LOCUS       sequence-107251-         906 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  pRS423 backbone, contains an RFP flanked by BsaI sites with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 906)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 906)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV123
FEATURES             Location/Qualifiers
     source          1..906
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..21
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     6..22
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             complement(106..783)
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     RBS             790..801
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    819..835
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(843..849,850..867,868..873))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
ORIGIN
        1 cccctcgagg tcgacggtat cgataagctt gatatcgaat tcctgcagcc cccagtagag
       61 accgcctggc tctagtagcg atctacacta gcactatcag cgttattaag caccggtgga
      121 gtgacgacct tcagcacgtt cgtactgttc aacgatggtg tagtcttcgt tgtgggaggt
      181 gatgtccagt ttgatgtcgg ttttgtaagc acccggcagc tgaaccggtt ttttagccat
      241 gtaggtggtt ttaacttcag cgtcgtagtg accaccgtct ttcagtttca gacgcatttt
      301 gatttcacct ttcagagcac cgtcttccgg gtacatacgt tcggtggaag cttcccaacc
      361 catggttttt ttctgcataa ccggaccgtc ggacgggaag ttggtaccac gcagtttaac
      421 tttgtagatg aactcaccgt cttgcaggga ggagtcctgg gtaacggtaa caacaccacc
      481 gtcttcgaag ttcataacac gttcccattt gaaaccttcc gggaaggaca gtttcaggta
      541 gtccgggatg tcagccgggt gtttaacgta agctttggaa ccgtactgga actgcgggga
      601 caggatgtcc caagcgaacg gcagcggacc acctttggta actttcagtt tagcggtctg
      661 ggtaccttcg tacggacgac cttcaccttc accttcgatt tcgaactcgt gaccgttaac
      721 ggaaccttcc atacgaactt tgaaacgcat gaactctttg ataacgtctt cggaggaagc
      781 catctagtat ttctcctctt tctctagtat gtgtgaaatt gttatccgct cacaattcca
      841 cacaacatac gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa
      901 ctcaca
//
