LOCUS       sequence-106775-        1031 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  t1 gRNA (ATGAGAATCAAGGCGGTCGA).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1031)
  AUTHORS   Moore R, Spinhirne A, Lai MJ, Preisser S, Li Y, Kang T, Bleris L
  TITLE     CRISPR-based self-cleaving mechanism for controllable gene delivery 
            in human cells.
  JOURNAL   Nucleic Acids Res. 2015 Jan 30;43(2):1297-303. doi: 
            10.1093/nar/gku1326. Epub 2014 Dec 18.
  PUBMED    25527740
REFERENCE   2  (bases 1 to 1031)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku1326"; journalName: "Nucleic Acids Res"; date: 
            "2015-01-30- 30"; volume: "43"; issue: "2"; pages: "1297-303"
COMMENT     Sequence Label: pCas9–mKate2ps–T1gRNA
FEATURES             Location/Qualifiers
     source          1..1031
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(52..105)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(109..129)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cttcatgtgc atgttctcct taatcagctc gctcaccatg ggccctccgg aagggccggg
       61 attctcctcc acgtcaccgc atgttagaag acttcctctg ccctcagcca ccttcctctt
      121 cttcttgggg tcagccctgc tgtctccacc gagctgagag aggtcgattc ttgtttcata
      181 gagccccgta attgactgat gaatcagtgt ggcgtccagg acctcctttg tagaggtgta
      241 ccgctttctg tctatggtgg tgtcgaagta cttgaaggct gcaggcgcgc ccaagttggt
      301 cagagtaaac aagtggataa tgttttctgc ctgctccctg atgggcttat ccctgtgctt
      361 attgtaagca gaaagcacct tatcgaggtt agcgtcggcg aggatcactc ttttggagaa
      421 ttcgcttatt tgctcgatga tctcatcaag gtagtgtttg tgttgttcca cgaacagctg
      481 cttctgctca ttatcttcgg gagacccttt gagcttttca tagtggctgg ccagatacaa
      541 gaaattaacg tatttagagg gcagtgccag ctcgttacct ttctgcagct cgcccgcact
      601 agcgagcatt cgtttccggc cgttttcaag ctcaaagaga gagtacttgg gaagcttaat
      661 gatgaggtct tttttgacct ctttatatcc tttcgcctcg agaaagtcga tggggttttt
      721 ttcgaagctt gatcgctcca tgattgtgat gcccagcagt tccttgacgc ttttgagttt
      781 tttagacttc cctttctcca ctttggccac aaccagtaca ctgtaagcga ctgtaggaga
      841 atcgaatccg ccgtatttct tggggtccca atcttttttg cgtgcgatca gcttgtcgct
      901 gttccttttc gggaggatac tttccttgga gaagcctccg gtctgtactt cggtcttttt
      961 aacgatgttc acctgcggca tggacaggac cttccggact gtcgcgaaat ccctaccctt
     1021 gtcccacacg a
//
