LOCUS       sequence-109864-       10353 bp    DNA     circular SYN 14-APR-2025
DEFINITION  for expression of cytosolic roGFP2-Orp1 with the Gal4 expression 
            system in drosophila.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10353)
  AUTHORS   Albrecht SC, Barata AG, Grosshans J, Teleman AA, Dick TP
  TITLE     In vivo mapping of hydrogen peroxide and oxidized glutathione 
            reveals chemical and regional specificity of redox homeostasis.
  JOURNAL   Cell Metab. 2011 Dec 7;14(6):819-29. doi: 
            10.1016/j.cmet.2011.10.010. Epub 2011 Nov 17.
  PUBMED    22100409
REFERENCE   2  (bases 1 to 10353)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2011.10.010"; journalName: "Cell Metab"; date: 
            "2011-12-7- 7"; volume: "14"; issue: "6"; pages: "819-29"
COMMENT     Sequence Label: pUAST cyto roGFP2-Orp1
FEATURES             Location/Qualifiers
     source          1..10353
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(27..29,30..32,33..743)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFISTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNCHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTCSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(72..93)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(333..352)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     680..701
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     intron          1424..1489
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             1619..1639
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    2064..2198
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2101..2120)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2155..2174
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2225..2243)
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     CDS             join(2703..2774,3169..3442,3517..4171,4233..4548,4769..4900,
                     4971..5585)
                     /codon_start=1
                     /gene="white"
                     /product="Drosophila white gene eye color pigment"
                     /label=mini-white
                     /note="This is a modified version of the white gene lacking
                     part of the first intron."
                     /translation="MGQEDQELLIRGGSKHPSAEHLNNGDSGAASQSCINQGFGQAKNY
                     GTLLPPSPPEDSGSGSGQLAENLTYAWHNMDIFGAVNQPGSGWRQLVNRTRGLFCNERH
                     IPAPRKHLLKNVCGVAYPGELLAVMGSSGAGKTTLLNALAFRSPQGIQVSPSGMRLLNG
                     QPVDAKEMQARCAYVQQDDLFIGSLTAREHLIFQAMVRMPRHLTYRQRVARVDQVIQEL
                     SLSKCQHTIIGVPGRVKGLSGGERKRLAFASEALTDPPLLICDEPTSGLDSFTAHSVVQ
                     VLKKLSQKGKTVILTIHQPSSELFELFDKILLMAEGRVAFLGTPSEAVDFFSYVGAQCP
                     TNYNPADFYVQVLAVVPGREIESRDRIAKICDNFAISKVARDMEQLLATKNLEKPLEQP
                     ENGYTYKATWFMQFRAVLWRSWLSVLKEPLLVKVRLIQTTMVAILIGLIFLGQQLTQVG
                     VMNINGAIFLFLTNMTFQNVFATINVFTSELPVFMREARSRLYRCDTYFLGKTIAELPL
                     FLTVPLVFTAIAYPMIGLRAGVLHFFNCLALVTLVANVSTSFGYLISCASSSTSMALSV
                     GPPVIIPFLLFGGFFLNSGSVPVYLKWLSYLSWFRYANEGLLINQWADVEPGEISCTSS
                     NTTCPSSGKVILETLNFSAADLPLDYVGLAILIVSFRVLAYLALRLRARRKE"
     misc_feature    join(6363..6917,6918..6948)
                     /label=P element 5' end
     rep_origin      complement(7183..7771)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7263..7282)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7942..8733,8734..8802))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8565..8584
