LOCUS       sequence-106918-         726 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  splicing reporter (with intron) with elements to shorten the 
            half-life of the luciferase protein as well as the luciferase mRNA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 726)
  AUTHORS   Younis I, Berg M, Kaida D, Dittmar K, Wang C, Dreyfuss G
  TITLE     Rapid-response splicing reporter screens identify differential 
            regulators of constitutive and alternative splicing.
  JOURNAL   Mol Cell Biol. 2010 Apr;30(7):1718-28. doi: 10.1128/MCB.01301-09. 
            Epub 2010 Feb 1.
  PUBMED    20123975
REFERENCE   2  (bases 1 to 726)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01301-09"; journalName: "Mol Cell Biol"; date: 
            "2010-04"; volume: "30"; issue: "7"; pages: "1718-28"
COMMENT     Sequence Label: CMV-LUC2CP/intron/ARE
FEATURES             Location/Qualifiers
     source          1..726
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             56..103
                     /codon_start=1
                     /product="non-ORF yeast peptide conferring 
                     ubiquitin-dependent degradation"
                     /label=hCL1
                     /note="human codon-optimized"
                     /translation="ACKNWFSSLSHFVIHL"
     CDS             107..226
                     /codon_start=1
                     /product="PEST degradation sequence from mouse ornithine 
                     decarboxylase"
                     /label=hPEST
                     /note="human codon-optimized"
                     /translation="SHGFPPEVEEQAAGTLPMSCAQESGMDRHPAACASARINV"
     polyA_signal    299..420
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(310..329)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     364..383
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(637..657)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gatccgcgag attctcatta aggccaagaa gggcggcaag atcgccgtga attctgcttg
       61 caagaactgg ttcagtagct taagccactt tgtgatccac cttaacagcc acggcttccc
      121 tcccgaggtg gaggagcagg ccgccggcac cctgcccatg agctgcgccc aggagagcgg
      181 catggataga caccctgctg cttgcgccag cgccaggatc aacgtctaac tgcagtctag
      241 atttatttat ttatttattt ctagagtcgg ggcggccggc cgcttcgagc agacatgata
      301 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      361 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      421 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
      481 taaagcaagt aaaacctcta caaatgtggt aaaatcgata aggatccgtt tgcgtattgg
      541 gcgctcttcc gctgatctgc gcagcaccat ggcctgaaat aacctctgaa agaggaactt
      601 ggttagctac cttctgaggc ggaaagaacc agctgtggaa tgtgtgtcag ttagggtgtg
      661 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
      721 caacca
//
