LOCUS       sequence-109063-        9506 bp    DNA     circular SYN 15-APR-2025
DEFINITION  generating transgenic TRIC flies.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9506)
  AUTHORS   Gao XJ, Riabinina O, Li J, Potter CJ, Clandinin TR, Luo L
  TITLE     A transcriptional reporter of intracellular Ca in Drosophila.
  JOURNAL   Nat Neurosci. 2015 May 11. doi: 10.1038/nn.4016.
  PUBMED    25961791
REFERENCE   2  (bases 1 to 9506)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2015 May 11. doi: 10.1038/nn.4016."
COMMENT     Sequence Label: pQUAST-MKII::GAL4DBDo
FEATURES             Location/Qualifiers
     source          1..9506
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(235..823)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(315..334)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(994..1785,1786..1854))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1617..1636
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1855..1959)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     2027..2045
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(2083..2105)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2205..2224
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(2761..2791,2792..2993)
                     /label=P element 3' end
                     /note="P element 3' end"
     promoter        3189..3427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     3374..3395
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
     intron          4144..4209
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             4339..4359
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4631..4765
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4668..4687)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4722..4741
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     gene            4783..8910
                     /gene="white"
                     /label=mini-white
                     /note="This modified version of the white gene lacks part 
                     of the first intron."
     primer_bind     complement(4792..4810)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(5261..5332,5727..6000,6075..6729,6791..7106,7327..7458,
                     7529..8143)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(8921..9475,9476..9506)
                     /label=P element 5' end
ORIGIN
        1 ggccagaccc acgtagtcca gcggcagatc ggcggcggag aagttaagcg tctccaggat
       61 gaccttgccc gaactggggc acgtggtgtt cgacgatgtg cagctaattt cgcccggctc
      121 cacgtccgcc cattggttaa tcagcagacc ctcgttggcg taacggaacc atgagaggta
      181 cgacaaccat ttgaggtata ctggcaccga gcccgagttc aagaagaagg cgtttttcca
      241 taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
      301 cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
      361 tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
      421 gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
      481 gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
      541 tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
      601 gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
      661 cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
      721 aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
      781 tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
      841 ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
      901 attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
