LOCUS       sequence-107081-         935 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Yeast two-hybrid bridge vector expressing PAA-1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 935)
  AUTHORS   Wang JT, Smith J, Chen BC, Schmidt H, Rasoloson D, Paix A, Lambrus 
            BG, Calidas D, Betzig E, Seydoux G
  TITLE     Regulation of RNA granule dynamics by phosphorylation of 
            serine-rich, intrinsically disordered proteins in C. elegans.
  JOURNAL   Elife. 2014 Dec 23;4. doi: 10.7554/eLife.04591.
  PUBMED    25535836
REFERENCE   2  (bases 1 to 935)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-12-23"; volume: "4. doi"; pages: " 10.7554/eLife.04591"
COMMENT     Sequence Label: pJW3.31
FEATURES             Location/Qualifiers
     source          1..935
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..20
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     5..21
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    54..78
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gactcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc atcaccactt
       61 tgtacaagaa agctgggtcc gattgagaag tggaagatgt agagattcca atttgactag
      121 cgatatgttc gattacaagc cgagtgagtt cttggcttcc tctgaaaagt atcgaacgtc
      181 aaagtctgaa tcttttccga gcttctcgag cagtggcttc acctcggacg tcaatgtact
      241 tggggtcaag ttctttccaa ttcgcttcaa actctttgcg gcgttgaatc tgacattcgg
      301 cacgtcatcc tcgacaagat ctttcacaat tggcataatc tccttcagaa tctgctcctg
      361 agtcatcgcc tcactgagtg tattcaaaca gaacaagcat gtcattctct gcaggtagtt
      421 ggtgtccttc tgaagtttct gcatttttgg tacaatattc gtgctagccc attgtcctcc
      481 aaacttctgt gtcagttcct tcatgatgag ggtcgatgcc tcgcggattg agaacacatg
      541 gtcggtaagc cagttcaaac aaagcggaag caacttctca tcgaagaatt cttgtcccaa
      601 ctgggaagca agaagtggca tgaattgaac aattgcgagt cgaactctcc attttccatc
      661 ctcggccaat ccaacgatgg ctggcaagag ggatgtggac aattgagctg ctccgatcac
      721 ttcattgacc ttatcaagac tggaaataat attgaggcga acttctgggg tttgatcgtt
      781 aagcaactgc atgtagattg ggagaagctc agagacagtt tgttccttgc caatcagtgg
      841 agcaagtccc atgatgacac cggcgagttc cgacttgacg agctgattcc catcagtaac
      901 caattccttg gcaacgttga gcagtgagtt gcaga
//
