LOCUS       sequence-110450-         753 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Recipient overexpressing vector to construct CFP tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 753)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 753)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC16
FEATURES             Location/Qualifiers
     source          1..753
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        35..433
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     primer_bind     437..453
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     437..453
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(487..515)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 ttaaccctca ctaaagggaa caaaagctgg agctcatagc ttcaaaatgt ttctactcct
       61 tttttactct tccagatttt ctcggactcc gcgcatcgcc gtaccacttc aaaacaccca
      121 agcacagcat actaaatttc ccctctttct tcctctaggg tgtcgttaat tacccgtact
      181 aaaggtttgg aaaagaaaaa agagaccgcc tcgtttcttt ttcttcgtcg aaaaaggcaa
      241 taaaaatttt tatcacgttt ctttttcttg aaaatttttt ttttgatttt tttctctttc
      301 gatgacctcc cattgatatt taagttaata aacggtcttc aatttctcaa gtttcagttt
      361 catttttctt gttctattac aacttttttt acttcttgct cattagaaag aaagcatagc
      421 aatctaatct aagttttcta gaactagtgg atcctaatga gtaaaggaga agaacttttc
      481 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      541 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      601 actactggaa aactacctgt tccatggcca acacttgtca ctactttgac ttggggtgtt
      661 caatgctttg ctagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
      721 cccgaaggtt atgtacagga aagaactata ttt
//