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8803..8907)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8975..8993
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(9031..9053)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     9153..9172
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    join(9709..9739,9740..9941)
                     /label=P element 3' end
                     /note="P element 3' end"
     protein_bind    9989..10083
                     /label=5X UAS
                     /bound_moiety="GAL4"
                     /note="five tandem copies of the ""ScaI site"" 17-mer 
                     CGGAGTACTGTCCTCCG, an upstream activating sequence (UAS) 
                     that efficiently binds yeast Gal4 (Webster et al., 1988; 
                     Pfeiffer et al., 2010)"
     promoter        10102..10340
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     10287..10308
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 gaattcgtta acagatctgc ggccgcatgg tgagcaaggg cgaggagctg ttcaccgggg
       61 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
      121 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tccaccaccg
      181 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
      241 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
      301 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
      361 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
      421 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaactgc cacaacgtct
      481 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
      541 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
      601 gccccgtgct gctgcccgac aaccactacc tgagcacctg ctccgccctg agcaaagacc
      661 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc
      721 tcggcatgga cgagctgtac aagactagtg gtggttcagg tggtggtggt tcaggtggtg
      781 gtggttcagg tggaggagga tcaggaggag gaggatcagg aggaggagga tcaggaggag
      841 aattcgatat ctcagaattc tataagctag cacctgttga caagaaaggc caaccattcc
      901 ccttcgacca attaaaggga aaagtggtgc ttatcgttaa tgttgcctcc aaatgtggat
      961 tcactcctca atacaaagaa ctagaggcct tgtacaaacg ttataaggac gaaggattta
     1021 ccatcatcgg gttcccatgc aaccagtttg gccaccaaga acctggctct gatgaagaaa
     1081 ttgcccagtt ctgccaactg aactatggcg tgactttccc cattatgaaa aaaattgacg
     1141 ttaatggtgg caatgaggac cctgtttaca agtttttgaa gagccaaaaa tccggtatgt
     1201 tgggcttgag aggtatcaaa tggaattttg aaaaattctt agtcgataaa aagggtaaag
     1261 tgtacgaaag atactcttca ctaaccaaac cttcttcgtt gtccgaaacc atcgaagaac
     1321 ttttgaaaga ggtggaatag tctagaggat ctttgtgaag gaaccttact tctgtggtgt
     1381 gacataattg gacaaactac ctacagagat ttaaagctct aaggtaaata taaaattttt
     1441 aagtgtataa tgtgttaaac tactgattct aattgtttgt gtattttaga ttccaaccta
     1501 tggaactgat gaatgggagc agtggtggaa tgcctttaat gaggaaaacc tgttttgctc
     1561 agaagaaatg ccatctagtg atgatgaggc tactgctgac tctcaacatt ctactcctcc
     1621 aaaaaagaag agaaaggtag aagaccccaa ggactttcct tcagaattgc taagtttttt
     1681 gagtcatgct gtgtttagta atagaactct tgcttgcttt gctatttaca ccacaaagga
     1741 aaaagctgca ctgctataca agaaaattat ggaaaaatat tctgtaacct ttataagtag
     1801 gcataacagt tataatcata acatactgtt ttttcttact ccacacaggc atagagtgtc
     1861 tgctattaat aactatgctc aaaaattgtg tacctttagc tttttaattt gtaaaggggt
