LOCUS       sequence-108398-         741 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  pAG414 series plasmid with GPD promoter driving expression of 
            dCas9-VPR; cerevisiae vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 741)
  AUTHORS   Chavez A, Scheiman J, Vora S, Pruitt BW, Tuttle M, P R Iyer E, Lin 
            S, Kiani S, Guzman CD, Wiegand DJ, Ter-Ovanesyan D, Braff JL, 
            Davidsohn N, Housden BE, Perrimon N, Weiss R, Aach J, Collins JJ, 
            Church GM
  TITLE     Highly efficient Cas9-mediated transcriptional programming.
  JOURNAL   Nat Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312.
  PUBMED    25730490
REFERENCE   2  (bases 1 to 741)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Mar 2. doi: 10.1038/nmeth.3312."
COMMENT     Sequence Label: pAG414GPD-dCas9-VPR
FEATURES             Location/Qualifiers
     source          1..741
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    39..63
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(68..637)
                     /codon_start=1
                     /product="transcriptional activation domain from the human 
                     herpesvirus 4 (Epstein-Barr virus) replication and 
                     transcription activator Rta/BRLF1 (Hardwick et al., 1992; 
                     Chavez et al., 2015)"
                     /label=Rta AD
                     /translation="RDSREGMFLPKPEAGSAISDVFEGREVCQPKRIRPFHPPGSPWAN
                     RPLPASLAPTPTGPVHEPVGSLTPAPVPQPLDPAPAVTPEASHLLEDPDEETSQAVKAL
                     REMADTVIPQKEEAAICGQMDLSHPPPRGHLDELTTTLESMTEDLNLDSPLTPELNEIL
                     DTFLNDECLLHAMHISTGLSIFDTSLF"
ORIGIN
        1 gtatcgataa gcttgatatc gaattcctgc agcccatcac cactttgtac aagaaagctg
       61 ggtttcaaaa cagagatgtg tcgaagatgg acagtcctgt gctgatatgc atggcatgca
      121 agaggcactc gtcgttcagg aaggtatcca gaatctcgtt caattccggg gtcaggggtg
      181 agtccaggtt cagatcctcg gtcatggact caagtgtggt tgtcagctca tccagatggc
      241 cccttggggg cggatgggaa aggtccattt ggccacagat tgcagcctct tccttctggg
      301 gaatcacagt atcggccatc tcccgaaggg ctttgacagc ctggctcgtc tcttcatcgg
      361 gatcctccaa caggtgactg gcctcgggag tcactgcggg cgctggatcc agtggctgag
      421 ggactggtgc cggggtcagt gacccgactg gctcatgtac tggaccggtt ggtgttggtg
      481 cgaggctggc ggggagtggg cggttggccc atggacttcc tggaggatga aatggccgga
      541 ttcgttttgg ctggcacacc tcgcggccct caaacacgtc actaatagcg gagccggcct
      601 caggcttcgg caaaaacatc ccttccctgg aatcccgaga tcccgatccg ctacccagca
      661 gtgcggagaa gtccatgtcg gcgatagagc tgaagtcctc gtcgccagac agcagtccat
      721 taggcaggcc tggtgctccc a
//