      961 ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     1021 tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     1081 aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     1141 acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     1201 aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     1261 agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     1321 ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     1381 agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     1441 tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     1501 tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     1561 attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa cacgggataa
     1621 taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     1681 aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     1741 caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     1801 gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     1861 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     1921 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     1981 acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata ggcgtatcac
     2041 gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
     2101 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
     2161 cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac tatgcggcat cagagcagat
     2221 tgtactgaga gtgcaccata tgcggtgtga aataccgcac cgaatcgcgc ggaactaacg
     2281 acagtcgctc caaggtcgtc gaacaaaagg tgaatgtgtt gcggagagcg ggtgggagac
     2341 agcgaaagag caactacgaa acgtggtgtg gtggaggtga attatgaaga gggcgcgcga
     2401 tttgaaaagt atgtatataa aaaatatatc ccggtgtttt atgtagcgat aaacgagttt
     2461 ttgatgtaag gtatgcaggt gtgtaagtct tttggttaga agacaaatcc aaagtctact
     2521 tgtggggatg ttcgaagggg aaatacttgt attctatagg tcatatcttg tttttattgg
     2581 cacaaatata attacattag ctttttgagg gggcaataaa cagtaaacac gatggtaata
     2641 atggtaaaaa aaaaaacaag cagttatttc ggatatatgt cggctactcc ttgcgtcggg
     2701 cccgaagtct tagagccaga tatgcgagca cccggaagct cacgatgaga atggccagac
     2761 catgatgaaa taacataagg tggtcccgtc ggcaagagac atccacttaa cgtatgcttg
     2821 caataagtgc gagtgaaagg aatagtattc tgagtgtcgt attgagtctg agtgagacag
     2881 cgatatgatt gttgattaac ccttagcatg tccgtggggt ttgaattaac tcataatatt
     2941 aattagacga aattattttt aaagttttat ttttaataat ttgcgagtac gcaaagcttg
     3001 gctgcatcca acgcgttggg agctctccgg atccaagctt ggatccgggt aatcgcttat
     3061 cctcggataa acaattatcc tcacgggtaa tcgcttatcc gctcgggtaa tcgcttatcc
     3121 tcgggtaatc gcttatcctt aagcttgata tcgaattcct gcagcccgag cggagactct
     3181 agcgagcgcc ggagtataaa tagaggcgct tcgtctacgg agcgacaatt caattcaaac
     3241 aagcaaagtg aacacgtcgc taagcgaaag ctaagcaaat aaacaagcgc agctgaacaa
     3301 gctaaacaat ctgcagtaaa gtgcaagtta aagtgaatca attaaaagta accagcaacc
     3361 aagtaaatca actgcaacta ctgaaatctg ccaagaagta attattgaat acaagaagag
     3421 aactctgaat agggaattgg gaattcgtta acagatcggg ggatcaattc gttaacagat
     3481 ctgcggccgc accatgttta atgcgcggcg caagctaaag ggagccatac ttacgacaat
     3541 gttggcgacc agaaattttt cggctagcgg aggaggtggt ggaggtggag gtggaggtac
     3601 tagtatgaag ctgctgagta gtattgaaca agcgtgtgac atatgccgac ttaagaagct
     3661 gaagtgcagc aaggaaaagc cgaagtgcgc caaatgtctt aagaacaatt gggagtgccg
     3721 gtactcgcca aagacgaagc gaagcccttt gacacgcgct catttgactg aagtggagag
     3781 tcgcctggag cgccttgagc agttgttcct cctcattttt ccccgtgaag acctcgacat
     3841 gatactgaag atggatagtc tccaagatat taaggccctg ctcactggcc tgtttgttca
