LOCUS       sequence-106988-        6886 bp    DNA     circular SYN 15-APR-2025
DEFINITION  expresses crtEBI genes for neurosporene production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6886)
  AUTHORS   Farasat I, Kushwaha M, Collens J, Easterbrook M, Guido M, Salis HM
  TITLE     Efficient search, mapping, and optimization of multi-protein genetic
            systems in diverse bacteria.
  JOURNAL   Mol Syst Biol. 2014 Jun 21;10:731. doi: 10.15252/msb.20134955.
  PUBMED    24952589
REFERENCE   2  (bases 1 to 6886)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Syst 
            Biol."; date: "2014-06-21"; volume: "10:731. doi"; pages: " 
            10.15252/msb.20134955"
COMMENT     Sequence Label: pBad_crtEBI_19
FEATURES             Location/Qualifiers
     source          1..6886
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(89..967)
                     /codon_start=1
                     /gene="araC"
                     /product="L-arabinose regulatory protein"
                     /label=araC
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKGY
                     ILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGRHPEAREWYHQWVYFRPRAYW
                     HEWLNWPSIFANTGFFRPDEAHQPHFSDLFGQIINAGQGEGRYSELLAINLLEQLLLRR
                     MEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQLGIS
                     VLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEFRAGCE
                     EKVNDVAVKLS"
     promoter        994..1278
                     /gene="araBAD"
                     /label=araBAD promoter
                     /note="promoter of the L-arabinose operon of E. coli; the 
                     araC regulatory gene is transcribed in the opposite 
                     direction (Guzman et al., 1995)"
     primer_bind     1201..1220
                     /label=pBAD Forward
                     /note="For vectors with E. coli araBAD promoter, forward 
                     primer"
     RBS             3333..3341
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     terminator      complement(5052..5079)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(5095..5166)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             5279..5938
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(5345..5364)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      5959..6053
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     rep_origin      6142..6730
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6631..6650
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cataatgtgc ctgtcaaatg gacgaagcag ggattctgca aaccctatgc tactccctcg
       61 agccgtcaat tgtctgattc gttaccaatt atgacaactt gacggctaca tcattcactt
      121 tttcttcaca accggcacgg aactcgctcg ggctggcccc ggtgcatttt ttaaataccc
      181 gcgagaaata gagttgatcg tcaaaaccaa cattgcgacc gacggtggcg ataggcatcc
      241 gggtggtgct caaaagcagc ttcgcctggc tgatacgttg gtcctcgcgc cagcttaaga
      301 cgctaatccc taactgctgg cggaaaagat gtgacagacg cgacggcgac aagcaaacat
      361 gctgtgcgac gctggcgata tcaaaattgc tgtctgccag gtgatcgctg atgtactgac
      421 aagcctcgcg tacccgatta tccatcggtg gatggagcga ctcgttaatc gcttccatgc
      481 gccgcagtaa caattgctca agcagattta tcgccagcag ctccgaatag cgcccttccc
      541 cttgcccggc gttaatgatt tgcccaaaca ggtcgctgaa atgcggctgg tgcgcttcat
      601 ccgggcgaaa gaaccccgta ttggcaaata ttgacggcca gttaagccat tcatgccagt
      661 aggcgcgcgg acgaaagtaa acccactggt gataccattc gcgagcctcc ggatgacgac
      721 cgtagtgatg aatctctcct ggcgggaaca gcaaaatatc acccggtcgg caaacaaatt
      781 ctcgtccctg atttttcacc accccctgac cgcgaatggt gagattgaga atataacctt
      841 tcattcccag cggtcggtcg ataaaaaaat cgagataacc gttggcctca atcggcgtta
