LOCUS       sequence-107446-         827 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  TALEN array receiving vector with half-site NN RVD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  AUTHORS   Martinez RA, Stein JL, Krostag AR, Nelson AM, Marken JS, Menon V, 
            May RC, Yao Z, Kaykas A, Geschwind DH, Grimley JS
  TITLE     Genome engineering of isogenic human ES cells to model autism 
            disorders.
  JOURNAL   Nucleic Acids Res. 2015 Mar 11. pii: gkv164.
  PUBMED    25765640
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2015 Mar 11. pii: gkv164."
COMMENT     Sequence Label: AI-CN491
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(3..410)
                     /codon_start=1
                     /gene="lacZ fragment"
                     /product="LacZ-alpha fragment of beta-galactosidase"
                     /label=lacZ-alpha
                     /translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
                     QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWEDVFKGAPVSAL
                     RQACTASTAVTPPLFAIFSSCPVSSWSGGPLS"
     primer_bind     260..282
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     274..291
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     275..291
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     301..320
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        301..319
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(367..387)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(367..385)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(406..422)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(406..422)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(419..441)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    430..446
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(454..460,461..478,479..484))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    499..520
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 gagcttaggg ggcctccgct ccagctcgac accgggcagc tgctgaagat cgcgaagaga
       61 gggggagtaa cagcggtaga ggcagtgcac gcctggcgca atgcgctcac cggggccccc
      121 ttgaacacgt cttcccattc gccattcagg ctgcgcaact gttgggaagg gcgatcggtg
      181 cgggcctctt cgctattacg ccagctggcg aaagggggat gtgctgcaag gcgattaagt
      241 tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag tgagcgcgcg
      301 taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt cctccagctt
      361 ttgttccctt tagtgagggt taattgcgcg cttggcgtaa tcatggtcat agctgtttcc
      421 tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
      481 taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gcgaagacac
      541 ctgacacccg aacaggtggt cgccattgct aataataacg gaggacggcc agccttggag
      601 tccatcgtag cccaattgtc caggcccgat cccgcgttgg ctgcgttaac gaatgaccat
      661 ctggtggcgt tggcatgtct tggtggacga cccgcgctcg atgcagtcaa aaagggtctg
      721 cctcatgctc ccgcattgat caaaagaacc aaccggcgga ttcccgagag aacttcccat
      781 cgagtcgcgg gatcccaact agtcaaaagt gaactggagg agaagaa
//
