LOCUS       sequence-107379-         624 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  sgRNA with 1x MS2, Pol II promoter with ribozyme-gRNA-ribozyme 
            design for yeast cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 624)
  AUTHORS   Zalatan JG, Lee ME, Almeida R, Gilbert LA, Whitehead EH, La Russa M,
            Tsai JC, Weissman JS, Dueber JE, Qi LS, Lim WA
  TITLE     Engineering Complex Synthetic Transcriptional Programs with CRISPR 
            RNA Scaffolds.
  JOURNAL   Cell. 2014 Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052.
  PUBMED    25533786
REFERENCE   2  (bases 1 to 624)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2014 
            Dec 18. pii: S0092-8674(14)01570-0. doi: 
            10.1016/j.cell.2014.11.052."
COMMENT     Sequence Label: pJZC625
FEATURES             Location/Qualifiers
     source          1..624
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           141..177
                     /product="self-cleaving hammerhead ribozyme (Ferre-D'Amare 
                     and Scott, 2010)"
                     /label=hammerhead ribozyme
                     /ncRNA_class="hammerhead_ribozyme"
     misc_RNA        198..273
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_RNA        278..296
                     /label=MS2 stem loop
                     /note="stem loop that binds the bacteriophage MS2 coat 
                     protein"
     ncRNA           303..370
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
ORIGIN
        1 tagacctgca attattaatc ttttgtttcc tcgtcattgt tctcgttccc tttcttcctt
       61 gtttcttttt ctgcacaata tttcaagcta taccaagcat acaatcaact atctcatata
      121 caatgtctct cgagaaaagt ctgatgagtc cgtgaggacg aaacgagtaa gctcgtcact
      181 tttctctatc actgatagtt ttagagctag aaatagcaag ttaaaataag gctagtccgt
      241 tatcaacttg aaaaagtggc accgagtcgg tgcgcgcaca tgaggatcac ccatgtgctt
      301 ttggccggca tggtcccagc ctcctcgctg gcgccggctg ggcaacatgc ttcggcatgg
      361 cgaatgggac gcggccgcca ccgcggtgga gctctaagca aatagctaaa ttatatacga
      421 attaatatta tgattaagtg tttacgtgag tgcgatattt ttattactat cttatacagt
      481 tgtatatact ctataaaatg agttgtctat taattaacgc gatgaatgct ttctgggttt
      541 acctctccaa caactctagt ttacttctca atacattcaa ttgtatttga tttgtcaata
      601 cttcatcatt aatcaattct atag
//
