LOCUS       sequence-106549-        2813 bp    DNA     circular SYN 15-APR-2025
DEFINITION  A member of an RBS library for mRFP1 with different translation 
            rates.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 66 to 100)
  AUTHORS   Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM
  TITLE     Efficient search, mapping, and optimization of multi-protein genetic
            systems in diverse bacteria.
  JOURNAL   Mol Syst Biol. 2014 Jun 21;10:731. doi: 10.15252/msb.20134955.
  PUBMED    24952589
REFERENCE   2  (bases 1 to 2813)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-06-21"; volume: "10:731. doi"; pages: " 
            10.15252/msb.20134955"
COMMENT     Sequence Label: mRFP1-1
FEATURES             Location/Qualifiers
     source          1..2813
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        66..100
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     CDS             143..820
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     terminator      865..936
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      952..979
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      1021..1107
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     rep_origin      complement(1272..1860)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1352..1371)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1949..2043)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(2064..2723)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2638..2657
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     promoter        complement(join(2724..2813,1..13))
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
ORIGIN
        1 tacgtgccga tcaacgtctc attttcgcca gatatcctcg agcgcggaat tccctagggg
       61 atccgttgac ggctagctca gtcctaggta cagtgctagc ttctagaaac gacgtcgacg
      121 atcacaactt gatgtcgtat tcatggcgag ctctgaagac gttatcaaag agttcatgcg
      181 tttcaaagtt cgtatggaag gttccgttaa cggtcacgag ttcgaaatcg aaggtgaagg
      241 tgaaggtcgt ccgtacgaag gtacccagac cgctaaactg aaagttacca aaggtggtcc
      301 gctgccgttc gcttgggaca tcctgtcccc gcagttccag tacggttcca aagcttacgt
      361 taaacacccg gctgacatcc cggactacct gaaactgtcc ttcccggaag gtttcaaatg
      421 ggaacgtgtt atgaacttcg aagacggtgg tgttgttacc gttacccagg actcctccct
      481 gcaagacggt gagttcatct acaaagttaa actgcgtggt accaacttcc cgtccgacgg
      541 tccggttatg cagaaaaaaa ccatgggttg ggaagcttcc accgaacgta tgtacccgga
      601 agacggtgct ctgaaaggtg aaatcaaaat gcgtctgaaa ctgaaagacg gtggtcacta
      661 cgacgctgaa gttaaaacca cctacatggc taaaaaaccg gttcagctgc cgggtgctta
      721 caaaaccgac atcaaactgg acatcacctc ccacaacgaa gactacacca tcgttgaaca
      781 gtacgaacgt gctgaaggtc gtcactccac cggtgcttaa taaggccggc cgtgctagtg
      841 tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
      901 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
      961 tcgggtgggc ctttctgcgt ttatacaatt gactagtctg cagaaaagcg gccgcggcat
     1021 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     1081 gtgaacgctc tcctgagtag gacaaatccg ccgccctaga gcatgcgcgt tcggctgcgg
     1141 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     1201 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     1261 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     1321 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     1381 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     1441 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     1501 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     1561 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     1621 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     1681 aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     1741 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     1801 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     1861 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     1921 gggattttgg tcatgcccgg gtgcttggat tctcaccaat aaaaaacgcc cggcggcaac
     1981 cgagcgttct gaacaaatcc agatggagtt ctgaggtcat tactggatct atcaacagga
     2041 gtccaagcgg taccgatatc aaattacgcc ccgccctgcc actcatcgca gtactgttgt
     2101 aattcattaa gcattctgcc gacatggaag ccatcacaaa cggcatgatg aacctgaatc
     2161 gccagcggca tcagcacctt gtcgccttgc gtataatatt tgcccatggt gaaaacgggg
     2221 gcgaagaagt tgtccatatt ggccacgttt aaatcaaaac tggtgaaact cacccaggga
     2281 ttggctgaga cgaaaaacat attctcaata aaccctttag ggaaataggc caggttttca
     2341 ccgtaacacg ccacatcttg cgaatatatg tgtagaaact gccggaaatc gtcgtggtat
     2401 tcactccaga gcgatgaaaa cgtttcagtt tgctcatgga aaacggtgta acaagggtga
     2461 acactatccc atatcaccag ctcaccgtct ttcattgcca tacgaaattc cggatgagca
     2521 ttcatcaggc gggcaagaat gtgaataaag gccggataaa acttgtgctt atttttcttt
     2581 acggtcttta aaaaggccgt aatatccagc tgaacggtct ggttataggt acattgagca
     2641 actgactgaa atgcctcaaa atgttcttta cgatgccatt gggatatatc aacggtggta
     2701 tatccagtga tttttttctc cattttagct tccttagctc ctgaaaatct cgataactca
     2761 aaaaatacgc ccggtagtga tcttatttca ttatggtgaa agttggaacc tct
//
