LOCUS       sequence-108568-         833 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Gateway compatible Y2H vector - BD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 833)
  AUTHORS   Lu Q, Tang X, Tian G, Wang F, Liu K, Nguyen V, Kohalmi SE, Keller 
            WA, Tsang EW, Harada JJ, Rothstein SJ, Cui Y
  TITLE     Arabidopsis homolog of the yeast TREX-2 mRNA export complex: 
            components and anchoring nucleoporin.
  JOURNAL   Plant J. 2010 Jan;61(2):259-70. doi: 
            10.1111/j.1365-313X.2009.04048.x. Epub 2009 Oct 16.
  PUBMED    19843313
REFERENCE   2  (bases 1 to 833)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1111/j.1365-313X.2009.04048.x"; journalName: "Plant J"; date: 
            "2010-01"; volume: "61"; issue: "2"; pages: "259-70"
COMMENT     Sequence Label: pGBKT7-GW
FEATURES             Location/Qualifiers
     source          1..833
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(108..232)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      256..303
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     terminator      330..517
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(385..409)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(541..557)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(541..557)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(554..576)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    565..581
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(589..595,596..613,614..619))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    634..655
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(772..789)
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga tgttctgggg
       61 aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat agtgactgga
      121 tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa tttaatatat
      181 tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg gtctcgacct
      241 gcagcggccg cataactagc ataacccctt ggggcctcta aacgggtctt gaggggtttt
      301 ttgcgcgctt gcagccaagc taattccggg cgaatttctt atgatttatg atttttatta
      361 ttaaataagt tataaaaaaa ataagtgtat acaaatttta aagtgactct taggttttaa
      421 aacgaaaatt cttattcttg agtaactctt tcctgtaggt caggttgctt tctcaggtat
      481 agcatgaggt cgctcttatt gaccacacct ctaccggcat gcaagcttgg cgtaatcatg
      541 gtcatagctg tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc
      601 cggaagcata aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc
      661 gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat
      721 cggccaacgc gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac
      781 tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caa
//
