LOCUS       sequence-106122-         729 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing a CMV 
            promoter and renilla luciferase module. Compatible with MultiSite 
            Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV Renilla Luciferase L5-L2
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(27..46)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(28..46)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(91..294)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(120..140)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        295..674
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     protein_bind    complement(702..722)
                     /gene="mutant version of attB"
                     /label=attB5
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
ORIGIN
        1 gtcatggtgg ctagaagctt gggtctccct atagtgagtc gtattaattt cgataagcca
       61 gtaagcagtg ggttctctag ttagccagag agctctgctt atatagacct cccaccgtac
      121 acgcctaccg cccatttgcg tcaatggggc ggagttgtta cgacattttg gaaagtcccg
      181 ttgattttgg tgccaaaaca aactcccatt gacgtcaatg gggtggagac ttggaaatcc
      241 ccgtgagtca aaccgctatc cacgcccatt gatgtactgc caaaaccgca tcaccatggt
      301 aatagcgatg actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta
      361 ctgggcataa tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg
      421 catatgatac acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg
      481 acgtcaatgg aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa
      541 tgggcggggg tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg
      601 gaactccata tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt
      661 caataatcaa tgtcaacgcg tatatctggc ccgtacatcg ccaacttttg tatacaaagt
      721 tggcattat
//
