LOCUS       sequence-107811-        5863 bp    DNA     circular SYN 14-APR-2025
DEFINITION  Expresses GFP under the UbC promoter. This promoter expresses 
            transgenes in neurons at higher levels than plasmids with the CMV. 
            Optimal for tracing axons in tissue clearing procedures. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5863)
  AUTHORS   Soderblom C, Lee DH, Dawood A, Carballosa M, Jimena Santamaria A, 
            Benavides FD, Jergova S, Grumbles RM, Thomas CK, Park KK, Guest JD, 
            Lemmon VP, Lee JK, Tsoulfas P
  TITLE     3D Imaging of Axons in Transparent Spinal Cords from Rodents and 
            Nonhuman Primates
  JOURNAL   Eneuro. 2015 Mar-Apr;2(2). doi: 10.1523/ENEURO.0001-15.2015.
  PUBMED    26023683
REFERENCE   2  (bases 1 to 5863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Eneuro. 
            2015 Mar-Apr"; volume: "2(2). doi"; pages: " 
            10.1523/ENEURO.0001-15.2015"
COMMENT     Sequence Label: pAAV-UbC-eGFP
FEATURES             Location/Qualifiers
     source          1..5863
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..141
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   12..141
                     /label=AAV2 ITR
     promoter        150..549
                     /label=UbC promoter
                     /note="human ubiquitin C promoter"
     primer_bind     1119..1138
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
     primer_bind     1835..1854
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(1882..1884,1885..1887,1888..2601)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1882..1884,1885..1887,1888..2598)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1927..1948)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2188..2207)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2535..2556
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    2610..3086
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2809..2828)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   3126..3266
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   3126..3255
                     /label=AAV2 ITR
     rep_origin      3341..3796
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3428..3447)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3638..3659
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3813..3832)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3932..3954
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3992..4010)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4078..4182
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4183..4251,4252..5043)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4401..4420)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5214..5802
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5703..5722
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc
       61 gggcgacctt tggtcgcccg gcctcagtga gcgagcgagc gcgcagagag ggagtggcca
      121 actccatcac taggggttcc tgcggccgcg gcctccgcgc cgggttttgg cgcctcccgc
      181 gggcgccccc ctcctcacgg cgagcgctgc cacgtcagac gaagggcgca gcgagcgtcc
      241 tgatccttcc gcccggacgc tcaggacagc ggcccgctgc tcataagact cggccttaga
      301 accccagtat cagcagaagg acattttagg acgggacttg ggtgactcta gggcactggt
      361 tttctttcca gagagcggaa caggcgagga aaagtagtcc cttctcggcg attctgcgga
      421 gggatctccg tggggcggtg aacgccgatg attatataag gacgcgccgg gtgtggcaca
      481 gctagttccg tcgcagccgg gatttgggtc gcggttcttg tttgtggatc gctgtgatcg
      541 tcacttggtg agtagcgggc tgctgggctg gccggggctt tcgtggccgc cgggccgctc
      601 ggtgggacgg aagcgtgtgg agagaccgcc aagggctgta gtctgggtcc gcgagcaagg
      661 ttgccctgaa ctgggggttg gggggagcgc agcaaaatgg cggctgttcc cgagtcttga
      721 atagaacctt cgctaatgcg ggaaagctct tattcgggtg agatgggctg gggcaccatc
      781 tggggaccct gacgtgaagt ttgtcactga ctggagaact cggtttgtcg tctgttgcgg
      841 gggcggcagt tatggcggtg ccgttgggca gtgcacccgt acctttggga gcgcgcgccc
      901 tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg gccacctgcc
      961 ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc tagggtaggc
     1021 tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga ggcgtcagtt
     1081 tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt tttgaactat
     1141 gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg aaatgtaatc
     1201 atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta aattctggcc
     1261 gtttttggct tttttgttag acgaagctaa ggcgcgcctg agaacttcag ggtgagtttg
     1321 gggacccttg attgttcttt ctttttcgct attgtaaaat tcatgttata tggagggggc
     1381 aaagttttca gggtgttgtt tagaatggga agatgtccct tgtatcacca tgcatggacc
     1441 ctcatgataa ttttgtttct ttcactttct actctgttga caaccattgt ctcctcttat
     1501 tttcttttca ttttctgtaa ctttttcgtt aaactttagc ttgcatttgt aacgaatcta
     1561 gacaatcagg gtatattata ttgtacttca gcacagtttt agagaacaat tgttataatt
     1621 aaatgataag gtagaatatt tctgcatata aattctggct ggcgtggaaa tattcttatt
     1681 ggtagaaaca actacatcct ggtcatcatc ctgcctttct ctttatggtt acaatgatat
     1741 acactgtttg agatgaggat aaaatactct gagtccaaac cgggcccctc tgctaaccat
     1801 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     1861 catcaagctt aaggatccac catggtgagc aagggcgagg agctgttcac cggggtggtg
     1921 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
     1981 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
     2041 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
     2101 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
     2161 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
     2221 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
     2281 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
     2341 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
     2401 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
     2461 gtgctgctgc ccgacaacca ctacctgagc acccagtccg ctctgagcaa agaccccaac
     2521 gagaagcgcg atcacatggt cctgctggag ttcgtgaccg ccgccgggat cactctcggc
     2581 atggacgagc tgtacaagtg aagatctacg ggtggcatcc ctgtgacccc tccccagtgc
     2641 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa
     2701 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg
     2761 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac
     2821 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag
     2881 cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca
     2941 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca
     3001 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt
     3061 gaaccactgc tcccttccct gtccttctga ttttgtaggt aaccacgtgc ggaccgagcg
     3121 gccgcaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac
     3181 tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag
     3241 cgagcgagcg cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct
     3301 gtgcggtatt tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca
     3361 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     3421 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     3481 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     3541 cccaaaaaac ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     3601 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     3661 acaacactca accctatctc gggctattct tttgatttat aagggatttt gccgatttcg
     3721 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     3781 ttaacgttta caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta
     3841 agccagcccc gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg
     3901 gcatccgctt acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca
     3961 ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt
     4021 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
     4081 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
     4141 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
     4201 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
     4261 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
     4321 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
     4381 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
     4441 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
     4501 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
     4561 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
     4621 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
     4681 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
     4741 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
     4801 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
     4861 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
     4921 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
     4981 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
     5041 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
     5101 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
     5161 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     5221 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     5281 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     5341 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     5401 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     5461 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     5521 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     5581 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
     5641 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     5701 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     5761 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     5821 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgt
//
