LOCUS       sequence-107095-        7455 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta 
            AS4(-) [lacking the insert at alternatively spliced segment 4 
            (AS4-)].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7455)
  AUTHORS   Scheiffele P, Fan J, Choih J, Fetter R, Serafini T
  TITLE     Neuroligin expressed in nonneuronal cells triggers presynaptic 
            development in contacting axons.
  JOURNAL   Cell. 2000 Jun 9. 101(6):657-69.
  PUBMED    10892652
REFERENCE   2  (bases 1 to 7455)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2000 
            Jun 9. 101(6):657-69."
COMMENT     Sequence Label: pcDNA3-Nrxn1beta AS4(-)-Fc
FEATURES             Location/Qualifiers
     source          1..7455
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(40..59)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        231..610
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        611..814
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     765..785
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     859..878
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        859..877
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3007..3025)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3008..3025)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3045..3062)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3051..3275
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3321..3749
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3408..3427)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3618..3639
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3758..3778)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3763..4093
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3944..4079
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4006..4025
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             4160..4954
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(4214..4233)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     4824..4843
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    5128..5249
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5165..5184)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5219..5238
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5298..5314)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5298..5314)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5311..5333)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5322..5338
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5346..5352,5353..5370,5371..5376))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5391..5412
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5529..5546)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5700..6288)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5780..5799)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6459..7250,7251..7319))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7082..7101
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7320..7424)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gttcccgatc ccctatggtc gactctcagt acaatctgct ctgatgccgc
       61 atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt agtgcgcgag
      121 caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga atctgcttag
      181 ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt gacattgatt
      241 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
      301 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
      361 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
      421 acgtcaatgg gtggactatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
      481 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
      541 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
      601 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
      661 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
      721 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
      781 gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag aacccactgc
      841 ttactggctt atcgaaatta atacgactca ctatagggag acccaagctt accatgtacc
      901 agaggatgct ccggtgcggc gccgatctgg gatcgcccgg gggcggcagt ggcggcggcg
      961 caggggggcg cctggccctg atctggatag tcccgctcac cctcggcggc ctcctaggag
     1021 tggcctgggg ggcatccagt ttgggagcgc accacatcca ccatttccat ggcagcagca
     1081 agcatcattc agtgcctatt gcaatctaca ggtcaccagc atccttgcga ggcggacacg
     1141 ctgggacaac atatatcttt agcaaaggtg gtggacagat tacatataag tggcctccca
     1201 atgaccgccc cagtacacga gcagacaggc tggccatcgg atttagcact gtccagaagg
     1261 aagccgtgtt ggtgcgtgtg gacagttcct caggcctggg tgactacctg gagctgcaca
     1321 tacaccaagg aaaaattgga gttaagttta atgttgggac agatgacatc gccatcgaag
     1381 agtctaatgc aatcattaat gatgggaaat accatgtagt acgtttcaca aggagtggtg
     1441 gcaatgccac gttacaggtg gacagctggc cagttatcga acgctaccct gcagggcgtc
     1501 agctcacaat cttcaatagc caagcaacca taataattgg cgggaaagag cagggccagc
     1561 ccttccaggg ccagctctct ggtctttact acaatggctt gaaagttctg aatatggcag
     1621 cagagaacga tgccaacatc gccatagtgg ggaatgtgag gctggtcggt gaagtgagat
     1681 ctgcggatcc cgagggtgag tacttagctt cagcgctcct gcctggacgc atcccggcta
     1741 tgcagcccca gtccagggca gcaaggcagg ccccgtctgc ctcttcaccc ggaggcctct
     1801 gcccgcccca ctcatgctca gggagagggt cttctggctt tttccccagg ctctgggcag
     1861 gcacaggcta ggtgccccta acccaggccc tgcacacaaa ggggcaggtg ctgggctcag
     1921 acctgccaag agccatatcc gggaggaccc tgcccctgac ctaagcccac cccaaaggcc
     1981 aaactctcca ctccctcagc tcggacacct tctctcctcc cagattccag taactcccaa
     2041 tcttctctct gcagagccca aatcttgtga caaaactcac acatgcccac cgtgcccagg
     2101 taagccagcc caggcctcgc cctccagctc aaggcgggac aggtgcccta gagtagcctg
     2161 catccaggga caggccccag ccgggtgctg acacgtccac ctccatctct tcctcagcac
     2221 ctgaactcct ggggggaccg tcagtcttcc tcttcccccc aaaacccaag gacaccctca
     2281 tgatctcccg gacccctgag gtcacatgcg tggtggtgga cgtgagccac gaagaccctg
     2341 aggtcaagtt caactggtac gtggacggcg tggaggtgca taatgccaag acaaagccgc
     2401 gggaggagca gtacaacagc acgtaccgtg tggtcagcgt cctcaccgtc ctgcaccagg
     2461 actggctgaa tggcaaggag tacaagtgca aggtctccaa caaagccctc ccagccccca
     2521 tcgagaaaac catctccaaa gccaaaggtg ggacccgtgg ggtgcgaggg ccacatggac
     2581 agaggccggc tcggcccacc ctctgccctg agagtgaccg ctgtaccaac ctctgtccct
     2641 acagggcagc cccgagaacc acaggtgtac accctgcccc catcccggga tgagctgacc
     2701 aagaaccagg tcagcctgac ctgcctggtc aaaggcttct atcccagcga catcgccgtg
     2761 gagtgggaga gcaatgggca gccggagaac aactacaaga ccacgcctcc cgtgctggac
     2821 tccgacggct ccttcttcct ctacagcaag ctcaccgtgg acaagagcag gtggcagcag
     2881 gggaacgtct tctcatgctc cgtgatgcat gaggctctgc acaaccacta cacgcagaag
     2941 agcctctccc tgtctccggg taaatgagtg cgacggccgc tcgagcatgc atctagaggg
     3001 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc
     3061 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
     3121 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
     3181 tcattctatt ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa
     3241 tagcaggcat gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg
     3301 gggctctagg gggtatcccc acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     3361 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     3421 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcggggcat
     3481 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     3541 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     3601 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     3661 ggtctattct tttgatttat aagggatttt ggggatttcg gcctattggt taaaaaatga
     3721 gctgatttaa caaaaattta acgcgaatta attctgtgga atgtgtgtca gttagggtgt
     3781 ggaaagtccc caggctcccc aggcaggcag aagtatgcaa agcatgcatc tcaattagtc
     3841 agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     3901 tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     3961 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4021 cgaggccgcc tctgcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4081 aggcttttgc aaaaagctcc cgggagcttg tatatccatt ttcggatctg atcaagagac
     4141 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc
     4201 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc
     4261 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc
     4321 cggtgccctg aatgaactgc aggacgaggc agcgcggcta tcgtggctgg ccacgacggg
     4381 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt
     4441 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc
     4501 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga
     4561 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga
     4621 tcaggatgat ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct
     4681 caaggcgcgc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc
     4741 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt
     4801 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg
     4861 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat
     4921 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc
     4981 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa
     5041 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat
     5101 ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa tggttacaaa
     5161 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     5221 ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac ctctagctag
     5281 agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt
     5341 ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc
     5401 taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc
     5461 cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     5521 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     5581 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     5641 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     5701 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     5761 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     5821 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     5881 gtggcgcttt ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     5941 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     6001 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     6061 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     6121 aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc
     6181 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     6241 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     6301 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     6361 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     6421 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     6481 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     6541 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     6601 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     6661 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     6721 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     6781 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     6841 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     6901 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     6961 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     7021 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     7081 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     7141 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     7201 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     7261 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     7321 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     7381 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     7441 gtgccacctg acgtc
//