      901 aacccgccac cagatgggca ttaaacgagt atcccggcag caggggatca ttttgcgctt
      961 cagccatact tttcatactc ccgccattca gagaagaaac caattgtcca tattgcatca
     1021 gacattgccg tcactgcgtc ttttactggc tcttctcgct aaccaaaccg gtaaccccgc
     1081 ttattaaaag cattctgtaa caaagcggga ccaaagccat gacaaaaacg cgtaacaaaa
     1141 gtgtctataa tcacggcaga aaagtccaca ttgattattt gcacggcgtc acactttgct
     1201 atgccatagc atttttatcc ataagattag cggatcttac ctgacgcttt ttatcgcaac
     1261 tctctactgt ttctccatac ccgtgaattc aaccaatata ccaaataaag agttgcggcg
     1321 gtcaaggatg gcattcgaac aacgtattga agctgctatg gctgctgcta ttgcacgtgg
     1381 ccagggtagc gaagctccgt ccaaactggc aactgcgctg gactacgctg tcacccctgg
     1441 tggtgcgcgc attcgtccga ctctgctgct gtctgtcgcc accgcttgcg gcgatgaccg
     1501 tcctgccctg agcgacgcgg ctgcggttgc cctggaactg atccattgcg cttctctggt
     1561 gcatgacgac ctgccgtgct tcgacgacgc tgaaatccgt cgtggtaagc caaccgttca
     1621 tcgtgcatac agcgaaccgc tggctatcct gactggtgat tccctgatcg ttatgggttt
     1681 cgaagttctg gcgcgtgcgg cggctgatca gccacaacgt gccctgcaac tggtaacggc
     1741 tctggcggta cgcactggta tgccgatggg catctgcgct ggtcagggtt gggaaagcga
     1801 atctcagatc aacctgtctg cataccaccg tgccaaaact ggtgccctgt tcatcgcggc
     1861 tactcagatg ggcgcaatcg cagcgggtta cgaggcggaa ccgtgggaag aactgggtgc
     1921 gcgtatcggc gaagcgttcc aggttgcaga tgatctgcgt gacgcgctgt gcgatgccga
     1981 aaccctgggt aagccggcag gtcaggacga aatccatgca cgtccgaacg ctgttcgcga
     2041 gtatggtgta gaaggcgcgg ctaaacgcct gaaagacatc ctgggtggcg cgatcgcatc
     2101 catcccgtcc tgtccaggtg aagcaatgct ggcagagatg gttcgtcgct acgccgagaa
     2161 aatcgttcca gctcaagttg ctgcacgcgt gtaatctaga acgtacacac acaattatac
     2221 taaggggatc cgaaatgatt gcttctgctg atctggatgc gtgtcgtgaa atgattcgta
     2281 ccggctccta tagctttcat gccgcttctc gtctgctgcc ggaacgcgta cgtgcgccga
     2341 gcctggcgct gtacgccttc tgccgtgtag ctgacgatgc tgtggatgaa gctgttaacg
     2401 atggccagcg tgaagaagat gctgaagtga aacgccgcgc agtactgagc ctgcgtgatc
     2461 gtctggatct ggtgtatggc ggccgtccgc gtaacgctcc tgctgaccgc gcctttgcgg
     2521 cggtcgtcga agaatttgaa atgccgcgtg ctctgccgga agcgctgctg gaaggtctgg
     2581 cgtgggatgc ggtaggccgt tcctacgact ccttcagcgg tgttctggat tactctgcgc
     2641 gtgttgccgc agccgttggc gcgatgatgt gtgttctgat gcgcgttcgc gatcctgatg
     2701 tcctggctcg tgcgtgcgat ctgggtctgg cgatgcaact gaccaacatc gctcgtgacg
     2761 ttggcactga cgcccgttct ggtcgcatct acctgccgcg cgactggatg gaagaggaag
     2821 gcctgccggt ggaagagttc ctggcacgcc cggtggttga tgatcgcatc cgtgcagtca
     2881 ctcaccgcct gctgcgcgct gcggaccgtc tgtacctgcg ttctgaggcc ggtgtgtgtg
     2941 gcctgccgct ggcttgtcgt ccgggcattt acgcggcacg tcacatctat gctggtattg
     3001 gtgatgaaat cgcgcgtaac ggttatgatt ctgttacccg tcgcgctttc accactcgtc
     3061 gccagaaact ggtttggctg ggtctgtccg ctactcgcgc tgctctgagc ccattcggtc
     3121 caggttgtgc aaccctgcac gccgctccgg aaccagaagt cgcattcctg gttaacgcgg
     3181 cagcacgcgc tcgtcctcag cgtggccgta gcgaagctct gattagcgtt ctggctcaac
     3241 tggaggctca ggatcgtcag atcagccgcc agcgtctggg taaccgtgcg aacccgatct
     3301 aactcgaagc ttaaacccaa caaattagac tataaggagg ttaataatgc catctatttc
     3361 tccagcctct gacgctgatc gtgctctggt tatcggctct ggcctgggcg gcctggccgc
     3421 tgctatgcgt ctgggtgcaa aaggttggcg tgttaccgta atcgataaac tggacgttcc
     3481 gggtggccgt ggctccagca tcacgcagga gggccatcgt tttgacctgg gcccgaccat
     3541 tgttactgta cctcaaagcc tgcgtgacct gtggaaaact tgcggtcgcg acttcgacgc
     3601 agatgtggag ctgaaaccga ttgacccatt ctacgaagtt cgttggccag atggctctca
     3661 ctttaccgtt cgtcagtcta ctgaagctat gaaagctgaa gtagcacgcc tgtctccagg
     3721 cgatgttgcg ggctacgaga aattcctgaa ggattccgag aaacgttatt ggttcggtta
