LOCUS       sequence-110276-         922 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Yeast CEN/ARS vector (Leu2) that contains dCas9 fused to NLS 
            controlled by TDH3 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 922)
  AUTHORS   Gilbert LA, Larson MH, Morsut L, Liu Z, Brar GA, Torres SE, 
            Stern-Ginossar N, Brandman O, Whitehead EH, Doudna JA, Lim WA, 
            Weissman JS, Qi LS
  TITLE     CRISPR-Mediated Modular RNA-Guided Regulation of Transcription in 
            Eukaryotes.
  JOURNAL   Cell. 2013 Jul 9. pii: S0092-8674(13)00826-X. doi: 
            10.1016/j.cell.2013.06.044.
  PUBMED    23849981
REFERENCE   2  (bases 1 to 922)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2013 
            Jul 9. pii: S0092-8674(13)00826-X. doi: 10.1016/j.cell.2013.06.044."
COMMENT     Sequence Label: pTDH3-dCas9
FEATURES             Location/Qualifiers
     source          1..922
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      120..129
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(158..824)
                     /gene="S. cerevisiae TDH3"
                     /label=GAP promoter
                     /note="promoter for glyceraldehyde-3-phosphate 
                     dehydrogenase; also known as the TDH3 promoter"
     primer_bind     complement(265..288)
                     /label=GPDpro-F
                     /note="S. cerevisiae GPD promoter, forward primer"
     primer_bind     complement(873..892)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(874..892)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(899..916)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(899..915)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 atgccggtcg gtgttcccga gcaccttgaa cttcttagag ggcaccttgt actcgtcagt
       61 gatcacggcc cacccgacgc tattagtccc gatggccagt ccgatagaat acttcttgtc
      121 catggtggcg agatctaatt cgatccatat cagatctttt gtttgtttat gtgtgtttat
      181 tcgaaactaa gttcttggtg ttttaaaact aaaaaaaaga ctaactataa aagtagaatt
      241 taagaagttt aagaaataga tttacagaat tacaatcaat acctaccgtc tttatatact
      301 tattagtcaa gtaggggaat aatttcaggg aactggtttc aacctttttt ttcagctttt
      361 tccaaatcag agagagcaga aggtaataga aggtgtaaga aaatgagata gatacatgcg
      421 tgggtcaatt gccttgtgtc atcatttact ccaggcaggt tgcatcactc cattgaggtt
      481 gtgcccgttt tttgcctgtt tgtgcccctg ttctctgtag ttgcgctaag agaatggacc
      541 tatgaactga tggttggtga agaaaacaat attttggtgc tgggattctt tttttttctg
      601 gatgccagct taaaaagcgg gctccattat atttagtgga tgccaggaat aaactgttca
      661 cccagacacc tacgatgtta tatattctgt gtaacccgcc ccctattttg ggcatgtacg
      721 ggttacagca gaattaaaag gctaattttt tgactaaata aagttaggaa aatcactact
      781 attaattatt tacgtattcn ttgaaatggc agtattgata atgataaact cgaactgact
      841 agtagactga attccgagtg agctccaatt cgccctatag tgagtcgtat tacaattcac
      901 tggccgtcgt tttacaacgt cg
//
