LOCUS       sequence-109044-       10569 bp    DNA     circular SYN 14-APR-2025
DEFINITION  KIF1B beta aa 387-1770 with N-terminal FRB and triple myc tags.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Bentley M, Decker H, Luisi J, Banker G
  TITLE     A novel assay reveals preferential binding between Rabs, kinesins, 
            and specific endosomal subpopulations.
  JOURNAL   J Cell Biol. 2015 Feb 2;208(3):273-281. Epub 2015 Jan 26.
  PUBMED    25624392
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2015-02-2"; volume: "208(3)"; pages: "273-281. Epub 
            2015 Jan 26"
COMMENT     Sequence Label: pBa-FRB-3myc-KIF1B beta tail 387-1770
FEATURES             Location/Qualifiers
     source          1..10569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        418..693
                     /label=chicken beta-actin promoter
     primer_bind     1626..1648
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     CDS             1728..2006
                     /codon_start=1
                     /product="T2098L mutant of FKBP-rapamycin binding domain of
                     human FRAP"
                     /label=FRB*
                     /note="binds synthetic ligands such as AP21967"
                     /translation="ILWHEMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTL
                     KETSFNQAYGRDLMEAQEWCRKYMKSGNVKDLLQAWDLYYHVFRRISK"
     polyA_signal    6406..6540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6443..6462)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6497..6516
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6572..6588)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6572..6588)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6585..6607)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6596..6612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6620..6626,6627..6644,6645..6650))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6665..6686
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6803..6820)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6974..7562)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7054..7073)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7733..8524,8525..8593))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8356..8375
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8594..8698)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8766..8784
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8822..8844)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8944..8963
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     9157..9179
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9171..9188
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9172..9188
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttagtgcatg catacccctc ggtgttattg ctgctctgtg cacgcacgct cattgtatca
       61 cttcatccca gtgcatgcac tcacactgga gcgattgctg ctcggtgcac gcacactcat
      121 tgtatcacgt cagctcagtg gctgcacgca caccggtgtt attgctgctc ggtgcgtgca
      181 tgcacatcag tgtcgctgca gctcagtgca tgcacgctca ttgcccatcg ctatccctgc
      241 ctctcctgct ggcgctcccc gggaggtgac ttcaagggga ccgcaggacc acctcggggg
      301 tggggggagg gctgcacacg cggaccccgc tccccctccc caacaaagca ctgtggaatc
      361 aaaaaggggg gaggggggat ggaggggcgc gtcacacccc cgccccacac cctcacctcg
      421 aggtgagccc cacgttctgc ttcactctcc ccatctcccc cccctcccca cccccaattt
      481 tgtatttatt tattttttaa ttattttgtg cagcgatggg ggcggggggg gggggggcgc
      541 gcgccaggcg gggcggggcg gggcgagggg cggggcgggg cgaggcggag aggtgcggcg
      601 gcagccaatc agagcggcgc gctccgaaag tttcctttta tggcgaggcg gcggcggcgg
      661 cggccctata aaaagcgaag cgcgcggcgg gcgggagtcg ctgcgttgcc ttcgccccgt
      721 gccccgctcc gcgccgcctc gcgccgcccg ccccggctct gactgaccgc gttactccca
      781 caggtgagcg ggcgggacgg cccttctcct ccgggctgta attagcgctt ggtttaatga
      841 cggctcgttt cttttctgtg gctgcgtgaa agccttaaag ggctccggga gggccctttg
