccctgcg ctcactgctg tgctctatga cctgagccgg
     4441 cagattccgc agctgaagaa agatattcag gacgggcttc tgaagatgct gtccctggtc
     4501 cttatgcaca aacccctgcg gcacccgggc atgcccaaag gcctggccca ccagctggcc
     4561 tccccaggtc ttaccaccct ccctgaggcc agcgacgtgg ccagcatcac tcttgccctt
     4621 cgaactctcg gcagctttga atttgaaggc cactctctga cccagttcgt ccgacactgc
     4681 gcagatcatt tcctgaacag tgagcacaag gagatccgca tggaagcagc ccgcacctgc
     4741 tcccgcctgc tcacaccctc catccacctc atcagcggcc atgcccatgt ggttagccag
     4801 accgcagtgc aagtggtagc agatgtgctc agcaagctgc ttgtggtcgg cataacagat
     4861 cctgatcctg atatccgcta ctgtgtcttg gcctccctgg atgagcgctt cgatgcccac
     4921 ttggcccagg cagaaaactt acaagctctg tttgtggctc tgaatgacca ggtctttgag
     4981 atccgagagc tggccatctg cactgtgggc cgactcagta gcatgaaccc agcctttgtc
     5041 atgcctttcc tgcgcaagat gctcatccag attttgacag agctggagca cagtggcatt
     5101 gggagaatca aggagcagag tgcccgcatg ctggggcacc tggtctccaa tgccccccgc
     5161 ctcatccgcc cctatatgga gcctattctg aaggctttaa ttttgaaact caaagatcca
     5221 gaccctgacc caaacccggg cgtgatcaat aacgtgttgg ccactatagg agaactggct
     5281 caggttagcg gcctggagat gaggaagtgg gtggacgagc tctttgtcat catcatggac
     5341 atgctgcagg actcctctct tctggccaaa agacaggtgg ctttgtggac cctgggacag
     5401 ttggtggcca gtactggcta cgtggtggag ccctacagga agtaccccac tctgcttgaa
     5461 gtgctgctga attttctgaa gacggagcag aaccagggca ctcggagaga ggccatccga
     5521 gtgttagggc tcctcggggc tttggacccc tacaagcaca aagtgaacat cggcatgatt
     5581 gaccagtccc gagatgcttc tgctgtcagc ctgtcagaat ccaagtcaag tcaagattcc
     5641 tctgactaca gcaccagtga aatgctggtc aacatgggaa acctgccact ggacgagttc
     5701 taccccgccg tgtccatggt ggccttgatg cggatcttcc gagaccagtc cctctctcac
     5761 caccacacca tggtggttca ggccatcacc ttcatcttca agtccctggg gctcaagtgt
     5821 gtgcagttcc tgccccaggt catgcccacg ttccttaacg tcatccgagt ctgtgatggg
     5881 gccatccggg aatttctgtt ccagcagctg ggaatgctgg tgtcctttgt gaagagccac
     5941 atccgtccct acatggatga aatagtcacc ctcatgagag aattttgggt catgaacacc
     6001 tcaatccaga gcacaatcat tcttctcatt gagcaaatcg tggtggctct tggaggtgaa
     6061 tttaagctct acctgcccca gctgatccca cacatgctgc gtgtcttcat gcatgacaac
     6121 agccagggcc gcatagtctc catcaagctg ttagcagcga tccagctgtt tggcgccaac
     6181 ctggatgact atctgcactt gttgttgcct ccgatcgtga aattgtttga tgcccctgaa
     6241 gttccactgc cgtcgagaaa ggcagcgttg gagacagtgg accgcctgac agagtccctg
     6301 gatttcactg actatgcctc ccgcatcatt cacccgattg ttcgcacgct agaccagagc
     6361 ccagagctgc gctccacagc catggacacc ctgtcttcac ttgtgtttca actagggaag
     6421 aagtaccaga tcttcattcc aatggtgaat aaagtccttg tgcgacaccg gatcaatcac
     6481 cagcgctacg acgtgctgat ctgcagaatc gtcaaggggt acacgcttgc tgatgaagaa
     6541 gaagaccctt tgatttacca gcatcgaatg ctaaggagca gccagggaga tgccctggcc
     6601 agtggaccag ttgaaacagg acccatgaag aaactgcatg tcagcaccat caacctccaa
     6661 aaggcctggg gagctgccag aagggtctcc aaggacgact ggctggagtg gctgcgacgc
     6721 ttgagtctgg agctgctgaa ggattcctca tcaccttccc tgcgctcatg ctgggccctg
     6781 gcccaggcct acaaccccat ggccagggat ctcttcaacg ctgcgtttgt gtcctgctgg
     6841 tctgaactga atgaagacca acaagatgag ctcatcagga gcattgagtt ggctctcact
     6901 tctcaagaca ttgctgaagt cacacaaacc ctcttgaact tggctgagtt catggagcac
     6961 agtgacaagg gccccctacc actgagagat gacaatggca tcgtgctgtt gggtgagaga
     7021 gctgccaagt gccgggcata tgccaaagca ctacactaca aagagctgga gttccagaag
     7081 gggcccacgc ctgccatact tgagtccctc atcagcatta ataataaact gcagcagcct
     7141 gaggcagcgt ccggggtgtt agagtacgcc atgaaacact tcggagagct ggagatccag
     7201 gccacctggt atgagaagtt gcatgagtgg gaggacgccc ttgtggccta cgacaagaag
