LOCUS       sequence-107038-         824 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Plasmid for run-off in vitro transcription of Saccharomyces 
            arboricola KBS RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  AUTHORS   Dalby AB, Goodrich KJ, Pfingsten JS, Cech TR
  TITLE     RNA recognition by the DNA end-binding Ku heterodimer.
  JOURNAL   RNA. 2013 Jun;19(6):841-51. doi: 10.1261/rna.038703.113. Epub 2013 
            Apr 22.
  PUBMED    23610127
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.038703.113"; journalName: "RNA"; date: "2013-06"; 
            volume: "19"; issue: "6"; pages: "841-51"
COMMENT     Sequence Label: pAD04 S. arboricola telomerase RNA KBS
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(113..129)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(113..129)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(126..148)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    137..153
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(161..167,168..185,186..191))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    206..227
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(344..361)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(595..614)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttttttcac ttgtagatcg ctaaaatctg agcttgagaa atgaagctcc ttttttcttc
       61 atccggatcc tctagagtcg acctgcaggc atgcaagctt ggcgtaatca tggtcatagc
      121 tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca
      181 taaagtgtaa agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct
      241 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac
      301 gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc
      361 tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt
      421 tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg
      481 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg
      541 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat
      601 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta
      661 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct
      721 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc
      781 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tctt
//