     1921 taataaggaa tatttgatgt atagtgcctt gactagagat cataatcagc cataccacat
     1981 ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata
     2041 aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa
     2101 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     2161 tgtccaaact catcaatgta tcttatcatg tctggatcgg atccactaga aggccttagt
     2221 atgtatgtaa gttaataaaa cccttttttg gagaatgtag atttaaaaaa acatattttt
     2281 tttttatttt ttactgcact ggacatcatt gaacttatct gatcagtttt aaatttactt
     2341 cgatccaagg gtatttgaag taccaggttc tttcgattac ctctcactca aaatgacatt
     2401 ccactcaaag tcagcgctgt ttgcctcctt ctctgtccac agaaatatcg ccgtctcttt
     2461 cgccgctgcg tccgctatct ctttcgccac cgtttgtagc gttacctagc gtcaatgtcc
     2521 gccttcagtt gcactttgtc agcggtttcg tgacgaagct ccaagcggtt tacgccatca
     2581 attaaacaca aagtgctgtg ccaaaactcc tctcgcttct tatttttgtt tgttttttga
     2641 gtgattgggg tggtgattgg ttttgggtgg gtaagcaggg gaaagtgtga aaaatcccgg
     2701 caatgggcca agaggatcag gagctattaa ttcgcggagg cagcaaacac ccatctgccg
     2761 agcatctgaa caatgtgagt agtacatgtg catacatctt aagttcactt gatctatagg
     2821 aactgcgatt gcaacatcaa attgtctgcg gcgtgagaac tgcgacccac aaaaatccca
     2881 aaccgcaatc gcacaaacaa atagtgacac gaaacagatt attctggtag ctgtgctcgc
     2941 tatataagac aatttttaag atcatatcat gatcaagaca tctaaaggca ttcattttcg
     3001 actacattct tttttacaaa aaatataaca accagatatt ttaagctgat cctagatgca
     3061 caaaaaataa ataaaagtat aaacctactt cgtaggatac ttcgttttgt tcggggttag
     3121 atgagcataa cgcttgtagt tgatatttga gatcccctat cattgcaggg tgacagcgga
     3181 gcggcttcgc agagctgcat taaccagggc ttcgggcagg ccaaaaacta cggcacgctc
     3241 ctgccaccca gtccgccgga ggactccggt tcagggagcg gccaactagc cgagaacctc
     3301 acctatgcct ggcacaatat ggacatcttt ggggcggtca atcagccggg ctccggatgg
     3361 cggcagctgg tcaaccggac acgcggacta ttctgcaacg agcgacacat accggcgccc
     3421 aggaaacatt tgctcaagaa cggtgagttt ctattcgcag tcggctgatc tgtgtgaaat
     3481 cttaataaag ggtccaatta ccaatttgaa actcagtttg cggcgtggcc tatccgggcg
     3541 aacttttggc cgtgatgggc agttccggtg ccggaaagac gaccctgctg aatgcccttg
     3601 cctttcgatc gccgcagggc atccaagtat cgccatccgg gatgcgactg ctcaatggcc
     3661 aacctgtgga cgccaaggag atgcaggcca ggtgcgccta tgtccagcag gatgacctct
     3721 ttatcggctc cctaacggcc agggaacacc tgattttcca ggccatggtg cggatgccac
     3781 gacatctgac ctatcggcag cgagtggccc gcgtggatca ggtgatccag gagctttcgc
     3841 tcagcaaatg tcagcacacg atcatcggtg tgcccggcag ggtgaaaggt ctgtccggcg
     3901 gagaaaggaa gcgtctggca ttcgcctccg aggcactaac cgatccgccg cttctgatct
     3961 gcgatgagcc cacctccgga ctggactcat ttaccgccca cagcgtcgtc caggtgctga
     4021 agaagctgtc gcagaagggc aagaccgtca tcctgaccat tcatcagccg tcttccgagc
     4081 tgtttgagct ctttgacaag atccttctga tggccgaggg cagggtagct ttcttgggca
     4141 ctcccagcga agccgtcgac ttcttttcct agtgagttcg atgtgtttat taagggtatc
     4201 tagcattaca ttacatctca actcctatcc agcgtgggtg cccagtgtcc taccaactac
     4261 aatccggcgg acttttacgt acaggtgttg gccgttgtgc ccggacggga gatcgagtcc
     4321 cgtgatcgga tcgccaagat atgcgacaat tttgctatta gcaaagtagc ccgggatatg
     4381 gagcagttgt tggccaccaa aaatttggag aagccactgg agcagccgga gaatgggtac
     4441 acctacaagg ccacctggtt catgcagttc cgggcggtcc tgtggcgatc ctggctgtcg
     4501 gtgctcaagg aaccactcct cgtaaaagtg cgacttattc agacaacggt gagtggttcc
     4561 agtggaaaca aatgatataa cgcttacaat tcttggaaac aaattcgcta gattttagtt
     4621 agaattgcct gattccacac ccttcttagt ttttttcaat gagatgtata gtttatagtt
