LOCUS       sequence-106-exp        4486 bp    DNA     circular SYN 14-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 4486)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD95_77
FEATURES             Location/Qualifiers
     source          1..4486
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(163..191)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     939..959
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1926..1948)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2048..2067
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2145..2163)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2231..2335
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2336..2404,2405..3196)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2554..2573)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3367..3955
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3856..3875
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4109..4126
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4243..4264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4279..4284,4285..4302,4303..4309)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4317..4333
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4322..4344
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4341..4357
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4341..4357
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 aagcttgcat gcctgcaggt cgactctaga ggatccccgg gattggccaa aggacccaaa
       61 ggtatgtttc gaatgatact aacataacat agaacatttt caggaggacc cttgagggta
      121 ccggtagaaa aaatgagtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      181 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      241 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      301 tgggtaagtt taaacatata tatactaact aaccctgatt atttaaattt tcagccaaca
      361 cttgtcacta ctttctgtta tggtgttcaa tgcttctcga gatacccaga tcatatgaaa
      421 cggcatgact ttttcaagag tgccatgccc gaaggttatg tacaggaaag aactatattt
      481 ttcaaagatg acgggaacta caagacacgt aagtttaaac agttcggtac taactaacca
      541 tacatattta aattttcagg tgctgaagtc aagtttgaag gtgataccct tgttaataga
      601 atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa
      661 tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa
      721 gttgtaagtt taaacatgat tttactaact aactaatctg atttaaattt tcagaacttc
      781 aaaattagac acaacattga agatggaagc gttcaactag cagaccatta tcaacaaaat
      841 actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc cacacaatct
      901 gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga gtttgtaaca
      961 gctgctggga ttacacatgg catggatgaa ctatacaaat agcattcgta gaattccaac
     1021 tgagcgccgg tcgctaccat taccaacttg tctggtgtca aaaataatag gggccgctgt
     1081 catcagagta agtttaaact gagttctact aactaacgag taatatttaa attttcagca
     1141 tctcgcgccc gtgcctctga cttctaagtc caattactct tcaacatccc tacatgctct
     1201 ttctccctgt gctcccaccc cctatttttg ttattatcaa aaaacttctc ttaatttctt
     1261 tgttttttag cttcttttaa gtcacctcta acaatgaaat tgtgtagatt caaaaataga
     1321 attaattcgt aataaaaagt cgaaaaaaat tgtgctccct ccccccatta ataataattc
     1381 tatcccaaaa tctacacaat gttctgtgta cacttcttat gttttttact tctgataaat
     1441 ttttttgaaa catcatagaa aaaaccgcac acaaaatacc ttatcatatg ttacgtttca
     1501 gtttatgacc gcaattttta tttcttcgca cgtctgggcc tctcatgacg tcaaatcatg
     1561 ctcatcgtga aaaagttttg gagtattttt ggaatttttc aatcaagtga aagtttatga
     1621 aattaatttt cctgcttttg ctttttgggg tttcccctat tgtttgtcaa gatttcgagg
     1681 acggcgtttt tcttgctaaa atcacaagta ttgatgagca cgatgcaaga aagatcggaa
     1741 gaaggtttgg gtttgaggct cagtggaagg tgagtagaag ttgataattt gaaagtggag
     1801 tagtgtctat ggggtttttg ccttaaatga cagaatacat tcccaatata ccaaacataa
     1861 ctgtttccta ctagtcggcc gtacgggccc tttcgtctcg cgcgtttcgg tgatgacggt
     1921 gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc
     1981 gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt
     2041 aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg
     2101 cacagatgcg taaggagaaa ataccgcatc aggcggcctt aagggcctcg tgatacgcct
     2161 atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     2221 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     2281 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     2341 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     2401 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     2461 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     2521 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     2581 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     2641 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     2701 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     2761 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     2821 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     2881 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     2941 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     3001 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     3061 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     3121 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     3181 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     3241 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     3301 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     3361 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     3421 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     3481 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     3541 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     3601 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     3661 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     3721 aacgacctac accgaactga gatacctaca gcgtgagcat tgagaaagcg ccacgcttcc
     3781 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     3841 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     3901 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     3961 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     4021 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     4081 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     4141 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     4201 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
     4261 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
     4321 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agctgtaagt
     4381 ttaaacatga tcttactaac taactattct catttaaatt ttcagagctt aaaaatggct
     4441 gaaatcactc acaacgatgg atacgctaac aacttggaaa tgaaat
//
