LOCUS       sequence-109319-        9163 bp    DNA     circular SYN 14-APR-2025
DEFINITION  A lentiviral vector for the expression of mouse Sox10 under 
            doxycyline control. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9163)
  AUTHORS   Najm FJ, Lager AM, Zaremba A, Wyatt K, Caprariello AV, Factor DC, 
            Karl RT, Maeda T, Miller RH, Tesar PJ
  TITLE     Transcription factor-mediated reprogramming of fibroblasts to 
            expandable, myelinogenic oligodendrocyte progenitor cells.
  JOURNAL   Nat Biotechnol. 2013 May;31(5):426-33. doi: 10.1038/nbt.2561. Epub 
            2013 Apr 14.
  PUBMED    23584611
REFERENCE   2  (bases 1 to 9163)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.2561"; 
            journalName: "Nat Biotechnol"; date: "2013-05"; volume: "31"; issue:
            "5"; pages: "426-33"
COMMENT     Sequence Label: pLVX-Tight-Puro_Sox10
FEATURES             Location/Qualifiers
     source          1..9163
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             1..634
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    681..806
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1303..1536
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1721..1765
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2028..2143
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        2206..2520
                     /label=tight TRE promoter
                     /note="Tet-responsive promoter PTight, consisting of seven 
                     tet operator sequences followed by the minimal CMV 
                     promoter"
     protein_bind    2214..2232
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2250..2268
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2285..2303
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2321..2339
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2357..2375
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2392..2410
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    2428..2446
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     2493..2517
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     promoter        3948..4447
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(3983..4006)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     4380..4399
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             4468..5067
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4468..4487)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4964..4984
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5085..5673
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5138..5158)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5556..5567)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5881..6514
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6643..6659)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6643..6659)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6656..6678)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6667..6683
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6691..6697,6698..6715,6716..6721))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6736..6757
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6874..6891)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7045..7630)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7125..7144)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7801..8592,8593..8661))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8424..8443
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8662..8766)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    8814..8948
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8851..8870)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8905..8924
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga actacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaagga tccaccatgg ccgaggaaca agacctatca gaggtggagc tgagccctgt
     2581 gggctcggag gaaccccgct gcctgtcccc aggcagcgcg ccgtcgctgg gacccgacgg
     2641 cggcggcggt ggctcgggct tgcgagccag cccggggccc ggtgaactgg gcaaggtcaa
     2701 gaaggaacag caggacggcg aggcggacga tgacaagttc cccgtgtgca tccgcgaggc
     2761 ggtcagccag gtgctcagcg gctacgactg gacgctggtg cccatgcccg tgcgcgtcaa
     2821 cggtgccagc aagagcaagc cgcacgtcaa gaggcccatg aacgccttca tggtgtgggc
     2881 acaggcggca cgcagaaagc tagccgacca gtaccctcac ctccacaatg ctgagctcag
     2941 caagacacta ggcaagctct ggaggttgct gaacgaaagt gacaagcgcc ccttcattga
     3001 ggaggctgag cggctccgga tgcagcacaa aaaggaccat ccggactaca agtaccaacc
     3061 tcggcggcgg aagaacggga aggcagccca gggggaggca gaatgcccag gcggggaagc
     3121 cgagcaagga ggggctgctg ctattcaggc tcactacaag agtgcccacc tggaccaccg
     3181 gcacccagaa gaaggctccc ccatgtcaga tgggaaccca gagcacccct caggccagag
     3241 ccatggcccc ccaacccctc caaccacccc aaagacagag ctgcagtccg gcaaggcaga
     3301 ccccaaaagg gatgggcgct ccttggggga gggcgggaag ccccacatcg acttcggcaa
     3361 cgtggacatc ggggagatca gccacgaggt aatgtccaac atggaaacct ttgatgtgac
     3421 tgagctggac caatacctgc cacccaacgg gcacccaggc catgtgggta gctactcggc
     3481 agctggctat gggctgggca gtgccctggc tgtggccagt ggacactctg cctggatctc
     3541 caagccacca ggtgtggctc tgcccacggt ctcgccccct ggtgtggatg ccaaagccca
     3601 ggtgaagaca gagaccacag gcccccaggg acccccacac tacaccgacc agccgtccac
     3661 ttcccagatc gcctacacct ccctcagtct gccccactac ggctccgcct tcccctccat
     3721 ctcacgaccc cagtttgact attctgacca tcagccctca ggaccctatt atggccatgc
     3781 aggccaggcc tctggcctct attcagcctt ttcttacatg gggccctccc agcggcccct
     3841 ctacactgcc atctctgacc ccagcccctc ggggccccaa tcccacagtc ccacacactg
     3901 ggagcagcca gtatatacga ctctatcccg accttaggaa ttctaccggg taggggaggc
     3961 gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta
     4021 cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc
     4081 gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc
     4141 cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct
     4201 cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca
     4261 atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg
     4321 cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc
     4381 attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg
     4441 cctttcgacc tgcagcccaa gcttaccatg accgagtaca agcccacggt gcgcctcgcc
     4501 acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc cgactacccc
     4561 gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga gctgcaagaa
     4621 ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc
     4681 gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc
     4741 ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca gatggaaggc
     4801 ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt cggcgtctcg
     4861 cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc
     4921 gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct ccccttctac
     4981 gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg
     5041 tgcatgaccc gcaagcccgg tgcctgacgg ggcgcgtctg gaacaatcaa cctctggatt
     5101 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
     5161 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
     5221 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
     5281 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
     5341 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
     5401 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
     5461 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
     5521 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
     5581 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
     5641 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct
     5701 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
     5761 gctagaagca caagaggagg aggaggtggg tttttccagt cacacctcag gtacctttaa
     5821 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aagaggggac
     5881 tggaagggct aattcactcc caacgaagac aagatatcct tgatctgtgg atctaccaca
     5941 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     6001 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     6061 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     6121 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     6181 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     6241 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     6301 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     6361 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     6421 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     6481 agaccctttt agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt
     6541 cagtatttat aacttgcaaa gaaatgaata tcagagagtg agaggccttg acattgctag
     6601 cgttttaccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
     6661 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     6721 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     6781 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     6841 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     6901 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     6961 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     7021 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     7081 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     7141 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     7201 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     7261 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     7321 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     7381 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     7441 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     7501 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     7561 acaaaccacc gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa
     7621 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa
     7681 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt
     7741 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag
     7801 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat
     7861 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc
     7921 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa
     7981 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca
     8041 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa
     8101 cgttgttgcc attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt
     8161 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc
     8221 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact
     8281 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc
     8341 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg
     8401 ctcttgcccg gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct
     8461 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc
     8521 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag
     8581 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac
     8641 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg
     8701 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt
     8761 tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac ggatcgggag atcaacttgt
     8821 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     8881 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     8941 tctggatcaa ctggataact caagctaacc aaaatcatcc caaacttccc accccatacc
     9001 ctattaccac tgccaattac ctagtggttt catttactct aaacctgtga ttcctctgaa
     9061 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttttt
     9121 aaagaaattg tatttgttaa atatgtacta caaacttagt agt
//
