LOCUS       sequence-110540-         910 bp    DNA     linear   UNA 15-APR-2025
DEFINITION  Vector for expression of St1 sgRNA1.1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Ma H, Naseri A, Reyes-Gutierrez P, Wolfe SA, Zhang S, Pederson T
  TITLE     Multicolor CRISPR labeling of chromosomal loci in human cells.
  JOURNAL   Proc Natl Acad Sci U S A. 2015 Mar 10;112(10):3002-7. doi: 
            10.1073/pnas.1420024112. Epub 2015 Feb 23.
  PUBMED    25713381
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1420024112"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2015-03-10- 10"; volume: "112"; issue: "10"; pages: "3002-7"
COMMENT     Sequence Label: pLH-stsgRNA1.1
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    109..226
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             complement(378..683)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(480..499)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 gagcagccgc gtccctgcgc aaacccaggg ctgccttgga aaaggcgcaa ccccaacccc
       61 ctcgagccgc ggccaaagtg gatctctgct gtccctgtaa taaacccgaa aattttgaat
      121 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
      181 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
      241 tgccatttgt ctcgaggtcg agaattcaaa aaaacaccct gccatagaat gacagggtgt
      301 tgatttcggc atgaagcctt atctttgtag cttctgcatt tctgagagta caaagactgg
      361 tcttcgtcct gcagggttta tattccccag aacatcaggt taatggcgtt tttgatgtca
      421 ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg cacactggcc
      481 atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg gtaaagttca
      541 cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat ccgtcgcccg
      601 ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct ctctctttta
      661 taggtgtaaa ccttaaactg catttcacca gtccctgttc tcgtcagcaa aagagccgtt
      721 catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt tccagcgttc
      781 ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg agatattgac
      841 atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt aatacgctgc
      901 ttcatagcac
//