     4681 ttgcagaaaa taaataaatt tcatttaact cgcgaacatg ttgaagatat gaatattaat
     4741 gagatgcgag taacatttta atttgcagat ggttgccatc ttgattggcc tcatcttttt
     4801 gggccaacaa ctcacgcaag tgggcgtgat gaatatcaac ggagccatct tcctcttcct
     4861 gaccaacatg acctttcaaa acgtctttgc cacgataaat gtaagtcttg tttagaatac
     4921 atttgcatat taataattta ctaactttct aatgaatcga ttcgatttag gtgttcacct
     4981 cagagctgcc agtttttatg agggaggccc gaagtcgact ttatcgctgt gacacatact
     5041 ttctgggcaa aacgattgcc gaattaccgc tttttctcac agtgccactg gtcttcacgg
     5101 cgattgccta tccgatgatc ggactgcggg ccggagtgct gcacttcttc aactgcctgg
     5161 cgctggtcac tctggtggcc aatgtgtcaa cgtccttcgg atatctaata tcctgcgcca
     5221 gctcctcgac ctcgatggcg ctgtctgtgg gtccgccggt tatcatacca ttcctgctct
     5281 ttggcggctt cttcttgaac tcgggctcgg tgccagtata cctcaaatgg ttgtcgtacc
     5341 tctcatggtt ccgttacgcc aacgagggtc tgctgattaa ccaatgggcg gacgtggagc
     5401 cgggcgaaat tagctgcaca tcgtcgaaca ccacgtgccc cagttcgggc aaggtcatcc
     5461 tggagacgct taacttctcc gccgccgatc tgccgctgga ctacgtgggt ctggccattc
     5521 tcatcgtgag cttccgggtg ctcgcatatc tggctctaag acttcgggcc cgacgcaagg
     5581 agtagccgac atatatccga aataactgct tgtttttttt tttaccatta ttaccatcgt
     5641 gtttactgtt tattgccccc tcaaaaagct aatgtaatta tatttgtgcc aataaaaaca
     5701 agatatgacc tatagaatac aagtatttcc ccttcgaaca tccccacaag tagactttgg
     5761 atttgtcttc taaccaaaag acttacacac ctgcatacct tacatcaaaa actcgtttat
     5821 cgctacataa aacaccggga tatatttttt atatacatac ttttcaaatc gcgcgccctc
     5881 ttcataattc acctccacca caccacgttt cgtagttgct ctttcgctgt ctcccacccg
     5941 ctctccgcaa cacattcacc ttttgttcga cgaccttgga gcgactgtcg ttagttccgc
     6001 gcgattcggt tcgctcaaat ggttccgagt ggttcatttc gtctcaatag aaattagtaa
     6061 taaatatttg tatgtacaat ttatttgctc caatatattt gtatatattt ccctcacagc
     6121 tatatttatt ctaatttaat attatgactt tttaaggtaa ttttttgtga cctgttcgga
     6181 gtgattagcg ttacaatttg aactgaaagt gacatccagt gtttgttcct tgtgtagatg
     6241 catctcaaaa aaatggtggg cataatagtg ttgtttatat atatcaaaaa taacaactat
     6301 aataataaga atacatttaa tttagaaaat gcttggattt cactggaact agaattaatt
     6361 cggctgctgc tctaaacgac gcatttcgta ctccaaagta cgaatttttt ccctcaagct
     6421 cttattttca ttaaacaatg aacaggacct aacgcacagt cacgttattg tttacataaa
     6481 tgattttttt tactattcaa acttactctg tttgtgtact cccactggta tagccttctt
     6541 ttatcttttc tggttcaggc tctatcactt tactaggtac ggcatctgcg ttgagtcgcc
     6601 tccttttaaa tgtctgacct tttgcaggtg cagccttcca ctgcgaatca ttaaagtggg
     6661 tatcacaaat ttgggagttt tcaccaaggc tgcacccaag gctctgctcc cacaattttc
     6721 tcttaatagc acacttcggc acgtgaatta attttactcc agtcacagct ttgcagcaaa
     6781 atttgcaata tttcattttt ttttattcca cgtaagggtt aatgttttca aaaaaaaatt
     6841 cgtccgcaca caacctttcc tctcaacaag caaacgtgca ctgaatttaa gtgtatactt
     6901 cggtaagctt cggctatcga cgggaccacc ttatgttatt tcatcatggg ccagacccac
     6961 gtagtccagc ggcagatcgg cggcggagaa gttaagcgtc tccaggatga ccttgcccga
     7021 actggggcac gtggtgttcg acgatgtgca gctaatttcg cccggctcca cgtccgccca
     7081 ttggttaatc agcagaccct cgttggcgta acggaaccat gagaggtacg acaaccattt
     7141 gaggtatact ggcaccgagc ccgagttcaa gaagaaggcg tttttccata ggctccgccc
     7201 ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact
     7261 ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct
     7321 gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcaatg
     7381 ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca
