LOCUS       sequence-108665-         938 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Chr.13/CLYBL TALEN expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
  TITLE     Transcription activator-like effector nuclease (TALEN)-mediated 
            CLYBL targeting enables enhanced transgene expression and one-step 
            generation of dual reporter human induced pluripotent stem cell 
            (iPSC) and neural stem cell (NSC) lines.
  JOURNAL   PLoS One. 2015 Jan 14;10(1):e0116032. doi: 
            10.1371/journal.pone.0116032. eCollection 2015.
  PUBMED    25587899
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2015-01-14"; volume: "10(1):e0116032. doi"; pages: " 
            10.1371/journal.pone.0116032. eCollection 2015"
COMMENT     Sequence Label: pZT-C13-L1
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(31..618)
                     /codon_start=1
                     /product="nonspecific DNA cleavage domain of the FokI 
                     endonuclease (Li et al., 1992)"
                     /label=FokI cleavage domain
                     /note="must dimerize to be catalytically active"
                     /translation="QLVKSELEEKKSELRHKLKYVPHEYIELIEIARNSTQDRILEMKV
                     MEFFMKVYGYRGKHLGGSRKPDGAIYTVGSPIDYGVIVDTKAYSGGYNLPIGQADEMQR
                     YVEENQTRNKHINPNEWWKVYPSSVTEFKFLFVSGHFKGNYKAQLTRLNHITNCNGAVL
                     SVEELLIGGEMIKAGTLTLEEVRRKFNNGEINF"
ORIGIN
        1 gggccctcta gactcgagtt atcaagatct aaagtttatc tcgccgttat taaatttccg
       61 tctgacttcc tctaaggtta atgtgccggc tttaatcatt tctccaccaa ttaaaagctc
      121 ttctacacta agaacagctc cattacaatt agtgatatga tttaatcgtg taagctgagc
      181 tttgtagttt cctttaaagt gaccactcac aaataaaaac ttaaattccg ttacagaaga
      241 tggatagact ttccaccatt cattagggtt gatatgtttg tttcgtgttt gattttcttc
      301 gacatatcgt tgcatttcat ctgcttggcc aattggcaga ttataacctc cgctataagc
      361 tttagtatcc acgatcacac cgtaatcaat aggagatccg acagtataaa ttgctccgtc
      421 cggtttcctt gatccaccca aatgtttacc tctatatcca taaactttca taaaaaattc
      481 cattaccttc atttcaagaa ttctatcctg agtggaattt ctggcaattt caattaattc
      541 aatatattca tgaggcacat atttcaattt atgacgaagt tcagatttct tctcctccag
      601 ttcacttttg actagttggg atcccgcgac tcgatgggaa gttctctcgg gaatccgccg
      661 gttggttctt ttgatcaatg cgggagcatg aggcagaccc tttttgactg catcgagcgc
      721 gggacgtcca ccaagacatg ccaacgccac cagatggtcg ttggtcaacg cggccaacgc
      781 cggatcaggc cggctcagct gggccacaat gctttcgagc gcttgcttgc cgccaccgtt
      841 gctggcgata gccaccactt ggtccggggt caggccatgg tcctggcaca gcaccggcaa
      901 cagccgctgc accgtttcga gcgcttgctt gccgccac
//
