LOCUS       sequence-106794-         887 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 488, Emission: 507.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mEGFP-H4-23
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    357..409
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      417..426
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(423..425,426..428,429..887)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(468..489)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(729..748)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcagagctg gtttagtgaa ccgtcagatc cgctagccgc caccatgtct ggccgcggca
       61 aaggcgggaa gggtcttggc aaaggcggcg ctaagcgcca ccgtaaagta ctgcgcgaca
      121 atatccaggg catcaccaag ccggccatcc ggcgccttgc tcgccgcggc ggcgtgaagc
      181 gcatctccgg cctcatctac gaggagactc gcggggtgct gaaggtgttc ctggagaacg
      241 tgatccggga cgccgtgacc tatacagagc acgccaagcg caagacggtc accgccatgg
      301 atgtggtcta cgcgctcaag cgccagggcc gcaccctcta cggtttcggt ggggatctcg
      361 agctcaagct tcgaattctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca
      421 ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg gtcgagctgg
      481 acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc gatgccacct
      541 acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg ccctggccca
      601 ccctcgtgac caccctgacc tacggcgtgc agtgcttcag ccgctacccc gaccacatga
      661 agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag cgcaccatct
      721 tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc
      781 tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac atcctggggc
      841 acaagctgga gtacaactac aacagccaca acgtctatat catggcc
//