     7261 atggacacga acaaggatga cccagagctg atgctgggcc gcatgcgctg tctcgaggcc
     7321 ttgggagaat ggggccagct tcatcagcag tgctgtgaaa agtggactct ggttaatgac
     7381 gagacccagg ctaagatggc ccggatggct gctgcagcag catggggttt aggtcagtgg
     7441 gacagcatgg aggagtacac ctgtatgatt cctcgggata ctcacgatgg agcattctac
     7501 agagcagtgt tggcactgca tcaggatctc ttctccttgg ctcaacagtg cattgacaag
     7561 gccagggacc tgctggacgc cgagctgact gccatggcag gggagagcta cagccgagcc
     7621 tatggggcca tggtttcttg ccacatgctg tccgagctgg aggaggttat ccagtacaaa
     7681 ctcgtcccgg agcgacggga gatcatccgc cagatctggt gggagagact gcagggctgc
     7741 cagcgtattg tagaggactg gcagaaaatc ctcatggtcc ggtcccttgt ggtcagccct
     7801 cacgaggaca tgagaacctg gctcaagtac gcaagcctgt gtggcaagag cggcagactg
     7861 gctcttgctc ataaaacctt agtgttgctc ttgggagttg atccatctcg gcaacttgac
     7921 catcctctgc caacagttca ccctcaagtg acctatgcct acatgaaaaa catgtggaaa
     7981 agcgctcgga agattgatgc cttccagcac atgcagcact ttgtgcagac catgcagcag
     8041 caggcccagc acgccattgc cacagaggac cagcagcaca agcaggagct gcataagctc
     8101 atggccaggt gttttctgaa acttggggag tggcagctga acctccaggg catcaacgag
     8161 agcaccatcc ccaaggtgct acagtactac agtgctgcca cagagcatga ccgcagctgg
     8221 tataaggctt ggcacgcatg ggcagtgatg aactttgaag ccgtgctaca ctacaaacat
     8281 cagaaccaag cccgcgatga gaagaagaaa ctgcgccatg ccagcggggc caacatcacc
     8341 aatgccacca ccactgccac caccgctgcc tccgctgccg ctgccaccag cacagagggc
     8401 agcaacagtg aaagtgaagc cgagagcaat gagagcagcc ccaccccgtc ccctctgcag
     8461 aagaaggtca ctgaggattt gtccaaaacc ctcttgttgt acactgtccc tgctgtccaa
     8521 ggcttcttcc gttctatctc cttgtcgaga ggcaacaacc tccaggatac actcagagtc
     8581 ctcaccttgt ggtttgatta tggtcactgg ccagatgtca atgaagccct ggtggaaggg
     8641 gtgaaggcca tacagattga cacttggtta caggttatac ctcagctcat tgcaagaatt
     8701 gacacgccca gacccttggt gggccggctc attcaccagc tcctcacaga tattggtcgg
     8761 taccacccac aggccctcat ctaccccctg acggtggctt ctaagtctac caccacagcc
     8821 cgtcacaatg cagccaacaa gatcctgaag aacatgtgcg agcacagcaa cacgctagtc
     8881 cagcaggcca tgatggtgag tgaagagctg attcgagtag ccatcctctg gcatgagatg
     8941 tggcatgaag gcctagaaga ggcctctcgc ttgtactttg gggagaggaa cgtcaaaggc
     9001 atgtttgagg tgctggagcc cctgcatgct atgatggaac gcggtcccca gaccctgaag
     9061 gaaacgtcct ttaatcaggc atatggtcga gatttaatgg aggcacaaga atggtgccga
     9121 aagtacatga aatcagggaa cgtcaaggac ctcacccaag cctgggacct ctactatcac
     9181 gtgttcagac ggatctccaa gcagctacca cagctcacat ccctggagct gcagtatgtg
     9241 tcccccaaac ttttgatgtg cagagacctt gaattggctg tgccaggaac atatgacccc
     9301 aaccagccaa tcattcgcat tcagtccata gccccgtctt tgcaagtcat cacatccaag
     9361 cagaggcctc ggaagctgac cctgatgggc agcaatgggc acgagtttgt tttcctcctg
     9421 aaaggccatg aagatctgcg gcaggacgag cgagtgatgc agctctttgg cctggtgaac
     9481 acactcctag ccaatgaccc aacttctctt cgaaagaacc tcagcatcca gagatacgcc
     9541 gtcattcctc tgtccaccaa ctcgggcctg attggctggg tgccccactg tgacacgctg
     9601 catgccctca tccgggacta cagagagaag aagaagatcc tgctgaacat cgagcaccgc
     9661 atcatgctgc ggatggctcc tgactatgac cacctgactc tgatgcagaa ggtggaggtg
     9721 tttgagcatg ctgtcaacaa cacagccggg gatgacctgg ccaagctgct gtggctgaaa
     9781 agccccagct cagaggtgtg gtttgaccga agaaccaatt atactcgctc cctggctgtc
     9841 atgtccatgg ttggatacat tttaggcctt ggagacaggc acccatccaa cctgatgctg
     9901 gaccggctga gtggaaagat cctgcacatt gactttgggg actgctttga ggttgctatg
     9961 accagagaga aatttccaga aaagattcca tttagactaa caagaatgtt gaccaatgct
    10021 atggaggtta ccggtctcga tggcaactat agaaccacat gccacacagt gatggaggtg
