LOCUS       sequence-108757-         717 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  MuLE (Multiple Lentiviral Expression) Entry vector containing CMV 
            promoter and human codon optimized Cas9 module Compatible with 
            MultiSite Gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 717)
  AUTHORS   Albers J, Danzer C, Rechsteiner M, Lehmann H, Brandt LP, Hejhal T, 
            Catalano A, Busenhart P, Goncalves AF, Brandt S, Bode PK, 
            Bode-Lesniewska B, Wild PJ, Frew IJ
  TITLE     A versatile modular vector system for rapid combinatorial mammalian 
            genetics.
  JOURNAL   J Clin Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743.
  PUBMED    25751063
REFERENCE   2  (bases 1 to 717)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Clin 
            Invest. 2015 Mar 9. pii: 79743. doi: 10.1172/JCI79743."
COMMENT     Sequence Label: pMuLE ENTR CMV-hCas9 R4-R3
FEATURES             Location/Qualifiers
     source          1..717
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     25..44
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        25..43
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      63..72
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc aagcttggta
       61 ccgccaccat ggacaagaag tactccattg ggctcgatat cggcacaaac agcgtcggct
      121 gggccgtcat tacggacgag tacaaggtgc cgagcaaaaa attcaaagtt ctgggcaata
      181 ccgatcgcca cagcataaag aagaacctca ttggcgccct cctgttcgac tccggggaga
      241 cggccgaagc cacgcggctc aaaagaacag cacggcgcag atatacccgc agaaagaatc
      301 ggatctgcta cctgcaggag atctttagta atgagatggc taaggtggat gactctttct
      361 tccataggct ggaggagtcc tttttggtgg aggaggataa aaagcacgag cgccacccaa
      421 tctttggcaa tatcgtggac gaggtggcgt accatgaaaa gtacccaacc atatatcatc
      481 tgaggaagaa gcttgtagac agtactgata aggctgactt gcggttgatc tatctcgcgc
      541 tggcgcatat gatcaaattt cggggacact tcctcatcga gggggacctg aacccagaca
      601 acagcgatgt cgacaaactc tttatccaac tggttcagac ttacaatcag cttttcgaag
      661 agaacccgat caacgcatcc ggagttgacg ccaaagcaat cctgagcgct aggctgt
//
