LOCUS       sequence-110153-         887 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Vector to create a cassette for homologous recombination in S. 
            cerevisiae; adds a C-terminal tDimer2 Strep-tag II followed by HIS5 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Rai J, Pemmasani JK, Voronovsky A, Jensen IS, Manavalan A, Nyengaard
            JR, Golas MM, Sander B
  TITLE     Strep-tag II and Twin-Strep Based Cassettes for Protein Tagging by 
            Homologous Recombination and Characterization of Endogenous 
            Macromolecular Assemblies in Saccharomyces cerevisiae.
  JOURNAL   Mol Biotechnol. 2014 Jun 27.
  PUBMED    24969434
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol 
            Biotechnol. 2014 Jun 27."
COMMENT     Sequence Label: pGS1613
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(72..95)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(105..776)
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGTLIYKVKFRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FL"
     primer_bind     complement(164..187)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(362..381)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     579..599
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     631..649
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 acttatttaa taataaaaat cataaatcat aagaaattcg cttatttaga agtggcgcgc
       61 gcggtacctt atttttcgaa ttgtggatga ctccaagcac tacccaggaa caggtggtgg
      121 cggccctcgg agcgctcgta ctgttccacg atggtgtagt cctcgttgtg ggaggtgatg
      181 tccagcttgg tgtccacgta gtagtagccg ggcagctgca cgggcttctt ggccatgtag
      241 atggtcttga actccaccag gtagtggccg ccgtccttca gcttcagggc ctggtggatc
      301 tcgcccttca gcacgccgtc gcgggggtac aggcgctcgg tggaggcctc ccagcccatg
      361 gtcttcttct gcattacggg gccgtcgggg gggaagttgg tgccgcggaa cttcaccttg
      421 tagatcagcg tgccgtcctg cagggaggag tcctgggtca cggtcaccac gccgccgtcc
      481 tcgaagttca tcacgcgctc ccacttgaag ccctcgggga aggacagctt cttgtagtcg
      541 gggatgtcgg cggggtgctt cacgtacgcc ttggagccgt actggaactg gggggacagg
      601 atgtcccagg cgaagggcag ggggccgccc ttggtcacct tcagcttggc ggtctgggtg
      661 ccctcgtagg ggcggccctc gccctcgccc tcgatctcga actcgtggcc gttcacggag
      721 ccctccatgc gcaccttgaa gcgcatgaac tctttgatga cgtcctcgga ggaggcggtg
      781 ccggagctgc cgctgccggt gctgccggtg ccatgcccca ggaacaggtg gtggcggccc
      841 tcggagcgct cgtactgttc cacgatggtg tagtcctcgt tgtggga
//