    10081 cttcgggagc acaaggacag tgtcatggct gtgctagaag cctttgtcta tgaccctctg
    10141 ctgaattgga ggctgatgga cacaaatgcc aaaggcaaca agcggtcccg aaccaggaca
    10201 gactcctatt ctgcaggcca gtcagtagaa attttggacg gtgtagaact tggagaacca
    10261 gcccataaga aaacagggac cactgtgcca gaatccatcc attctttcat tggagatggt
    10321 ttggtgaaac cagaagcctt aaacaagaaa gctattcaga ttattaacag ggttcgagat
    10381 aagctcactg gtcgggattt ctctcatgat gacactttgg atgttccaac ccaagtggaa
    10441 ctgcttatca agcaagcgac atctcatgag aacctctgcc agtgctacat tggctggtgt
    10501 cctttctggt aaccaaggcc tggcaaagaa aatcatctcc tccgatgctt ttgtaccttg
    10561 gtctgtgctt ccagtggact gaaaccatgg tcataaagtt ggactttgtt aatattttga
    10621 aatgtatatg aaaagaacta ctgtatattc aaagttggct tatgccaacc tcctagctgc
    10681 tgttgaaaag acactgtcag aaacacaagg cttgattcag ttcccaggac agtgaaacac
    10741 agtaatccta cagaaaccaa gcctttgatt ttgggagaac agaagatgag taactgacta
    10801 agaaatacgg gttttgactt aacttacaga agaactcatc atacgcattt gctgaccgaa
    10861 taatctagtt gatcctctca accaggggct tcaacagcaa ggacacagat gtcagcactc
    10921 caccatcctg ttacctcacc cgtccctgga tgcagtggca acatctgcag gatgggccac
    10981 cgtgtgtgta agaagatctg tcttccacct gatcccatga tgctgaacct cacagagccg
    11041 gccttccagg aaggacgttt gctcagacgc ctggccaccg aggatgagca ggtgtgccag
    11101 gatctcagtg cagggtccac gctggccctg ctgctgtgtt cagtgaggga tggatatgtt
    11161 gtgtttgcag cagggactca gaacacaaat gcttttgtgg aagtgctgat ctcagaggga
    11221 cactagcgca ggttgtgaat taagagcaaa gtaaatatcc aactaaacac aaagtataag
    11281 tgaagccaca tctagagtcg acctgcagaa gcttggccgc catggcccaa cttgtttatt
    11341 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
    11401 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
    11461 atcgggaatt aattcggcgc agcaccatgg cctgaaataa cctctgaaag aggaacttgg
    11521 ttaggtacct tctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
    11581 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
    11641 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
    11701 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
    11761 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
    11821 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
    11881 ttttgcaaaa agctgttaac agcttggcac tggccgtcgt tttacaacgt cgtgactggg
    11941 aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc
    12001 gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg
    12061 aatggcgcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac
    12121 gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
    12181 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
    12241 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
    12301 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
    12361 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
    12421 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
    12481 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
    12541 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg
    12601 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
    12661 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
    12721 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
    12781 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
    12841 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
    12901 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
    12961 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
    13021 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
    13081 tgaagatcag ttgggtgcac gagtgggtta catcgaact
//
