LOCUS       sequence-107023-         692 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Retroviral expression of EGFP-TEV-S-tag HP1 alpha for protein 
            localization and affinity purification (LAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 692)
  TITLE     Cheeseman Lab Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pKG204
FEATURES             Location/Qualifiers
     source          1..692
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             47..67
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             80..124
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
ORIGIN
        1 gtccggaggg gatcggtgga gctccaccgg tggcggccgc tctagagaga atctttattt
       61 tcagggcgcc gccaaattca aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga
      121 cagcggaggt ggaggtacta gtctcgagat gggaaagaaa accaagcgga cagctgacag
      181 ttcttcttca gaggatgagg aggagtatgt tgtggagaag gtgctagaca ggcgcgtggt
      241 taagggacaa gtggaatatc tactgaagtg gaaaggcttt tctgaggagc acaatacttg
      301 ggaacctgag aaaaacttgg attgccctga gctaatttct gaatttatga aaaagtataa
      361 gaagatgaag gagggtgaaa ataataaacc cagggagaag tcagaaagta acaagaggaa
      421 atccaatttc tcaaacagtg ccgatgacat caaatctaaa aaaaagagag agcagagcaa
      481 tgatatcgct cggggctttg agagaggact ggaaccagaa aagatcattg gggcaacaga
      541 ttcctgtggt gatttaatgt tcctaatgaa atggaaagac acagatgaag ctgacctgat
      601 tcttgcaaaa gaagctaatg tgaaatgtcc acaaattgtg atagcatttt atgaagagag
      661 actgacatgg catgcatatc ctgaggatgc gg
//
