LOCUS       sequence-110620-        1121 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1121)
  AUTHORS   Neklesa TK, Tae HS, Schneekloth AR, Stulberg MJ, Corson TW, Sundberg
            TB, Raina K, Holley SA, Crews CM
  TITLE     Small-molecule hydrophobic tagging-induced degradation of HaloTag 
            fusion proteins.
  JOURNAL   Nat Chem Biol. 2011 Jul 3;7(8):538-43. doi: 10.1038/nchembio.597.
  PUBMED    21725302
REFERENCE   2  (bases 1 to 1121)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nchembio"; 
            journalName: "Nat Chem Biol"; date: "2011-07-3- 3"; volume: "7"; 
            issue: "8"; pages: "538-43"
COMMENT     Sequence Label: HA-EGFP-HaloTag2
FEATURES             Location/Qualifiers
     source          1..1121
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             118..144
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             join(148..150,151..861)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(148..150,151..861)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(190..211)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(451..470)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     798..819
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttatgtac
      121 ccttatgacg tacctgacta tgctggagtg agcaagggcg aggagctgtt caccggggtg
      181 gtgcccatcc tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc
      241 gagggcgagg gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc
      301 aagctgcccg tgccctggcc caccctcgtg accaccctga cctacggcgt gcagtgcttc
      361 agccgctacc ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc
      421 tacgtccagg agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag
      481 gtgaagttcg agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag
      541 gaggacggca acatcctggg gcacaagctg gagtacaact acaacagcca caacgtctat
      601 atcatggccg acaagcagaa gaacggcatc aaggtgaact tcaagatccg ccacaacatc
      661 gaggacggca gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc
      721 cccgtgctgc tgcccgacaa ccactacctg agcacccagt ccgccctgag caaagacccc
      781 aacgagaagc gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc
      841 ggcatggacg agctgtacaa ggctcgagat acaaactcat ccgaaatcgg tacaggcttc
      901 cccttcgacc cccattatgt ggaagtcctg ggcgagcgta tgcactacgt cgatgttgga
      961 ccgcgggatg gcacgcctgt gctgttcctg cacggtaacc cgacctcgtc ctacctgtgg
     1021 cgcaacatca tcccgcatgt agcaccgagt catcggtgca ttgctccaga cctgatcggg
     1081 atgggaaaat cggacaaacc agacctcgat tatttcttcg a
//
