LOCUS       sequence-107044-         830 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Plasmid for run-off in vitro transcription of Saccharomyces 
            paradoxus KBS RNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Dalby AB, Goodrich KJ, Pfingsten JS, Cech TR
  TITLE     RNA recognition by the DNA end-binding Ku heterodimer.
  JOURNAL   RNA. 2013 Jun;19(6):841-51. doi: 10.1261/rna.038703.113. Epub 2013 
            Apr 22.
  PUBMED    23610127
REFERENCE   2  (bases 1 to 830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.038703.113"; journalName: "RNA"; date: "2013-06"; 
            volume: "19"; issue: "6"; pages: "841-51"
COMMENT     Sequence Label: pAD02 S. paradoxus telomerase RNA KBS
FEATURES             Location/Qualifiers
     source          1..830
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(134..150)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(134..150)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(147..169)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    158..174
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(182..188,189..206,207..212))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    227..248
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(365..382)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(616..635)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 acgactcact ataggagcgt tatttttcta cttatagatg gctaaaatct gagtttgaaa
       61 gatggcgctc cttttttctt catccggatc ctctagagtc gacctgcagg catgcaagct
      121 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac
      181 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac
      241 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc
      301 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg
      361 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc
      421 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt
      481 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc
      541 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa
      601 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc
      661 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg
      721 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc
      781 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
//
