LOCUS       sequence-110640-         814 bp    DNA     linear   UNA 14-APR-2025
DEFINITION  Recipient integrative vector to construct mCherry tagged fusions.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Yanez-Carrillo P, Orta-Zavalza E, Gutierrez-Escobedo G, 
            Patron-Soberano A, De Las Penas A, Castano I
  TITLE     Expression vectors for C-terminal fusions with fluorescent proteins 
            and epitope tags in Candida glabrata.
  JOURNAL   Fungal Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020.
  PUBMED    25986172
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Apr 14, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Fungal 
            Genet Biol. 2015 May 15. pii: S1087-1845(15)00087-0. doi: 
            10.1016/j.fgb.2015.04.020."
COMMENT     Sequence Label: pYC56
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..51
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     35..51
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     CDS             55..765
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(200..218)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     468..487
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     protein_bind    complement(772..805)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gggcgaattg gagctccacc gcggtggcgg ccgctctaga actagtggat cctaatggtg
       61 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
      121 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
      181 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
      241 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
      301 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
      361 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
      421 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
      481 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
      541 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
      601 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
      661 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
      721 gagggccgcc actccaccgg cggcatggac gagctgtaca agtagagatc cgaagttcct
      781 atactttcta gagaatagga acttcgtgcg cttt
//