      901 tgcggggggg agcggctcgg ggggtgcgtg cgtgtgtgtg tgcgtgggga gcgccgcgtg
      961 cggcccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg gcgcggggct ttgtgcgctc
     1021 cgcgtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg ggggctgcga
     1081 ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg tgagcagggg gtgtgggcgc
     1141 ggcggtcggg ctgtaacccc cccctgcacc cccctccccg agttgctgag cacggcccgg
     1201 cttcgggtgc ggggctccgt gcggggcgtg gcgcggggct cgccgtgccg ggcggggggt
     1261 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
     1321 aggggcgcgg cggccccgga gcgccggcgg ctgtcgaggc gcggcgagcc gcagccattg
     1381 ccttttatgg taatcgtgcg agagggcgca gggacttcct ttgtcccaaa tctggcggag
     1441 ccgaaatctg ggaggcgccg ccgcaccccc tctagcgggc gcgggcgaag cggtgcggcg
     1501 ccggcaggaa ggaaatgggc ggggagggcc ttcgtgcgtc gccgcgccgc cgtccccttc
     1561 tccatctcca gcctcggggc tgccgcaggg ggacggctgc cttcgggggg gacggggcag
     1621 ggcggggttc ggcttctggc gtgtgaccgg cggggtttat atcttccctt ctctgttcct
     1681 ccgcagcccc caagcttggc gcgcctaggc ctaccatggc ttctagaatc ctctggcatg
     1741 agatgtggca tgaaggcctg gaagaggcat ctcgtttgta ctttggggaa aggaacgtga
     1801 aaggcatgtt tgaggtgctg gagcccttgc atgctatgat ggaacggggc ccccagactc
     1861 tgaaggaaac atcctttaat caggcctatg gtcgagattt aatggaggcc caagagtggt
     1921 gcaggaagta catgaaatca gggaatgtca aggacctcct ccaagcctgg gacctctatt
     1981 atcatgtgtt ccgacgaatc tcaaagacta gttatccgta cggaatgcag aagctgatct
     2041 cagaggagga cctgcaaaaa cttatttctg aagaggatct gcaaaagctt atttctgaag
     2101 aagatctgta caagggagga tccggaggcg atacatccat ggggtccctc acttcatccc
     2161 cgtcttcatg ctcacttaat agtcaggcgg gcctaacctc tgtgaccagt attcaagaga
     2221 ggattatgtc tacacctgga ggggaggaag ccattgaacg tctgaaggag tcagagaaga
     2281 tcattgctga gttgaatgaa acctgggaag agaaacttcg taaaacagag gccatcagaa
     2341 tggagagaga agccttgttg gctgagatgg gagttgccat acgggaagat gggggaacac
     2401 ttggcgtttt ctcacctaaa aagactccac accttgttaa cctcaatgaa gaccctctaa
     2461 tgtcggagtg tttgctttat tatatcaagg acggaattac aagggttggc caagcagatg
     2521 ctgagcggcg ccaggacatc gtgctgagtg gggcccacat taaagaagag cattgtatct
     2581 ttcggagtga gagaaacaac actggcgaag ttattgtgac cttagagccc tgtgagcgct
     2641 cagaaacata cgtgaatggc aagagggtgg ctcatcctgt tcagctgcgc tcagggaacc
     2701 gtatcatcat gggtaaaaac catgtttttc gttttaacca ccctgaacaa gcgcgagcag
     2761 aaagggaaaa gactccctca gctgagaccc cctctgagcc tgtggactgg acctttgctc
     2821 agagagagct tctcgaaaaa caaggaattg atatgaaaca ggagatggaa aagaggcttc
     2881 aggaaatgga gatcctttac aaaaaggaga aggaggaagc cgatcttctc ctggagcagc
     2941 agagactgga ctacgagagt aaattgcagg ccttgcagag gcaggttgag actcgatctc
     3001 tggctgcaga gacaacggaa gaggaggaag aggaggagga agttccttgg acacagcatg
     3061 aatttgagtt ggcccagtgg gcgttccgga aatggaagtc tcaccagttt acttcattaa
     3121 gggacttact ctggggcaat gcggtgtacc tgaaggaagc caatgccatc agtgtggaac
     3181 taaagaaaaa ggtgcagttc cagtttgttc tgctgactga cacgctgtac tcccctgtgc
     3241 ctcctgaact gcttccctcg gagatggaga agacgcacga ggacagacct ttccctcgga
     3301 ctgtggtggc ggtggaggtc caggatctga agaatggagc aacacattat tggtctttgg
     3361 ataaactcaa gcagagactg gatctgatgc gagagatgta tgacagggca ggggaggtgg
     3421 cctccagtgc ccaagatgac agcgagacga ccatgaccgg cagcgaccca ttctacgatc
     3481 ggttccactg gttcaaactt gtgggaagct cccctatttt ccacggctgt gtgaatgagc
     3541 gccttgccga ccgcacaccc tcccccactt tttccacggc cgattccgac atcactgaac
     3601 tggctgacga gcagcaggat gccatggagg actttgatga tgaggcattc gtggatgaca
     3661 ccggctctga cgcagggacg gaagagggat cagagctctt cagtgatggg catgacccat
     3721 tttacgaccg atccccttgg ttcattttag taggaagggc atttgtgtac ttgagcaacc
     3781 tgctgtatcc ggtgcccctg attcacaggg tggccattgt cagtgagaag ggggaagtcc
     3841 ggggatttct tcgtgtggct gtgcaggcca ttgcagcgga tgaagaagcc cctgattatg
     3901 gctctggaat ccggcagtcg ggaacagcta aaatatcttt tgataatgaa tactttaatc
     3961 agagtgactt ctcgtcagct gcaatgactc gttctggtct gtccttggag gagctgagga
