LOCUS       sequence-107940-        1458 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expresses LAR-GECO1.2 in the mitochondria in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1458)
  AUTHORS   Wu J, Prole DL, Shen Y, Lin Z, Gnanasekaran A, Liu Y, Chen L, Zhou 
            H, Chen SR, Usachev YM, Taylor CW, Campbell RE
  TITLE     Red fluorescent genetically encoded Ca2+ indicators for use in 
            mitochondria and endoplasmic reticulum.
  JOURNAL   Biochem J. 2014 Nov 15;464(1):13-22. doi: 10.1042/BJ20140931.
  PUBMED    25164254
REFERENCE   2  (bases 1 to 1458)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Biochem J"; date: "2014-11-15- 15"; volume: "464"; issue: "1"; 
            pages: "13-22"
COMMENT     Sequence Label: CMV-mito-LAR-GECO1.2
FEATURES             Location/Qualifiers
     source          1..1458
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..75
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     CDS             97..171
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
     primer_bind     972..991
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atgtctgttc tgactcctct gctgctccgg ggtctcacag gttccgcaag aagactcccc
       61 gtgcctaggg ccaaaattca ttcactgggg gaccccatga gcgtgctcac cccactcctg
      121 ctgcgggggc tgaccggcag cgctaggcgg ctgccagtcc ccagggccaa gatccacagt
      181 ctcggcgatc ccaaggatcc aaccatggtc gactcttcac gtcgtaagtg gattaagcgt
      241 ggtcacgcag tcagagctat aggtcggctg agctcacccg tggtttccga gcggatgtac
      301 cccgaggacg gcgctctgaa gagcgagatc aagaaggggc tgaggctgaa ggacggcggc
      361 cactacgccg ccgaggtcaa gaccacctac aaggccaaga agcccgtgca gctgcccggc
      421 gcctacatag tcgacatcaa gttggacatc gtgtcccaca acgaggacta caccatcgtg
      481 gaacagtgcg aacgcgccgt gggccgccac tccaccggcg gcatggacga gctgtacaag
      541 ggaggtacag gcgggagtct ggtgagcaag ggcgaggagg ataacagggc catcgtcaag
      601 gagttcatgc gcttcaagtt gcacatggag ggctccgtga acggccacga gttcgagatc
      661 gagggcgagg gcgagggccg cccctacgag gcctttcaga ccgctaagct gaaggtgacc
      721 aagggtggcc ccctgccctt cgcatgggac atcctgtccc ctcagttgat gtacggctcc
      781 aaggcctaca ttaagcaccc agccgacatc cccgactact tcaagctgtc cttccccgag
      841 ggcttcaggt gggagcgcgt gatgaacttc gaggacggcg gcattattca cgttagccag
      901 gacacctccc tgcaggacgg cgtattcatc tacaaggtga agctgcgcgg caccaacttc
      961 ccccccgacg gccccgtaat gcagaagaag accatgggct gggaggctac gcgtgaccaa
     1021 ctgactgaag agcagatcgc agaatttaaa gaggctttct ccctatttga cgaggacggg
     1081 gatgggacga tgacaaccaa ggagctgggg acggtgatgc ggtctctggg gcagaacccc
     1141 acagaagcag agctgcagga catgatcaat gaagtagatg ccgacggtga cggcacattc
     1201 gacttccctg agttcctgac gatgatggca agaaaaatga atgacacaga cagtgaagag
     1261 gaaattagag aagcgttccg cgtgtttgat aaggacggca atggctacat cggcgcagca
     1321 gagcttcgcc acgtgatgac agaccttgga gagaagataa cagatgagga ggttgatgaa
     1381 atgatcaggg tagcagacat cgatggggat ggtcaggtaa actacgaaga gtttgtacaa
     1441 atgatgacag cgaagtaa
//