     7441 cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa
     7501 cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc
     7561 gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag
     7621 aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg
     7681 tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca
     7741 gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc
     7801 tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag
     7861 gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata
     7921 tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat
     7981 ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg
     8041 ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc
     8101 tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc
     8161 aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc
     8221 gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc
     8281 gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc
     8341 ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa
     8401 gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat
     8461 gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata
     8521 gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata ccgcgccaca
     8581 tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag
     8641 gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc
     8701 agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc
     8761 aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata
     8821 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta
     8881 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta
     8941 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg
     9001 tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac atgcagctcc cggagacggt
     9061 cacagcttgt ctgtaagcgg atgccgggag cagacaagcc cgtcagggcg cgtcagcggg
     9121 tgttggcggg tgtcggggct ggcttaacta tgcggcatca gagcagattg tactgagagt
     9181 gcaccatatg cggtgtgaaa taccgcaccg aatcgcgcgg aactaacgac agtcgctcca
     9241 aggtcgtcga acaaaaggtg aatgtgttgc ggagagcggg tgggagacag cgaaagagca
     9301 actacgaaac gtggtgtggt ggaggtgaat tatgaagagg gcgcgcgatt tgaaaagtat
     9361 gtatataaaa aatatatccc ggtgttttat gtagcgataa acgagttttt gatgtaaggt
     9421 atgcaggtgt gtaagtcttt tggttagaag acaaatccaa agtctacttg tggggatgtt
     9481 cgaaggggaa atacttgtat tctataggtc atatcttgtt tttattggca caaatataat
     9541 tacattagct ttttgagggg gcaataaaca gtaaacacga tggtaataat ggtaaaaaaa
     9601 aaaacaagca gttatttcgg atatatgtcg gctactcctt gcgtcgggcc cgaagtctta
     9661 gagccagata tgcgagcacc cggaagctca cgatgagaat ggccagacca tgatgaaata
     9721 acataaggtg gtcccgtcgg caagagacat ccacttaacg tatgcttgca ataagtgcga
     9781 gtgaaaggaa tagtattctg agtgtcgtat tgagtctgag tgagacagcg atatgattgt
     9841 tgattaaccc ttagcatgtc cgtggggttt gaattaactc ataatattaa ttagacgaaa
     9901 ttatttttaa agttttattt ttaataattt gcgagtacgc aaagcttggg ctgcatctct
     9961 ccggatccaa gcttgcatgc ctgcaggtcg gagtactgtc ctccgagcgg agtactgtcc
    10021 tccgagcgga gtactgtcct ccgagcggag tactgtcctc cgagcggagt actgtcctcc
    10081 gagcggagac tctagcgagc gccggagtat aaatagaggc gcttcgtcta cggagcgaca
    10141 attcaattca aacaagcaaa gtgaacacgt cgctaagcga aagctaagca aataaacaag
    10201 cgcagctgaa caagctaaac aatctgcagt aaagtgcaag ttaaagtgaa tcaattaaaa
    10261 gtaaccagca accaagtaaa tcaactgcaa ctactgaaat ctgccaagaa gtaattattg
    10321 aatacaagaa gagaactctg aatagggaat tgg
//
