LOCUS       sequence-110154-        1005 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Expression vector for solubility screening of proteins or protein 
            fragments using a split-GFP assay..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1005)
  AUTHORS   Bulloch EM, Kingston RL
  TITLE     Identifying protein domains by global analysis of soluble fragment 
            data.
  JOURNAL   Anal Biochem. 2014 Jul 10. pii: S0003-2697(14)00280-2. doi: 
            10.1016/j.ab.2014.06.021.
  PUBMED    25016192
REFERENCE   2  (bases 1 to 1005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Anal 
            Biochem. 2014 Jul 10. pii: S0003-2697(14)00280-2. doi: 
            10.1016/j.ab.2014.06.021."
COMMENT     Sequence Label: pBAD_GFP11_T7LysH17A
FEATURES             Location/Qualifiers
     source          1..1005
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(159..192)
                     /label=T7 Phi-3.8 promoter
                     /note="weak promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(190..645)
                     /codon_start=1
                     /product="lysozyme from bacteriophage T7"
                     /label=T7 lysozyme
                     /note="inhibitor of T7 RNA polymerase"
                     /translation="MARVQFKQRESTDAIFVACSATKPSQNVGVREIRQWHKEQGWLDV
                     GYHFIIKRDGTVEAGRDEMAVGSHAKGYNHNSIGVCLVGGIDDKGKFDANFTPAQMQSL
                     RSLLVTLLAKYEGAVLRAHHEVAPKACPSFDLKRWWEKNELVTSDRG"
ORIGIN
        1 cgaaacgcgc gaggcagcag atcaattcgc gcgcgaaggc gaagcggcat gcggcccatt
       61 ggctgcctcc cacacttgga tatgcctcct cggagcctta tagaattgtt tataagactt
      121 gcgcattatt tgacctccaa tgcgaacaaa gggaaaccgc tgtggtctcc ctttagtgag
      181 ttcaattaat tatccacggt cagaagtgac cagttcgttc ttctcccacc aacgcttaag
      241 gtcgaacgaa gggcaagcct tcggcgccac ctcatgatgg gcgcgaagca cagcgccttc
      301 gtacttagcc agcagtgtga caagcagtga gcgaagggat tgcatttggg ctggcgtaaa
      361 gttagcgtcg aacttacctt tatcgtcgat accaccaaca aggcagacgc cgatagagtt
      421 gtggttgtaa cccttagcgt gagagcctac agccatctca tctcgtcctg cctccacagt
      481 accgtctcgc ttgatgataa agtggtatcc cacatcgagc caaccctgct ctttgtgcca
      541 ctggcgaatc tcacggacac caacattctg acttggcttg gtagccgagc aggcaacaaa
      601 gattgcgtca gtagattcac gttgtttaaa ctgtacacga gccattattt ctttcctcct
      661 ttccttttta atctatcaaa ggggacccgc atgcataatg tgcctgtcaa atggacgaag
      721 cagggattct gcaaacccta tgctactccg tcaagccgtc aattgtctga ttcgttacca
      781 attatgacaa cttgacggct acatcattca ctttttcttc acaaccggca cggaactcgc
      841 tcgggctggc cccggtgcat tttttaaata cccgcgagaa atagagttga tcgtcaaaac
      901 caacattgcg accgacggtg gcgataggca tccgggtggt gctcaaaagc agcttcgcct
      961 ggctgatacg ttggtcctcg cgccagctta agacgctaat cccta
//