     3781 tgaggacctg ggtcgtcgct ccatgcacaa actgtgggat ctgatcaaag tcctgccgac
     3841 cttcggcatg atgcgtgctg atcgttccgt ctaccagcac gcggccctgc gcgtcaagga
     3901 tgaacgcctg cgcatggcgc tgtccttcca tcctctgttc attggtggtg atccgttcaa
     3961 tgtgaccagc atgtacatcc tggtatctca actggagaaa gaatttggtg tacattacgc
     4021 gatcggcggt gtggccgcca tcgcagccgc gatggcgaaa gtgattgaag gtcagggcgg
     4081 ctccttccgc atgaacactg aagtggacga aatcctggtt gaaaaaggca ccgctaccgg
     4141 tgttcgcctg gcgtctggcg aagtactgcg cgctggtctg gttgtaagca atgcggacgc
     4201 aggccatacc tacatgcgtc tgctgcgcaa tcaccctcgc cgtcgttgga ctgacgccca
     4261 cgttaaatct cgccgctgga gcatgggcct gtttgtttgg tactttggca ccaaaggtac
     4321 taaaggcatg tggccggatg tgggtcatca caccatcgtg aacgctccgc gttacaaagg
     4381 cctggttgag gacatcttcc tgaaaggtaa actggccaaa gacatgtccc tgtacatcca
     4441 tcgtccatct atcactgatc cgaccgttgc gccagaaggc gacgatacct tctacgcgct
     4501 gtccccagtg ccgcacctga aacaggcaca gccggtagac tggcaggcgg ttgcggagcc
     4561 gtaccgcgaa agcgtcctgg aagtcctgga acaatccatg ccgggcattg gtgaacgcat
     4621 tggtccaagc ctggtgttca cgccagaaac tttccgtgat cgttacctgt ccccgtgggg
     4681 tgcaggcttc tctatcgaac cgcgcatcct gcaatctgct tggttccgtc cgcataatat
     4741 ctctgaagaa gtcgcaaatc tgtttctggt tggtgcgggc acccacccag gtgccggtgt
     4801 accgggtgtt attggctctg cggaggtcat ggcgaaactg gctccggatg cgccgcgtgc
     4861 gcgccgtgaa gctgaaccgg ctgagcgcct ggcagcagaa taaccataac acacacacac
     4921 acaaggtcgt cacagcaccg gtgcttaata aagatcttcg acgtctgctg tgtaaaaaaa
     4981 aaccccgccc ctgacagggc ggggtttttt ttcgcggttg tgaatgccat atggctgtgc
     5041 tgtggtataa acgcagaaag gcccacccga aggtgagcca gtgtgactct agtagagagc
     5101 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
     5161 tatttgatgc ctggcctagg ttcacgtaag aggttccaac tttcaccata atgaaataag
     5221 atcactaccg ggcgtatttt ttgagttatc gagattttca ggagctaagg aagctaaaat
     5281 ggagaaaaaa atcactggat ataccaccgt tgatatatcc caatggcatc gtaaagaaca
     5341 ttttgaggca tttcagtcag ttgctcaatg tacctataac cagaccgttc agctggatat
     5401 tacggccttt ttaaagaccg taaagaaaaa taagcacaag ttttatccgg cctttattca
     5461 cattcttgcc cgcctgatga atgctcatcc ggaatttcgt atggcaatga aagacggtga
     5521 gctggtgata tgggatagtg ttcacccttg ttacaccgtt ttccatgagc aaactgaaac
     5581 gttttcatcg ctctggagtg aataccacga cgatttccgg cagtttctac acatatattc
     5641 gcaagatgtg gcgtgttacg gtgaaaacct ggcctatttc cctaaagggt ttattgagaa
     5701 tatgtttttc gtctcagcca atccctgggt gagtttcacc agttttgatt taaacgtggc
     5761 caatatggac aacttcttcg cccccgtttt caccatgggc aaatattata cgcaaggcga
     5821 caaggtgctg atgccgctgg cgattcaggt tcatcatgcc gtttgtgatg gcttccatgt
     5881 cggcagaatg cttaatgaat tacaacagta ctgcgatgag tggcagggcg gggcgtaatt
     5941 tgatatcggt accgcttgga ctcctgttga tagatccagt aatgacctca gaactccatc
     6001 tggatttgtt cagaacgctc ggttgccgcc gggcgttttt tattggtgag aatccaagca
     6061 cccgggcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6121 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6181 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6241 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta
     6301 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6361 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6421 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6481 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6541 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6601 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6661 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6721 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6781 tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt
     6841 tgagtgagct gatacctgga tcctgataaa tgatttctgc agaaaa
//
