LOCUS       sequence-107929-         879 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  A reporter plasmid containing the attB and attP sites flanking a 
            green fluorescent protein reporter gene (gfp)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 879)
  AUTHORS   Yang L, Nielsen AA, Fernandez-Rodriguez J, McClune CJ, Laub MT, Lu 
            TK, Voigt CA
  TITLE     Permanent genetic memory with >1-byte capacity.
  JOURNAL   Nat Methods. 2014 Dec;11(12):1261-6. doi: 10.1038/nmeth.3147. Epub 
            2014 Oct 26.
  PUBMED    25344638
REFERENCE   2  (bases 1 to 879)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nmeth.3147"; journalName: "Nat Methods"; date: "2014-12"; 
            volume: "11"; issue: "12"; pages: "1261-6"
COMMENT     Sequence Label: pReporter_9
FEATURES             Location/Qualifiers
     source          1..879
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..23
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     misc_RNA        complement(54..103)
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     terminator      complement(174..201)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(217..288)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(511..528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(725..744)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctcatctagt atttctcctc tttctctaga ttaaacaaaa ttatttgtag aggctgtttc
       61 gtcctcacgg actcatcaga ccggaaagca catccggtga cagcttaatt ggaagttcgg
      121 aataactatg cagatacctg atacacactt ccaacaaaaa caaccactat aaacgcagaa
      181 aggcccaccc gaaggtgagc cagtgtgact ctagtagaga gcgttcaccg acaaacaaca
      241 gataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat gcctggctag
      301 ggcccatacc caaaaaaaaa ccccgcccct gacagggcgg ggtttttttt aggtagagac
      361 ccacgaggca gacctcagcg ctagcggagt gtatactggc ttactatgtt ggcactgatg
      421 agggtgtcag tgaagtgctt catgtggcag gagaaaaaag gctgcaccgg tgcgtcagca
      481 gaatatgtga tacaggatat attccgcttc ctcgctcact gactcgctac gctcggtcgt
      541 tcgactgcgg cgagcggaaa tggcttacga acggggcgga gatttcctgg aagatgccag
      601 gaagatactt aacagggaag tgagagggcc gcggcaaagc cgtttttcca taggctccgc
      661 ccccctgaca agcatcacga aatctgacgc tcaaatcagt ggtggcgaaa cccgacagga
      721 ctataaagat accaggcgtt tccccctggc ggctccctcg tgcgctctcc tgttcctgcc
      781 tttcggttta ccggtgtcat tccgctgtta tggccgcgtt tgtctcattc cacgcctgac
      841 actcagttcc gggtaggcag ttcgctccaa gctggactg
//
