LOCUS       sequence-108354-         972 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Cloning and expression vector for Clostridium thermocellum.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Olson DG, Lynd LR
  TITLE     Computational design and characterization of a temperature-sensitive
            plasmid replicon for gram positive thermophiles.
  JOURNAL   J Biol Eng. 2012 May 11;6(1):5. doi: 10.1186/1754-1611-6-5.
  PUBMED    22578246
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Eng."; date: "2012-05-11"; volume: "6(1):5. doi"; pages: " 
            10.1186/1754-1611-6-5"
COMMENT     Sequence Label: pMU102
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(292..308)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(292..308)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(305..327)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    316..332
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(340..346,347..364,365..370))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    385..406
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
ORIGIN
        1 ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac
       61 tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta
      121 aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt
      181 taccaatgct taatcagtga ggcacctatc tcagcgatct gtcaattcga gctcggtacc
      241 cggggatcct ctagagtcga cctgcaggca tgcaagcttg gcgtaatcat ggtcatagct
      301 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
      361 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
      421 actgcccgct ttccagtcgg gaaacctgtc gtgccagccc ttcaaacttc ccaaaggcga
      481 gccctagtga cattagaaaa ccgactgtaa aaagtacagt cggcattatc tcatattata
      541 aaagccagtc attaggccta tctgacaatt cctgaataga gttcataaac aatcctgcat
      601 gataaccatc acaaacagaa tgatgtacct gtaaagatag cggtaaatat attgaattac
      661 ctttattaat gaattttcct gctgtaataa tgggtagaag gtaattacta ttattattga
      721 tatttaagtt aaacccagta aatgaagtcc atggaataat agaaagagaa aaagcatttt
      781 caggtatagg tgttttggga aacaatttcc ccgaaccatt atatttctct acatcagaaa
      841 ggtataaatc ataaaactct ttgaagtcat tctttacagg agtccaaata ccagagaatg
      901 ttttagatac accatcaaaa attgtataaa gtggctctaa cttatcccaa taacctaact
      961 ctccgtcgct at
//
