LOCUS       sequence-108391-         942 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  ST1-dCas9 with VP64-p65-Rta (VPR) fused to it's C-terminus; 
            mammalian vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 942)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: M_ST1n_VPR
FEATURES             Location/Qualifiers
     source          1..942
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..28
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
       61 ccatagaaga caccgggacc gatccagcct ccggactcta gaggatcgaa cccttgccac
      121 catgagcgac ctggtgctgg gcctggccat cggcatcggc agcgtgggcg tgggcatcct
      181 gaacaaggtg accggcgaga tcatccacaa gaacagtcgc atcttccctg ctgctcaggc
      241 tgagaacaac ctggtgcgcc gcaccaaccg ccagggtcgc cggcttgctc gccgcaagaa
      301 gcaccggcgc gtgcgcctga accgcctgtt cgaggagagc ggcctgatca ccgacttcac
      361 caagatcagc atcaacctga acccctacca gctgcgcgtg aagggcctga ccgacgagct
      421 gagcaacgag gagctgttca tcgccctgaa gaacatggtg aagcaccgcg gcatcagcta
      481 cctggacgac gccagcgacg acggcaacag cagcgtgggc gactacgccc agatcgtgaa
      541 ggagaacagc aagcagctgg agaccaagac ccccggccag atccagctgg agcgctacca
      601 gacctacggc cagctgcgcg gcgacttcac cgtggagaag gacggcaaga agcaccgcct
      661 gatcaacgtg ttccccacca gcgcctaccg cagcgaggcc ctgcgcatcc tgcagaccca
      721 gcaggagttc aacccccaga tcaccgacga gttcatcaac cgctacctgg agatcctgac
      781 cggcaagcgc aagtactacc acggccccgg caacgagaag agccgcaccg actacggccg
      841 ctaccgcacc agcggcgaga ccctggacaa catcttcggc atcctgatcg gcaagtgcac
      901 cttctacccc gacgagttcc gcgccgccaa ggccagctac ac
//
