LOCUS       sequence-110392-         844 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Creating Gal4 drivers from MiMIC lines with inserts into coding 
            introns in Phase 1..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Diao F, Ironfield H, Luan H, Diao F, Shropshire WC, Ewer J, Marr E, 
            Potter CJ, Landgraf M, White BH
  TITLE     Plug-and-play genetic access to drosophila cell types using 
            exchangeable exon cassettes.
  JOURNAL   Cell Rep. 2015 Mar 3;10(8):1410-21. doi: 
            10.1016/j.celrep.2015.01.059. Epub 2015 Feb 26.
  PUBMED    25732830
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.01.059"; journalName: "Cell Rep"; date: 
            "2015-03-3- 3"; volume: "10"; issue: "8"; pages: "1410-21"
COMMENT     Sequence Label: pBS-KS-attB2-SA(1)-T2A-Gal4-Hsp70
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    38..107
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     CDS             276..329
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gggcgaattg gagctcgcta gcgatgtagg tcacggtctc gaagccgcgg tgcgggtgcc
       61 agggcgtgcc cttgggctcc ccgggcgcgt actccacctc acccatctgg tccatcatga
      121 tggtcgactc tagaagtcga tccaacatgg cgacttgtcc catccccggc atgtttaaat
      181 atactaatta ttcttgaact aattttaatc aaccgattta tctctcttcc gcaggtggcg
      241 gaggttccgg tggaagcgga ggtagcggcg gatccgaggg ccgcggcagc ctgctgacct
      301 gcggcgatgt ggaggagaac cccgggccca tgaagctgct gagcagcatc gagcaggcct
      361 gcgatatctg ccgcctgaag aagctgaagt gcagcaagga gaagcccaag tgcgccaagt
      421 gcctgaagaa caactgggag tgccgctaca gccccaagac caagcgcagc cccctgaccc
      481 gcgcccacct gaccgaggtg gagagccgcc tggagcgcct ggagcagctg ttcctgctga
      541 tcttcccccg cgaggatctg gatatgatcc tgaagatgga tagcctgcag gatatcaagg
      601 ccctgctgac cggcctgttc gtgcaggata acgtgaacaa ggatgccgtg accgatcgcc
      661 tggccagcgt ggagaccgat atgcccctga ccctgcgcca gcaccgcatc agcgccacca
      721 gcagcagcga ggagagcagc aacaagggcc agcgccagct gaccgtgagc atcgatagcg
      781 ccgcccacca cgataacagc accatccccc tggatttcat gccccgcgat gccctgcacg
      841 gctt
//