     3901 ggataacgtc aacaaggatg ccgtgacgga ccgccttgcc tcggtggaga cagatatgcc
     3961 acttaccttg cggcagcatc gcatctcggc taccagcagt agtgaagaaa gttccaataa
     4021 gggtcaacgg caactgacgg tatcgtaact cgagggtacc tctagaggat ctttgtgaag
     4081 gaaccttact tctgtggtgt gacataattg gacaaactac ctacagagat ttaaagctct
     4141 aaggtaaata taaaattttt aagtgtataa tgtgttaaac tactgattct aattgtttgt
     4201 gtattttaga ttccaaccta tggaactgat gaatgggagc agtggtggaa tgcctttaat
     4261 gaggaaaacc tgttttgctc agaagaaatg ccatctagtg atgatgaggc tactgctgac
     4321 tctcaacatt ctactcctcc aaaaaagaag agaaaggtag aagaccccaa ggactttcct
     4381 tcagaattgc taagtttttt gagtcatgct gtgtttagta atagaactct tgcttgcttt
     4441 gctatttaca ccacaaagga aaaagctgca ctgctataca agaaaattat ggaaaaatat
     4501 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     4561 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     4621 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     4681 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     4741 caatgtatct tatcatgtct ggatccacta gaaggcctaa ttctagtatg tatgtaagtt
     4801 aataaaaccc atttttgcgg aaagtagata aaaaaaacat tttttttttt tactgcactg
     4861 gatatcattg aacttatctg atcagtttta aatttacttc gatccaaggg tatttgatgt
     4921 accaggttct ttcgattacc tctcactcaa aatgacattc cactcaaagt cagcgctgtt
     4981 tgcctccttc tctgtccaca gaaatatcgc cgtctctttc gccgctgcgt ccgctatctc
     5041 tttcgccacc gtttgtagcg ttacgtagcg tcaatgtccg ccttcagttg cttttgtcag
     5101 cggtttcgtg acgaagctcc aagcggttta cgccatcaat taaacacaaa gtgctgtgcc
     5161 aaaactcctc tcgcttctta tttttgtttg ttttttgagt gattggggtg gtgattggtt
     5221 ttgggtgggt aagcagggga aagtgtgaaa aatcccggca atgggccaag aggatcagga
     5281 gctattaatt cgcggaggca gcaaacaccc atctgccgag catctgaaca atgtgagtag
     5341 tacatgtgca tacatcttaa gttcacttga tctataggaa ctgcgattgc aacatcaaat
     5401 tgtctgcggc gtgagaactg cgacccacaa aaatcccaaa ccgcaatcgc acaaacaaat
     5461 agtgacacga aacagattat tctggtagct gtgctcgcta tataagacaa tttttaagat
     5521 catatcatga tcaagacatc taaaggcatt cattttcgac tacattcttt tttacaaaaa
     5581 atataacaac cagatatttt aagctgatcc tagatgcaca aaaaataaat aaaagtataa
     5641 acctacttcg taggatactt cgttttgttc ggggttagat gagcataacg cttgtagttg
     5701 atatttgaga tcccctatca ttgcagggtg acagcggagc ggcttcgcag agctgcatta
     5761 accagggctt cgggcaggcc aaaaactacg gcacgctcct gccacccagt ccgccggagg
     5821 actccggttc agggagcggc caactagccg agaacctcac ctatgcctgg cacaatatgg
     5881 acatctttgg ggcggtcaat cagccgggct ccggatggcg gcagctggtc aaccggacac
     5941 gcggactatt ctgcaacgag cgacacatac cggcgcccag gaaacatttg ctcaagaacg
     6001 gtgagtttct attcgcagtc ggctgatctg tgtgaaatct taataaaggg tccaattacc
     6061 aatttgaaac tcagtttgcg gcgtggccta tccgggcgaa cttttggccg tgatgggcag
     6121 ttccggtgcc ggaaagacga ccctgctgaa tgcccttgcc tttcgatcgc cgcagggcat
     6181 ccaagtatcg ccatccggga tgcgactgct caatggccaa cctgtggacg ccaaggagat
     6241 gcaggccagg tgcgcctatg tccagcagga tgacctcttt atcggctccc taacggccag
     6301 ggaacacctg attttccagg ccatggtgcg gatgccacga catctgacct atcggcagcg
     6361 agtggcccgc gtggatcagg tgatccagga gctttcgctc agcaaatgtc agcacacgat
     6421 catcggtgtg cccggcaggg tgaaaggtct gtccggcgga gaaaggaagc gtctggcatt
     6481 cgcctccgag gcactaaccg atccgccgct tctgatctgc gatgagccca cctccggact
     6541 ggactcattt accgcccaca gcgtcgtcca ggtgctgaag aagctgtcgc agaagggcaa
     6601 gaccgtcatc ctgaccattc atcagccgtc ttccgagctg tttgagctct ttgacaagat
     6661 ccttctgatg gccgagggca gggtagcttt cttgggcact cccagcgaag ccgtcgactt