     4021 ttgtggaagg tcagggccag agttctgaag tcatcagtcc tccagaagaa gtcaatcgaa
     4081 tgaacgacct ggatttgaag tcaggcactt tgctggatgg gaagatggtg atggaagggt
     4141 tttctgaaga gattggtaac cacctgaagc tgggcagtgc cttcactttc cgagtcactg
     4201 tgctccaagc cagcgggatc cttccagagt atgcagacat cttctgtcag ttcaactttt
     4261 tgcatcggca tgatgaagcg ttctccactg aacccctcaa gaacaatgga agaggaagtc
     4321 ccctgggctt ctaccatgta cagaacattg cggtggaggt cacagagtcg tttgtggact
     4381 acatcaaaac caagcccatt gtattcgagg tctttgggca ctaccagcag cacccactcc
     4441 atctgcaagg acaagatctt aacagtccac ctcagccatc tcggagattc ttccccccac
     4501 ccatgccact ctccaagcca gttccagcta ctaagttaaa caccatgaac aaaaccaccc
     4561 ttgggcaaag catgagcaag tacgaccttc tggtttggtt tgagatcagt gaactggagc
     4621 ctactggaga gtatattcca gctgtggttg accatactgc aggcctgccc tgccagggga
     4681 catttttgct gcaccagggc atccagcgac ggatcacagt gactatcatc cacgagaagg
     4741 gcagtgagct ccactggaaa gatgtccgtg agctggtggt aggtcggatt aggaataaac
     4801 cggaggtaga tgaagctgca gttgatgctg ttctttccct gaacatcatc tctgctaagt
     4861 ccctgaaggc tgcccacagc tccagcagga ccttctaccg tttcgaggca gtatgggaca
     4921 gctccctaca taactccctc cttctgaacc gagtgacacc ctatggagaa aagatctaca
     4981 tgaccttatc tgcttatctg gagttggatc attgcatcca gccggccgtc atcactaagg
     5041 atgtgtgcat ggtcttctat tcccgagatg ccaagatctc accaccgcgt tctctgcgaa
     5101 acctctttgg tagtggctac tcaaagtcac cagactccaa ccgagtgact gggatctatg
     5161 agctgagctt atgcaaaatg gcagatactg gaagcccagg catgcagagg cggaggagaa
     5221 aagtcttgga tacctcagtg gcatatgtgc ggggagagga gaacctagcg ggctggcgac
     5281 ctcgtggaga cagcctcatt ctggaacacc agtgggagct ggagaagctg gagctgctgc
     5341 atgaggtgga aaaaactcgc cacttcttgc tgcttcggga gagacttggt gacagcgtcc
     5401 ctaagtctct gagtgactca ctgtccccca gcctgagcag tggaaccctt agcacctcca
     5461 ccagcatctc ctctcagatc tcaaccacca cctttgaaag cgccatcaca ccgagcgaga
     5521 gcagtggcta cgactcggca gacgttgaaa gcctggtcga ccgggagaaa gagctggcta
     5581 caaagtgcct gcagcttctc acccacactt tcaaccgaga attcagccag gtgcacggca
     5641 gcatcagtga ctgtaagttg tctgacatct ctccaattgg acgggacccc tctgtgtcca
     5701 gtttcagcag ttccaccctc acgccctcct ccacttgccc ctccttggtg gactccagga
     5761 gcagctctat ggatcagaag accccagaag ccaactctcg tgcctccagt ccctgccaag
     5821 aatttgaaca gtttcagatc gtcccaactg tggagacacc ctatttggcc cgagcaggga
     5881 agaatgaatt tctcaatctt gttccagaca ttgaagaagt cagagcggga tcagtggtat
     5941 ctaagaaagg atacctgcat ttcaaagagc cactttccag taactgggct aaacatttcg
     6001 ttgtggttcg tcgcccttac gtcttcatct ataacagtga cgaagaccca gtagagcgtg
     6061 gcatcattaa cctgtctaca gcacaggtgg agtacagcga ggaccagcag gccatggtga
     6121 agacacctaa tacttttgct gtatgcacaa agcaccgtgg ggtccttctg caagctctca
     6181 atgacaaaga catgaatgac tggttatatg cctttaaccc acttctggct ggcacaataa
     6241 ggtcaaaact ctctcgcaga tgcccgaacc agccaaagta ctgactctag ataagtaatg
     6301 atcataatca gccatatcac atctgtagag gttttacttg ctttaaaaaa cctcccacac
     6361 ctccccctga acctgaaaca taaaatgaat gcaattgttg ttgttaactt gtttattgca
     6421 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     6481 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggatc
     6541 cccgggtacc gagctcgaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     6601 atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     6661 cctaatgagt gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     6721 gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     6781 gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     6841 ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     6901 acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     6961 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     7021 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     7081 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     7141 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     