LOCUS       sequence-107771-         826 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  pCACS backbone (Amp), LEU2, contains an RFP flanked by BsaI sites 
            with CAGT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Agmon N, Mitchell LA, Cai Y, Ikushima S, Chuang J, Zheng A, Choi WJ,
            Martin JA, Caravelli K, Stracquadanio G, Boeke JD
  TITLE     Yeast Golden Gate (yGG) for the Efficient Assembly of S. cerevisiae 
            Transcription Units.
  JOURNAL   ACS Synth Biol. 2015 Jul 17;4(7):853-9. doi: 10.1021/sb500372z. Epub
            2015 Mar 23.
  PUBMED    25756291
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb500372z";
            journalName: "ACS Synth Biol"; date: "2015-07-17- 17"; volume: "4"; 
            issue: "7"; pages: "853-9"
COMMENT     Sequence Label: pAV10.K5.loxP
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(176..209)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 cggttcagct gccgggtgct tacaaaaccg acatcaaact ggacatcacc tcccacaacg
       61 aagactacac catcgttgaa cagtacgaac gtgctgaagg tcgtcactcc accggtgctt
      121 aataacgctg atagtgctag tgtagatcgc tactagagcc aggcggtctc tactgataac
      181 ttcgtatagc atacattata cgaagttatt gctagctagt ttcatgattt tctgttacac
      241 ctaacttttt gtgtggtgcc ctcctccttg tcaatattaa tgttaaagtg caattctttt
      301 tccttatcac gttgagccat tagtatcaat ttgcttacct gtattccttt acatcctcct
      361 ttttctcctt cttgataaat gtatgtagat tgcgtatata gtttcgtcta ccctatgaac
      421 atattccatt ttgtaatttc gtgtcgtttc tattatgaat ttcatttata aagtttatgt
      481 acaaatatca taaaaaaaga gaatcttttt aagcaaggat tttcttaact tcttcggcga
      541 cagcatcacc gacttcggtg gtactgttgg aaccacctaa atcaccagtt ctgatacctg
      601 catccaaaac ctttttaact gcatcttcaa tggccttacc ttcttcaggc aagttcaatg
      661 acaatttcaa catcattgca gcagacaaga tagtggcgat agggttgacc ttattctttg
      721 gcaaatctgg agcagaaccg tggcatggtt cgtacaaacc aaatgcggtg ttcttgtctg
      781 gcaaagaggc caaggacgca gatggcaaca aacccaagga acctgg
//
