LOCUS       sequence-108118-         778 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  E. coli expression plasmid, useful in conjunction with pUCXKT for 
            positive selection cloning and expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Prosser GA, Williams EM, Sissons JA, Walmsley KE, Parker MR, 
            Ackerley DF
  TITLE     A gain-of-function positive-selection expression plasmid that 
            enables high-efficiency cloning.
  JOURNAL   Biotechnol Lett. 2014 Sep 26.
  PUBMED    25257589
REFERENCE   2  (bases 1 to 778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biotechnol 
            Lett. 2014 Sep 26."
COMMENT     Sequence Label: pUCX
FEATURES             Location/Qualifiers
     source          1..778
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(2..79)
                     /gene="lacI (mutant)"
                     /label=lacIq promoter
                     /note="In the lacIq allele, a single base change in the 
                     promoter boosts expression of the lacI gene about 10-fold."
     promoter        join(309..314,315..330,331..337)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    341..365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             380..402
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(480..497)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      650..736
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 cattcaccac cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt
       61 tgcaccattc gatggtgtca acgtaaatgc cgcttcgcct tcgcgcgcga attgcaagct
      121 gatccgggct tatcgactgc acggtgcacc aatgcttctg gcgtcaggca gccatcggaa
      181 gctgtggtat ggctgtgcag gtcgtaaatc actgcataat tcgtgtcgct caaggcgcac
      241 tcccgttctg gataatgttt tttgcgccga catcataacg gttctggcaa atattctgaa
      301 atgagctgtt gacaattaat catcggctcg tataatgtgt ggaattgtga gcggataaca
      361 attcccctct agaaataatt ttgtttaact ttaagaagga gatatcatat gggatcctct
      421 agagtcgacc tgcaggcatg caagcttggc tgttttggcg gatgagagaa gattttcagc
      481 ctgatacaga ttaaatcaga acgcagaagc ggtctgataa aacagaattt gcctggcggc
      541 agtagcgcgg tggtcccacc tgaccccatg ccgaactcag aagtgaaacg ccgtagcgcc
      601 gatggtagtg tggggtctcc ccatgcgaga gtagggaact gccaggcatc aaataaaacg
      661 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
      721 cctgagtagg acaaatccgc cgggagcgga tttgaacgtt gcgaagcaac ggcccgga
//
