LOCUS       sequence-106956-         705 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag Mis12 for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 705)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 705)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pIC65
FEATURES             Location/Qualifiers
     source          1..705
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             80..124
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      121 cagcggaggt ggaggtacta gtctcgagat gtctgtggat ccaatgacct acgaggccca
      181 gttctttggc ttcacgccac aaacgtgcat gcttcggatc tacattgcat ttcaagacta
      241 cctatttgaa gtgatgcagg ccgttgaaca ggttattctg aagaagctgg atggcatccc
      301 agactgtgac attagcccag tgcagattcg caaatgcaca gagaagtttc tttgcttcat
      361 gaaaggacat tttgataacc tttttagcaa aatggagcaa ctgtttttgc agctgatttt
      421 acgtattccc tcaaacatct tgcttcctga agataaatgt aaggagacac cttatagtga
      481 ggaagatttt cagcatctcc agaaagaaat tgaacagtta caggagaagt acaagactga
      541 attatgtact aagcaggccc ttcttgcaga attagaagag caaaaaattg ttcaggccaa
      601 actcaaacag acgttgactt tctttgatga gcttcataat gttggcagag atcatgggac
      661 tagtgatttt agggagagtt tagtatccct ggttcagaac tccag
//