7201 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     7261 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     7321 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     7381 tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     7441 tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     7501 ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     7561 aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     7621 aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     7681 aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     7741 gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     7801 gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     7861 caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     7921 ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     7981 attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     8041 ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     8101 gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     8161 ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     8221 tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     8281 gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     8341 cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     8401 gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     8461 tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     8521 ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     8581 gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     8641 tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     8701 catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     8761 ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     8821 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     8881 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggt tggcttaact
     8941 atgcggcatc agagcagatt gtactgagag tgcaccatat gcggtgtgaa ataccgcaca
     9001 gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc attcaggctg cgcaactgtt
     9061 gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa gggggatgtg
     9121 ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga
     9181 cggccagtgc caagttggga tctttgcatt ggcccacggc tctcaggatg gggatgctcc
     9241 ccttcagcac ccggttcccc ttggaaactg atggtcctgg ctctgtggca tggcagtggc
     9301 actgtgagga gcccctacca gcagcacaca gtgggtttgg cactgccacg ctccggatgc
     9361 cgcgctctga tccaacccca taatcaaggg aacccgaatt gccccatcat tgcccccacc
     9421 acccccatcc tgccgggccc tcacacccca cgctgccttg tggtgacatt ccccagccca
     9481 aacccacggc ttcatggcta ccgcggggca tttcccattg ccgccccatt atcagctctg
     9541 cacacctccc gctgtaccca tgcctcgtgg ctgcccttct ttgacgtata atcttctaat
     9601 taatacccgg ccttgtcaaa gtggagcaca aacgttaatt aattccccag caggcaggta
     9661 attaacagtg tgactccctt tttgctgcga gtggggctga tacagagaga tgtggcacta
     9721 tggagcccac ggggtcctgg cactgggtgc ccacggaggt ccccatgtgc tgcagtgtca
     9781 ccgcctccga ggtgacagta ttgtccctgc ggtgtccctg cagctcagct ctgtccacag
     9841 ggccacctcc agtttggagg ggacacaatg cagccccgat gcaacccatc ctcgcagcat
     9901 cccagggaca aagaccccac tgcaagaccg cacacagggc tgggtcccgc tcccctaata
     9961 tctacagtgc ttttgcatgg ccccttaatc aatgcagtta atcagcatgc gctcatgcac
    10021 cgctctggag ctgcaaagcc cctcgcagcg ctgctcacca acaccgcgca ccgccccggc
    10081 ccagcctgca gcacgcgctg caaacaggaa agaaacaaaa tattgcccaa atgtaggcaa
    10141 aggcattcgg ctgccttgac ctccgccggg ccgggccctg cctgactcag ctccttactc
    10201 agcgctcgct tcctccctcc ggctgccacc gccgcagcgc acaccctgac aaagagtggc
    10261 ccttaacggg ctctgaggtg cacccagcag tgcactcagc agtccaaggg ccggcctgga
    10321 ggtttgcacc gctacgtgct gacattagca ttgaacttgg ccctgggtag tgctgcaggc
    10381 cgggcggggt gggtgtagag agtgcagcgc gcgttgcacc cggtgcccct tcccctccct
    10441 tgcatcccag caggctgcac cccagcacca ggcccgtgca tgcatgctcc tggtgttatt
    10501 gcagcctggt gcatgcatgc gtcttagtgg tgcagcgctg tgcatgcatc ctccttggtg
    10561 tgtagcagc
//
