LOCUS       sequence-106568-        5735 bp    DNA     circular SYN 14-APR-2025
DEFINITION  This plasmid is designed as a bacterial oxidative stress sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5735)
  TITLE     Salis Lab Plasmids
REFERENCE   2  (bases 1 to 5735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: yqhD-RFP
FEATURES             Location/Qualifiers
     source          1..5735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(130..147)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(264..809)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(344..363)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     885..904
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     CDS             1407..2078
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="SSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKLK
                     VTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVT
                     VTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMRL
                     KLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHSTG
                     A"
     promoter        2153..2187
                     /label=BBa_J23102
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 0.86"
     terminator      3022..3108
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(3191..3208)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      3361..3447
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3539..3566
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     promoter        3585..3676
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3677..3745,3746..4537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3895..3914)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4579..5034
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4666..4685)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tagcggagtg tatactggct tactatgttg gcactgatga gggtgtcagt gaagtgcttc
       61 atgtggcagg agaaaaaagg ctgcaccggt gcgtcagcag aatatgtgat acaggatata
      121 ttccgcttcc tcgctcactg actcgctacg ctcggtcgtt cgactgcggc gagcggaaat
      181 ggcttacgaa cggggcggag atttcctgga agatgccagg aagatactta acagggaagt
      241 gagagggccg cggcaaagcc gtttttccat aggctccgcc cccctgacaa gcatcacgaa
      301 atctgacgct caaatcagtg gtggcgaaac ccgacaggac tataaagata ccaggcgttt
      361 ccccctggcg gctccctcgt gcgctctcct gttcctgcct ttcggtttac cggtgtcatt
      421 ccgctgttat ggccgcgttt gtctcattcc acgcctgaca ctcagttccg ggtaggcagt
      481 tcgctccaag ctggactgta tgcacgaacc ccccgttcag tccgaccgct gcgccttatc
      541 cggtaactat cgtcttgagt ccaacccgga aagacatgca aaagcaccac tggcagcagc
      601 cactggtaat tgatttagag gagttagtct tgaagtcatg cgccggttaa ggctaaactg
      661 aaaggacaag ttttggtgac tgcgctcctc caagccagtt acctcggttc aaagagttgg
      721 tagctcagag aaccttcgaa aaaccgccct gcaaggcggt tttttcgttt tcagagcaag
      781 agattacgcg cagaccaaaa cgatctcaag aagatcatct tattaatcag ataaaatatt
      841 tctaggatca tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata
      901 taggcgccag caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag
      961 aggatctgct catgtttgac agcttatcat cgatgcataa tgtgcctgtc aaatggacga
     1021 agcagggatt ctgcaaaccc tatgctactc cctcgagccg gatccagcgt aatttcttag
     1081 gaataatgcg gcaatttgat tgtgcgcaat tttgtagcat ttctccagca ctctggagaa
     1141 ataggcaaga cattggcaga aatgagcatt gagagccagg gcgctggcga tcacaatgaa
     1201 aaacatcagg cagatcgttc tctgccctca tattggccca gcaaagggag caagtaatga
     1261 acaactttaa tctgcacacc ccaacccgca ttctgtttgg taaaggcgca atcgctggtt
     1321 tacgcgaaca aattcctcac gatgctcgcg tattgattac ctacggcggc ggcagcgtga
     1381 aaaaaaccgg gaattctaaa aatctgagct ctgaagacgt tatcaaagag ttcatgcgtt
     1441 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
     1501 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
     1561 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
     1621 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
     1681 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
     1741 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
     1801 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
     1861 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
     1921 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
     1981 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
     2041 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata aggccggccg tgctagtgta
     2101 gaaatagccg ccagttccgc tggcggcatt ttaactttct ttagtactag tattgacagc
     2161 tagctcagtc ctaggtactg tgctagcacc ccaatcgacg tcatactaga atcgggtcac
     2221 ggaggtacaa taataatgcg taaactcgag gaacttttca cgggggtcgt cccaattctg
     2281 gtagaattag acggtgatgt aaacgggcac aagtttagcg tcagcggaga gggcgagggg
     2341 gacgccacct acggtaaact tacccttaag tttatttgca cgacgggcaa attacccgtt
     2401 ccgtggccaa cgctcgtcac tactttcggc tacggcgttc agtgcttcgc gagatatccg
     2461 gatcacatga agcagcacga tttcttcaag tcggccatgc cagagggtta cgttcaggag
     2521 agaactatat ttttcaagga cgacggcaat tacaagacac gcgccgaagt caagttcgaa
     2581 ggcgataccc tggttaaccg tatcgagtta aaaggtatcg attttaaaga agacggaaac
     2641 attctcggtc acaaactcga atacaactat aacagccata atgtctacat tatggctgac
     2701 aaacagaaga acggcattaa ggttaacttc aaaattcgcc acaacatcga agacggtagc
     2761 gtccaactag cagaccatta tcaacaaaat actcctatcg gcgacggccc agtcctttta
     2821 ccagacaacc attaccttag cacacaaagc gcccttagca aagatcccaa cgagaagcgc
     2881 gaccacatgg tccttctcga atttgtcaca gcggcaggga tcacccacgg catggacgaa
     2941 ctctacaaat aataattaat taaaaaaaaa aaccccgccc ctgacagggc ggggtttttt
     3001 tttctaatat gcggccgcca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt
     3061 cgttttatct gttgtttgtc ggtgaacgct ctcctgagta ggacaaatcc gccgccctag
     3121 agcatgcgcg ttcggctgcg gcgagcggta tcaggcttgg ctgttttggc ggatgagaga
     3181 agattttcag cctgatacag attaaatcag aacgcagaag cggtctgata aaacagaatt
     3241 tgcctggcgg cagtagcgcg gtggtcccac ctgaccccat gccgaactca gaagtgaaac
     3301 gccgtagcgc cgatggtagt gtggggtctc cccatgcgag agtagggaac tgccaggcat
     3361 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
     3421 gtgaacgctc tcctgagtag gacaaatccg ccgggagcgg atttgaacgt tgcgaagcaa
     3481 cggcccggag ggtggcgggc aggacgcccg ccataaactg ccaggcatca aattaagcag
     3541 aaggccatcc tgacggatgg cctttttgcg tttctacaaa ctcttttgtt tatttttcta
     3601 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     3661 ttgaaaaagg aagactatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     3721 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     3781 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     3841 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     3901 tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     3961 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     4021 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     4081 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     4141 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     4201 gcgtgacacc acgatgcctg cagcaatggc aacaacgttg cgcaaactat taactggcga
     4261 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     4321 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     4381 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     4441 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     4501 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     4561 tatactttag attgatttac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     4621 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     4681 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     4741 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg
     4801 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     4861 ccacgttctt taatagtgga ctcttgttcc aaacttgaac aacactcaac cctatctcgg
     4921 gctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc
     4981 tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgtttaca atttaaaagg
     5041 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     5101 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     5161 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     5221 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     5281 ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     5341 ccgcctacat acctcgctct gctaatcctg ttaccagtca ggcatttgag aagcacacgg
     5401 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
     5461 aacgaccctg ccctgaaccg acgaccgggt cgaatttgct ttcgaatttc tgccattcat
     5521 ccgcttatta tcacttattc aggcgtagca ccaggcgttt aagggcacca ataactgcct
     5581 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     5641 ctgccgacat ggaagccatc acagacggca tgatgaacct gaatcgccag cggcatcagc
     5701 accttgtcgc cttgcgtata atatttgccc atggc
//
