LOCUS       sequence-107791-         945 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Localization: Nucleus/Histones, Excitation: 505, Emission: 515.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  TITLE     Davidson Green fluorescent proteins
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mClover-H2B-C-10
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(143..520)
                     /codon_start=1
                     /gene="HIST1H2BJ"
                     /product="human histone H2B"
                     /label=H2B
                     /translation="PEPAKSAPAPKKGSKKAVTKAQKKGGKKRKRSRKESYSIYVYKVL
                     KQVHPDTGISSKAMGIMNSFVNDIFERIAGEASRLAHYNKRSTITSREIQTAVRLLLPG
                     ELAKHAVSEGTKAITKYTSAK"
     CDS             complement(551..802)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNSIKANFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSHQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(593..614)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gctgcataac agttacaaca acaattgcat tcattttatg tttcaggttc agggggaggt
       61 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtatggctg attatgatca
      121 gttatctaga tccggtggat ccttacttag cgctggtgta cttggtgatg gccttagtac
      181 cctcggacac ggcgtgcttg gccaactccc caggcagcag caggcgcacg gccgtctgga
      241 tctccctgga ggtgatggtc gagcgcttgt tgtaatgcgc caggcgggaa gcctcacctg
      301 cgatgcgctc gaaaatgtcg ttcacaaacg aattcatgat gcccatggcc ttggacgaaa
      361 tgccggtgtc agggtggacc tgcttcagaa ccttgtacac atagatggaa tagctctcct
      421 tgcggctgcg cttgcgcttc ttgccgcctt tcttctgcgc cttagtcacc gccttcttgg
      481 agcccttttt cggggcggga gcagacttcg ctggctctgg gcctccggag cctccagatc
      541 tgagtccgga cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
      601 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgatggc
      661 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
      721 agtggtcggc gagctgcacg ctgccgtcct caacgttgtg gcggatcttg aagttagcct
      781 tgatgctgtt cttctgcttg tcggccgtga tatagacgta gtggctgttg aagttgtact
      841 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
      901 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtct
//