     6721 cttttcctag tgagttcgat gtgtttatta agggtatcta gcattacatt acatctcaac
     6781 tcctatccag cgtgggtgcc cagtgtccta ccaactacaa tccggcggac ttttacgtac
     6841 aggtgttggc cgttgtgccc ggacgggaga tcgagtcccg tgatcggatc gccaagatat
     6901 gcgacaattt tgctattagc aaagtagccc gggatatgga gcagttgttg gccaccaaaa
     6961 atttggagaa gccactggag cagccggaga atgggtacac ctacaaggcc acctggttca
     7021 tgcagttccg ggcggtcctg tggcgatcct ggctgtcggt gctcaaggaa ccactcctcg
     7081 taaaagtgcg acttattcag acaacggtga gtggttccag tggaaacaaa tgatataacg
     7141 cttacaattc ttggaaacaa attcgctaga ttttagttag aattgcctga ttccacaccc
     7201 ttcttagttt ttttcaatga gatgtatagt ttatagtttt gcagaaaata aataaatttc
     7261 atttaactcg cgaacatgtt gaagatatga atattaatga gatgcgagta acattttaat
     7321 ttgcagatgg ttgccatctt gattggcctc atctttttgg gccaacaact cacgcaagtg
     7381 ggcgtgatga atatcaacgg agccatcttc ctcttcctga ccaacatgac ctttcaaaac
     7441 gtctttgcca cgataaatgt aagtcttgtt tagaatacat ttgcatatta ataatttact
     7501 aactttctaa tgaatcgatt cgatttaggt gttcacctca gagctgccag tttttatgag
     7561 ggaggcccga agtcgacttt atcgctgtga cacatacttt ctgggcaaaa cgattgccga
     7621 attaccgctt tttctcacag tgccactggt cttcacggcg attgcctatc cgatgatcgg
     7681 actgcgggcc ggagtgctgc acttcttcaa ctgcctggcg ctggtcactc tggtggccaa
     7741 tgtgtcaacg tccttcggat atctaatatc ctgcgccagc tcctcgacct cgatggcgct
     7801 gtctgtgggt ccgccggtta tcataccatt cctgctcttt ggcggcttct tcttgaactc
     7861 gggctcggtg ccagtatacc tcaaatggtt gtcgtacctc tcatggttcc gttacgccaa
     7921 cgagggtctg ctgattaacc aatgggcgga cgtggagccg ggcgaaatta gctgcacatc
     7981 gtcgaacacc acgtgcccca gttcgggcaa ggtcatcctg gagacgctta acttctccgc
     8041 cgccgatctg ccgctggact acgtgggtct ggccattctc atcgtgagct tccgggtgct
     8101 cgcatatctg gctctaagac ttcgggcccg acgcaaggag tagccgacat atatccgaaa
     8161 taactgcttg tttttttttt taccattatt accatcgtgt ttactgttta ttgccccctc
     8221 aaaaagctaa tgtaattata tttgtgccaa taaaaacaag atatgaccta tagaatacaa
     8281 gtatttcccc ttcgaacatc cccacaagta gactttggat ttgtcttcta accaaaagac
     8341 ttacacacct gcatacctta catcaaaaac tcgtttatcg ctacataaaa caccgggata
     8401 tattttttat atacatactt ttcaaatcgc gcgccctctt cataattcac ctccaccaca
     8461 ccacgtttcg tagttgctct ttcgctgtct cccacccgct ctccgcaaca cattcacctt
     8521 ttgttcgacg accttggagc gactgtcgtt agttccgcgc gattcggttc gctcaaatgg
     8581 ttccgagtgg ttcatttcgt ctcaatagaa attagtaata aatatttgta tgtacaattt
     8641 atttgctcca atatatttgt atatatttcc ctcacagcta tatttattct aatttaatat
     8701 tatgactttt taaggtaatt ttttgtgacc tgttcggagt gattagcgtt acaatttgaa
     8761 ctgaaagtga catccagtgt ttgttccttg tgtagatgca tctcaaaaaa atggtgggca
     8821 taatagtgtt gtttatatat atcaaaaata acaactataa taataagaat acatttaatt
     8881 tagaaaatgc ttggatttca ctggaactag aattaattcg gctgctgctc taaacgacgc
     8941 atttcgtact ccaaagtacg aattttttcc ctcaagctct tattttcatt aaacaatgaa
     9001 caggacctaa cgcacagtca cgttattgtt tacataaatg atttttttta ctattcaaac
     9061 ttactctgtt tgtgtactcc cactggtata gccttctttt atcttttctg gttcaggctc
     9121 tatcacttta ctaggtacgg catctgcgtt gagtcgcctc cttttaaatg tctgaccttt
     9181 tgcaggtgca gccttccact gcgaatcatt aaagtgggta tcacaaattt gggagttttc
     9241 accaaggctg cacccaaggc tctgctccca caattttctc ttaatagcac acttcggcac
     9301 gtgaattaat tttactccag tcacagcttt gcagcaaaat ttgcaatatt tcattttttt
     9361 ttattccacg taagggttaa tgttttcaaa aaaaaattcg tccgcacaca acctttcctc
     9421 tcaacaagca aacgtgcact gaatttaagt gtatacttcg gtaagcttcg gctatcgacg
     9481 ggaccacctt atgttatttc atcatg
//
