LOCUS       sequence-110634-         759 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  when used to produce lentivirus, express physiological levels of 
            insert .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Martz CA, Ottina KA, Singleton KR, Jasper JS, Wardell SE, 
            Peraza-Penton A, Anderson GR, Winter PS, Wang T, Alley HM, Kwong LN,
            Cooper ZA, Tetzlaff M, Chen PL, Rathmell JC, Flaherty KT, Wargo JA, 
            McDonnell DP, Sabatini DM, Wood KC
  TITLE     Systematic identification of signaling pathways with potential to 
            confer anticancer drug resistance.
  JOURNAL   Sci Signal. 2014 Dec 23;7(357):ra121. doi: 
            10.1126/scisignal.aaa1877.
  PUBMED    25538079
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci 
            Signal."; date: "2014-12-23"; volume: "7(357):ra121. doi"; pages: " 
            10.1126/scisignal.aaa1877"
COMMENT     Sequence Label: FLAG-IKKbeta (S177E,S181E)-pcw107
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(43..84)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLGST"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtaatccaga ggttgattgt cgacttaacg cggtcattac tacgtagaac cgagaccgag
       61 gagagggtta gggataggct taccaccggt aaccactttg tacaagaaag ttggcaatca
      121 tgaggcctgc tccaggcagc cccatgtcag ggggtcgagt ccccccaccg cacaaggcct
      181 gctatgggct gccccatgtc agggggtcga gtccccccac atcatgaggc ctgctccagg
      241 cagctgtgct cttcttcttc cgtctgtaac cagctccagt ctagggccgt gaaactctgg
      301 tcttgttccc tcacagtgtc ctgtatggca ttttctagca gggtgcagag gttatgtgct
      361 tcagccacca gttcttcact cttcttggct ggctcaggta agctgttgga ggccgtggag
      421 ggctgagaca tcagctgccc aggctggcta agtcgagagg cattcatgct atccgggctt
      481 ccactgacag gaccacggac cttgctacaa gcaatcttca ggagattcca gagctccttc
      541 tgccgcttct cctgcagccg gacaacagtc ttctcatcct cattcattaa gctcaccacc
      601 tcttccacct tgggcaacag ttccagcgcc ttctgcttgc aaaccacagt tttactgagc
      661 tgcgtataga tcactcgcac tttcttctcg aagctctgaa ttgcctgaag cagcagccgt
      721 accatttcct gactgtcacc ctcagttcgc tggtctcga
//
