LOCUS       sequence-106958-         871 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression of HSPB5 (small HSP) tagged with V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Vos MJ, Zijlstra MP, Kanon B, van Waarde-Verhagen MA, Brunt ER, 
            Oosterveld-Hut HM, Carra S, Sibon OC, Kampinga HH
  TITLE     HSPB7 is the most potent polyQ aggregation suppressor within the 
            HSPB family of molecular chaperones.
  JOURNAL   Hum Mol Genet. 2010 Dec 1;19(23):4677-93. doi: 10.1093/hmg/ddq398. 
            Epub 2010 Sep 15.
  PUBMED    20843828
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/hmg/ddq398"; journalName: "Hum Mol Genet"; date: 
            "2010-12-1- 1"; volume: "19"; issue: "23"; pages: "4677-93"
COMMENT     Sequence Label: Frt-V5-hspB5
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(677..718)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     primer_bind     complement(821..845)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 actcgatttt ggcgcgtaat ttgggcctgc ccttagcatt aataagcttc agcactagtc
       61 acaagacttt cattcactgg tggggaaact ttcttgtttt aaaaaaatgc aattcaagaa
      121 agggcatcta tttcttgggg gctgcggtga cagcaggctt ctcttcacgg gtgatgggaa
      181 tggtgcgctc agggccagag acctgtttcc ttggtccatt cacagtgagg accccatcag
      241 atgacaggga tgaagtaatg gtgagagggt ctacatcagc tgggatccgg tatttcctgt
      301 ggaactccct ggagatgaaa ccatgttcat cctggcgctc ttcatgtttt ccatgcacct
      361 caatcacatc tcccaacacc ttaactttga gttcctctgg ggagaagtgc ttcacatcca
      421 ggttgacaga gaacctgtcc ttctccaggc gcatctctga gagtccagtg tcaaaccagc
      481 tgggtgcccg caggaaggag ggtggccgaa ggtagaaggg actcagggaa gtagacgtcg
      541 ggaaaagatc agactccaac aggtgctctc cgaagaactg gtcaaagagg cggctggggg
      601 agtggaaagg aaagaagggg cggcggatcc aggggtggtg gatggcgatg tccatggttc
      661 tcgaggtttt ggatcccgta gaatcgagac cgaggagagg gttagggata ggcttaccca
      721 tggtgaattc aagcttaagt ttaaacgcta gagtccggag gctggatcgg tcccggtgtc
      781 ttctatggag gtcaaaacag cgtggatggc gtctccaggc gatctgacgg ttcactaaac
      841 gagctcgtcg acgatctcta tcactgatag g
//
